Development of ultra trace sensing method of vital substances based on new chemical amplification

基于新型化学放大的生命物质超痕量传感方法开发

基本信息

批准号：
10450315
负责人：
IGARASHI Shukuro
金额：
$ 4.03万
依托单位：
IBARAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10450315/
关键词：
Chemical amplification Ultra trace sensing method Porphyrin Peroxidaze Antigen-antibody reaction Labeled-antibody Clinical analysis Simple and visual analytical method ヘモグロビン速度論的計測法

项目摘要

The aim of this study is that creates the high-performance ultra trace sensing method using new chemical amplification as a indicator reaction, which has a characteristic L-shape or S-shape reaction curve. Especially, the target is vital substances which have high analytical needs.The produced fruits during the term of two years are as follows.1. The utilization of L-shape curve based on simultaneous decomposition of catalyst and indicator.Determination of hemoglobin : This established method is able to determine the hemoglobin of concentration range of 12-258 μg/1 and the detection limits (3σ) were 8.13 μg/1. Moreover, the coefficient of variation was 2.0% (determinations), and this result showed that the sensing method using L-shape curve promotes the improvement of analytical precision. The proposed method is that has the best sensitivity compared with already-known many spectrophotometric determinations of hemoglobin.2. The utilization of S-shape curve based on autocatalytic reaction.(1) The simple visual sensing method of peroxidaze was developed using autocatalytic reaction with cobalt-pyridylazo dye complex and peroxomonosulfate. The determination of vital substances is possible by using stop-watch and naked eye. Moreover, this method was applied to the enzyme immunoassay using the label such as peroxidaze.(2) The autocatalytic reaction with sulfite and hydrogen peroxide was used new sensing method. Because of pH signal in indicator reaction are visualized by the use of pH indicator such as bromothumol blue(BTB) and litmus paper, this method has enable determination of tumor necrosis factor in human serum using stop-watch and naked eye.In conclusion, the satisfactory results were obtained in this study according to the original plan. In the near future, this useful method will be applied to wide range of practical sample.

本研究的目的是创建使用新型化学放大作为反应指示剂的高性能超痕量传感方法，其具有特征性的L型或S型反应曲线，特别是，目标是具有高分析能力的重要物质。两年内的生产成果如下： 1．利用基于催化剂和指示剂同时分解的L型曲线。血红蛋白的测定：本方法可测定浓度范围为12-258 μg/1，检出限（3σ）为8.13 μg/1，并且变异系数为2.0%（测定值），该结果表明使用L形曲线的传感方法促进了分析能力的提高。与已知的多种血红蛋白分光光度测定方法相比，该方法具有最佳的灵敏度。2．利用基于自催化的S型曲线。 (1)利用钴-吡啶偶氮染料络合物和过氧单硫酸盐的自催化反应，建立了简单的过氧化物酶视觉传感方法，并且该方法可以通过秒表和肉眼来测定生命物质。 (2)采用亚硫酸盐和过氧化氢的自催化反应，因为指示剂反应中的pH信号是可视化的。该方法采用溴腐蓝（BTB）等裸pH指示剂和石蕊试纸，实现了秒表和肉眼测定人血清中肿瘤坏死因子的测定。综上所述，本研究按照原法得到了满意的结果。计划在不久的将来，这种有用的方法将应用于广泛的实际样本。