Breeding of low prolamin rice

低谷醇蛋白水稻的选育

基本信息

批准号：
10356001
负责人：
SATOH Hikaru
金额：
$ 18.01万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10356001/
关键词：
Rice Brewing prosperity prolamin Linkage Analysis Mutation Protein Body cDNA cloning 胚乳貯蔵タンパク質突然変異体 cDNAライブラリー SDS-PAGE 遺伝分析 MNU受精卵処理旺気貯蔵タンパク質

项目摘要

Screening of low prolamin mutantsLow prolamin mutant lines were selected from the progeny of the mutagenesis treatment. The diverse variation for prolamin polypeptides were detected from the native rice varieties, about 2000, collected from some countries. The crossing among low prolamin varieties was done, the progeny is being analyzed.Brewing prosperity of low prolamin mutantThe low prolamin mutant, esp2, showed the low acid degree and low amino acid degree by small scale brewing test, though the mutant showed very high dissolving degree. The results will be helpful on the efficient utilization of the materials.Isolation of the gene for low prolaminBy the results of the linkage analysis of the low prolamin mutant, esp1 gene is positioned within the 1.4 cM region on chromosome 4. The high-density linkage map of esp1 gene will be constructed.The genes of PDI and BiP, related to the accumulation of the prolamin into PBI, were cloned using the cDNA library constructed from the developing seed of rice cultivar 'Kinmaze'. The specific antibodies were prepared based on the cDNA clone.Cytochemical analysis of the low prolamin mutantsProlamin consists of two classes, the cysteine residue poor prolamin (Cys-P prolamin) and the cysteine residue rich prolamin (Cys-R prolamin). The analysis of low prolamin mutants esp3 and Esp4 indicates that Cys-R and Cys-P prolamins are accumulated into PBI at the early and late stages, respectively, during seed development. The analysis by the antibodies to each prolamin classes indicates that Cys-P and Cys-R prolamins accumulate in the center and the periphery of PBI, respectively. It is thought that Cys-P prolamin plays an important role as a core and Cys-R plays the role of filling the inside of PBI.

低谷醇溶蛋白突变体的筛选从诱变处理的后代中选择低谷醇溶蛋白突变体系。从一些国家收集的约 2000 个本土水稻品种中检测到谷醇溶蛋白多肽的多种变异。低谷醇溶蛋白品种间的杂交已完成，后代正在分析中。低谷醇溶蛋白突变体的酿造繁荣低谷醇溶蛋白突变体esp2通过小规模酿造试验显示出低酸度和低氨基酸度，但该突变体表现出很高的溶解度程度。低谷醇溶蛋白基因的分离通过低谷醇溶蛋白突变体连锁分析结果，esp1基因位于4号染色体1.4 cM区域内，呈高密度连锁。构建esp1基因图谱。利用水稻品种发育种子构建的cDNA文库，克隆了与谷醇溶蛋白积累到PBI相关的PDI和BiP基因“金梅兹”。基于cDNA克隆制备特异性抗体。低谷醇溶蛋白突变体的细胞化学分析谷醇溶蛋白由两类组成：缺乏半胱氨酸残基的谷醇溶蛋白(Cys-P谷醇溶蛋白)和富含半胱氨酸残基的谷醇溶蛋白(Cys-R谷醇溶蛋白)。对低谷醇溶蛋白突变体esp3和Esp4的分析表明，Cys-R和Cys-P谷醇溶蛋白分别在种子发育过程的早期和晚期阶段积累到PBI中。对每种谷醇溶蛋白类别的抗体进行的分析表明，Cys-P 和 Cys-R 谷醇溶蛋白分别积聚在 PBI 的中心和外围。 Cys-P谷醇溶蛋白被认为作为核心发挥着重要作用，Cys-R则发挥着填充PBI内部的作用。