Development of total "down sizing" analytical system following high-speed and high-powered concentration method

遵循高速、高性能浓缩法开发整体“小型化”分析系统

基本信息

批准号：
11555224
负责人：
IGARASHI Shukuro
金额：
$ 8.06万
依托单位：
IBARAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

The aim of this study is that fuses together with the minuteness of sample solution and the analytical apparatus which directs toward "Down Sizing". The homogeneous liquid-liquid extraction was studied in detail as a high-speed and high-powered concentration method, in order to create the total down sizing analytical system.The produced fruits during the term of two years are as follows :1.Development of some chromatographies following homogeneous liquid-liquid extraction.(1)HPLC analytical system : New homogeneous liquid-liquid extraction was developed using pH dependent phase separation phenomenon in water/pyridine/ethyl chloroacetate ternary component solvent system. In this method, the simultaneous determination of metal ions was performed by spectrophotometric detection-HPLC with water-soluble porphyrin.(2)GC/MS analytical system : Above homogeneous liquid-liquid extraction was also applied as pretreatment of GC/MS.As results, twelve kinds of chlorophenol compounds which are doubted as endocrine disrupting chemicals, were simultaneously and rapidly determined.2.Development of some spectrometries following a microdroplet on filter-paper and a homo-geneous liquid-liquid extraction with perfluorosurfactant.(1)Flurescence spectrometry : The pH dependent phase separation using perfluorosurfactant was studied as preconcentration method. Among 22 kinds of amino acid, tryptophan was selectively determined by the fluorometry.(2)X-ray fluorescence spectrometry : Fifteen kinds of element containing high valence metals were simultaneously determined. Moreover, new concept which uses together three kinds of chelating reagents in the same process, was proposed.In conclusion, the satisfactory results were obtained in this project according to the original plan. In the near future, this useful method will be applied to wide range of practical sample.

这项研究的目的是将融合以及样品溶液的小型度和指向“下调”的分析设备。为了创建总降低尺寸的分析系统，详细研究了均匀的液体液体提取方法。（1）HPLC分析系统：使用pH依赖性相分离现象在水/吡啶/吡啶/乙二醇氯乙酸三元组分三元组件解决系统中开发了新的均匀液 - 液体提取系统。在这种方法中，通过分光光度检测-HPLC用水溶性卟啉同时测定金属离子。（2）GC/MS分析系统：上面的均质液体液体提取也被用作GC/MS.AS结果的预测，可以同时且迅速确定的十二种氯苯酚化合物，这些化合物被怀疑是内分泌破坏化学物质的。2。在过滤纸上微弹头后的某些光谱法和均等液态液体提取的某些光谱剂与全氟溶液中的液体液体提取。：使用全荧光外表面活性剂作为预浓缩方法研究了pH依赖相分离。在22种种类的氨基酸中，色氨酸由荧光法选择性确定。（2）X射线荧光光谱法：同时确定了15种包含高价金属的元素。此外，提出了在同一过程中共同使用三种螯合试剂的新概念。总之，根据原始计划在本项目中获得了令人满意的结果。在不久的将来，这种有用的方法将应用于广泛的实用样本。