Development of ultra trace sensing method of vital substances based on new chemical amplification

基于新型化学放大的生命物质超痕量传感方法开发

基本信息

批准号：
10450315
负责人：
IGARASHI Shukuro
金额：
$ 4.03万
依托单位：
IBARAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10450315/
关键词：
Chemical amplification Ultra trace sensing method Porphyrin Peroxidaze Antigen-antibody reaction Labeled-antibody Clinical analysis Simple and visual analytical method ヘモグロビン速度論的計測法

项目摘要

The aim of this study is that creates the high-performance ultra trace sensing method using new chemical amplification as a indicator reaction, which has a characteristic L-shape or S-shape reaction curve. Especially, the target is vital substances which have high analytical needs.The produced fruits during the term of two years are as follows.1. The utilization of L-shape curve based on simultaneous decomposition of catalyst and indicator.Determination of hemoglobin : This established method is able to determine the hemoglobin of concentration range of 12-258 μg/1 and the detection limits (3σ) were 8.13 μg/1. Moreover, the coefficient of variation was 2.0% (determinations), and this result showed that the sensing method using L-shape curve promotes the improvement of analytical precision. The proposed method is that has the best sensitivity compared with already-known many spectrophotometric determinations of hemoglobin.2. The utilization of S-shape curve based on autocatalytic reaction.(1) The simple visual sensing method of peroxidaze was developed using autocatalytic reaction with cobalt-pyridylazo dye complex and peroxomonosulfate. The determination of vital substances is possible by using stop-watch and naked eye. Moreover, this method was applied to the enzyme immunoassay using the label such as peroxidaze.(2) The autocatalytic reaction with sulfite and hydrogen peroxide was used new sensing method. Because of pH signal in indicator reaction are visualized by the use of pH indicator such as bromothumol blue(BTB) and litmus paper, this method has enable determination of tumor necrosis factor in human serum using stop-watch and naked eye.In conclusion, the satisfactory results were obtained in this study according to the original plan. In the near future, this useful method will be applied to wide range of practical sample.

这项研究的目的是使用新的化学放大作为指示反应创建高性能超痕量灵敏度方法，该反应具有特征性的L形或S形反应曲线。尤其是，目标是具有很高分析需求的重要物质。在两年期间生产的果实如下1。基于催化剂和指示剂同时分解L形曲线的利用。血红蛋白的确定性：这种既定方法能够确定浓度范围为12-258μg/1的血红蛋白，检测限（3σ）为8.13μg/1。此外，变异系数为2.0％（确定），该结果表明，使用L形曲线的灵敏度方法促进了分析精度的提高。所提出的方法是与已经知道的血红蛋白的许多分光光度测定相比具有最佳灵敏度。2。基于自催化反应的S形曲线的利用。（1）使用与钴 - 吡啶基唑染料复合物和过氧硫酸盐的自催化反应开发了过氧化物的简单视觉传感方法。通过使用停止观察和肉眼可以确定重要物质。此外，该方法使用过氧化物等标签应用于酶免疫测定。（2）使用了硫酸盐和过氧化氢的自催化反应新的敏感性方法。由于指示反应中的pH信号通过使用pH指示剂（例如溴硫醇蓝色（BTB）和石蕊试纸）可视化，因此该方法实现了使用停止观察和肉眼在人血清中肿瘤坏死因子的测定。结论，根据原始计划，在本研究中获得了令人满意的结果。在不久的将来，这种有用的方法将应用于广泛的实用样本。