Study on glutelin genes of storage proteins in rice.

水稻贮藏蛋白谷蛋白基因的研究.

基本信息

批准号：
04454041
负责人：
SATOH Hikaru
金额：
$ 4.1万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454041/
关键词：
Rice Improvement of grain properties Genetic resources Storage protein Glutelin Gene analysis SDS-PAGE IEF グリテリン SOS-PAGE 突然変異体

项目摘要

Two major subunits of rice glutelin, the acidic (alpha) and basic (beta) subunits consist of various polypeptides in their apparent molecular mass and pI.We found a wide variation among the rice atrains in the apparent molecular mass and pI by SDSPAGE and IEF,especially between Japonica and Indica types. The glutelin of "Kinmaze", Japonica type, and "IR36", Indica type, were analyzed exactly. In SDSPAGE analysis, the glutelin was separated into four alpha subnit bands, alpha-1, alpha-2, alpha-3 and alpha-4, and four beta subunit bands, beta-1, beta-2, beta-3 and beta-4.The alpha-3 of "Kinmaze" moved faster than that of "IR36". In IEF analysis, alpha and beta subunits had at least 12 and 9 bands, respectively. Several polymorphic hands were detected in both subunits. The relation between the apparent molecular mass and pI of the polypeptides were analyzed by 2-dimensional electrophoresis. Each SDS-PAGE band consists of some polypeptides showing pI heterogeneity, while some polypeptides … More of same pI showed different molecular weight. The polymorphic bands in IEF were detected as each single spot, and belong to alpha-2 and beta-2. The genetic analysis of the polymorphic bands were done by IEF using "Kinmaze", "IR24" and RI (recombinant inbred) lines. "IR24" shows almost the same pattern with "IR36", but has another band in alpha subunit. RI lines were made from a cross seed between "Asominori", showing the same pattern with "Kinmaze", and "IR24". The bands of reciprocal F1 hybrid seeds between "Kinmaze"and "IR24"showed the gene dosage effect. In F2 analysis, segregation of the bands, phenotypes in staining intensity, fitted to a ratio of 1 : 1 : 1 : 1 (or 3 : 1), indicating these bands were controlled by incomplete dominant genes. Recombinants were found among the bands, indicating each band was controlled by each incomplete gene. The linkage relationship of the genes controlling each polymorphic bands were found in both genes controlling alpha-2 polypeptides and alpha-3 and beta-2 polypeptides. Then chromosomal location of these genes was studied by means of RI lines. The genes controlling alpha-2 polypeptides were shown to be linked with RFLP markers located on the chromosome 1, while The genes controlling alpha-3 and beta-2 polypeptides were shown to be linked with RFLP markers located on the chromosome 2. Less

水稻谷蛋白的两个主要亚基，酸性亚基（α）和碱性亚基（β）由表观分子质量和等电点不同的多肽组成。通过SDSPAGE和IEF，我们发现水稻谷蛋白的表观分子质量和等电点存在很大差异。 ,特别是在粳稻和籼稻类型之间,对粳稻类型“Kinmaze”和籼稻类型“IR36”的谷蛋白进行了准确的SDSPAGE分析。分析中，谷蛋白被分成四个α亚基带，α-1、α-2、α-3和α-4，以及四个β亚基带，β-1、β-2、β-3和β-4。 “Kinmaze”的α-3比“IR36”的移动速度更快，在IEF分析中，α和β亚基分别具有至少12和9条带，在两个亚基中检测到了明显的关系。通过二维电泳分析多肽的分子量和 pI。每个 SDS-PAGE 条带由一些显示 pI 异质性的多肽组成，而某些相同 pI 的多肽则在 IEF 中检测到不同的多态性条带。单点，属于α-2和β-2。多态性条带的遗传分析是使用“Kinmaze”、“IR24”和RI（重组体）进行的。 “IR24”显示与“IR36”几乎相同的模式，但在 α 亚基中具有另一个条带，该 RI 系由“Asominori”之间的杂交种子制成，显示与“Kinmaze”和“IR24”相同的模式。 “Kinmaze”和“IR24”之间的倒数 F1 杂交种子的条带显示了基因剂量效应。在 F2 分析中，条带的分离、染色强度的表型，拟合为比例为1:1:1:1（或3:1），表明这些条带是由不完整的显性基因控制的，条带之间发现了重组体，表明每个条带由每个不完整的基因控制。在控制α-2多肽和α-3和β-2多肽的两个基因中都发现了控制每个多态性条带，然后通过RI系研究了这些基因的染色体定位。显示与位于 1 号染色体上的 RFLP 标记连锁，而控制 alpha-3 和 beta-2 多肽的基因显示与位于 2 号染色体上的 RFLP 标记连锁。