Molecular Basis of Coactivator Function of RNA helicase A.

RNA 解旋酶 A 共激活子功能的分子基础。

• 批准号: 10480196
• 负责人: NAKAJIMA Toshihiro
• 金额: $ 9.92万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10480196/
• 关键词: transcription; helicase; transcriptional coactivator; integrator; splicing; RNA polymerase II; eukaryotic cell; molecular motor; cellular localization

To understand the molecular basis of eukaryotic gene expression system, I have been focusing on the study of transriptional coactivator complexes, especially a signal coactoivator CREB binding protein (CBP) /RNA helicase A/RNA polymerase II complexes. We found the following ((1)-(9)) and believe that the data will contribute the understanding of eukaryotic gene regulation system.(1) Involvement of RNA helicaseA in complex formation of BRCA1 with RNA polymearse II (Nature Genetics 1998).(2) Implication of glycation of c-Fos by Core 2 N-acetylglycosaminyltransferase on enhanced cytokine actions and induced hypertrophic myocardium in transgenic mice (FASEB. J. 1999).(3) Cooperative role of CBP with transcription factor GATA-1 in erythroid differentiation (PNAS. USA 1998).(4) Transcriptional activatity of an autoimmune protein AIRE and its association with common co-activator CBP (J. B. C. in press 2000).(5) Dual roles of RNA helicase A on CREB-dependent transcription (Journal submitted).(6) Molecular mechanism of nuclear import of RNA helicase A (Journal submitted).(7) Association of TAF130 with poly glutamine protein and its implication in apoptosis of neuronal cells (Nature Genetics revised).(8) Interaction of CBP with beta-catenine (Journal submitted).(9) Hypernuclear acetylation in atherosclerotic lesions and activated vascular smooth muscle cells (Biochem. Biophys. Res. Comm. 1999).

为了了解真核基因表达系统的分子基础，我一直专注于对推进共激活因子复合物的研究，尤其是信号共同激素CREB结合蛋白（CBP） /RNA解旋酶A /RNA聚合酶II复合物II复合物。我们发现了以下（（1） - （9）），并认为数据将有助于理解真核基因调节系统。（1）RNA Helicasea参与BRCA1与RNA Polymearse II的复杂形成（自然遗传学1998）。 （2）CORE 2 N-乙酰基糖胺基因基转移酶对C-FOS的糖基化对增强的细胞因子作用，并诱导转基因小鼠中的肥大性心肌诱导（Faseb。J.，1999）。 （PNAS。USA，1998年）。（4）自身免疫蛋白AIRE的转录活化及其与常见的共激活因子CBP（J. B. C. （6）RNA解旋酶A核进口的分子机制（日记已提交）。（7）TAF130与多谷氨酰胺蛋白的关联及其在神经元细胞凋亡中的含义（修订了自然遗传学）。（8）CBP的相互作用（9）动脉粥样硬化病变和活化的血管平滑肌细胞（Biochem）中的高核乙酰化。生物。 res。通讯。 1999）。

项目成果

Nakajima T.: "Hypernuclear acetylation in Atherosclerotic lesions and activated vascular smooth muscle cell"Biochem. Biophys. Res. Commun.. 266. 417-427 (1999)

Nakajima T.：“动脉粥样硬化病变中的超核乙酰化和激活的血管平滑肌细胞”Biochem。

H. Shin, I. Kitajima, T. Nakajima, Q. Shao, T. Tokioka, I. Takasaki, N. Hanyu, T. Kubo, I. Maruyama.: "Thrombin receptor mediated signals induce expressions of interleukin 6 and granulocyte colony stimulating factor via NF-κB activation in synovial fibrob

H. Shin, I. Kitajima, T. Nakajima, Q. Shao, T. Tokioka, I. Takasaki, N. Hanyu, T. Kubo, I. Maruyama.：“凝血酶受体介导的信号诱导白细胞介素 6 和粒细胞集落的表达通过滑膜纤维中 NF-κB 激活的刺激因子

KP. Sarker, K. Abeyama, JI. Nishi, M. Nakata, T. Tokioka, T. Nakajima, I. Kitajima, I. Maruyama.: "Inhibition of Thrombin-induced Neural Cell Death by Recombinant Thrombomodulin and E5510, a Synthetic Thrombin Receptor Signaling Inhibitor."Thrombosis and

KP。

E.Yoshida: "Identification of N-terminal minimal transcactivation domain of CBP, p300 and Caenorhabditis elegans homologues." Gene. 208. 307-314 (1998)

E.Yoshida：“CBP、p300 和秀丽隐杆线虫同源物 N 末端最小转激活结构域的鉴定。”

M. Miyagishi, T. Nakajima, A. Fukamizu.: "Molecular characterization of mesoderm-restricted basic helix-loop-helix protein, POD-1/capsulin."Medicine. 5. 27-31 (1999)

M. Miyagishi、T. Nakajima、A. Fukamizu.：“中胚层限制性碱性螺旋-环-螺旋蛋白 POD-1/胶囊蛋白的分子特征。”医学。