Study on glutelin genes of storage proteins in rice.

水稻贮藏蛋白谷蛋白基因的研究.

基本信息

批准号：
04454041
负责人：
SATOH Hikaru
金额：
$ 4.1万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454041/
关键词：
Rice Improvement of grain properties Genetic resources Storage protein Glutelin Gene analysis SDS-PAGE IEF グリテリン SOS-PAGE 突然変異体

项目摘要

Two major subunits of rice glutelin, the acidic (alpha) and basic (beta) subunits consist of various polypeptides in their apparent molecular mass and pI.We found a wide variation among the rice atrains in the apparent molecular mass and pI by SDSPAGE and IEF,especially between Japonica and Indica types. The glutelin of "Kinmaze", Japonica type, and "IR36", Indica type, were analyzed exactly. In SDSPAGE analysis, the glutelin was separated into four alpha subnit bands, alpha-1, alpha-2, alpha-3 and alpha-4, and four beta subunit bands, beta-1, beta-2, beta-3 and beta-4.The alpha-3 of "Kinmaze" moved faster than that of "IR36". In IEF analysis, alpha and beta subunits had at least 12 and 9 bands, respectively. Several polymorphic hands were detected in both subunits. The relation between the apparent molecular mass and pI of the polypeptides were analyzed by 2-dimensional electrophoresis. Each SDS-PAGE band consists of some polypeptides showing pI heterogeneity, while some polypeptides … More of same pI showed different molecular weight. The polymorphic bands in IEF were detected as each single spot, and belong to alpha-2 and beta-2. The genetic analysis of the polymorphic bands were done by IEF using "Kinmaze", "IR24" and RI (recombinant inbred) lines. "IR24" shows almost the same pattern with "IR36", but has another band in alpha subunit. RI lines were made from a cross seed between "Asominori", showing the same pattern with "Kinmaze", and "IR24". The bands of reciprocal F1 hybrid seeds between "Kinmaze"and "IR24"showed the gene dosage effect. In F2 analysis, segregation of the bands, phenotypes in staining intensity, fitted to a ratio of 1 : 1 : 1 : 1 (or 3 : 1), indicating these bands were controlled by incomplete dominant genes. Recombinants were found among the bands, indicating each band was controlled by each incomplete gene. The linkage relationship of the genes controlling each polymorphic bands were found in both genes controlling alpha-2 polypeptides and alpha-3 and beta-2 polypeptides. Then chromosomal location of these genes was studied by means of RI lines. The genes controlling alpha-2 polypeptides were shown to be linked with RFLP markers located on the chromosome 1, while The genes controlling alpha-3 and beta-2 polypeptides were shown to be linked with RFLP markers located on the chromosome 2. Less

水稻谷蛋白的两个主要亚基，酸性（α）和碱性（β）亚基在其明显的分子质量和PI中由各种多肽组成。我们发现，在SDSPAGE和IEF中，米饭ATRAIN的大米ATRAIN在SDSPAGE和IEF中，尤其是Japonica和Indica类型之间的变化。精确地分析了“ Kinmaze”，Japonica类型和“ IR36”的麸质蛋白。在SDSPAGE分析中，将麸质蛋白分成四个Alpha子谱带，Alpha-1，Alpha-2，Alpha-3和Alpha-4，以及四个Beta亚基频段，Beta-1，Beta-1，Beta-2，Beta-3和Beta-3和Beta-4。在IEF分析中，Alpha和Beta亚基分别具有至少12个和9个频段。在两个亚基中都检测到了几种多态手。通过二维电泳分析了明显的分子质量与多肽的PI之间的关系。每个SDS-PAGE谱带由一些显示PI异质性的多肽组成，而某些多肽……更多相同的PI显示出不同的分子量。检测到IEF中的多态性带作为每个位置，属于alpha-2和beta-2。使用“ kinmaze”，“ ir24”和ri（重组近交）线进行了IEF进行多态性带的遗传分析。 “ IR24”与“ IR36”显示了几乎相同的模式，但在Alpha亚基中有另一个乐队。 RI线是由“ Asominori”之间的交叉种子制成的，与“ Kinmaze”和“ IR24”显示了相同的模式。 “ kinmaze”和“ ir24”之间的相互F1杂交种子的带显示了基因剂量效应。在F2分析中，频带的分离，染色强度中的表型，拟合为1：1：1：1：1：1（或3：1）的比例，表明这些条带受到不完全的主导基因的控制。在频段中发现重组剂，表明每个频带都由每个不完全基因控制。在控制α-2多肽和α-3和β-2多肽的基因中都发现了控制每个多态性带的基因的连锁关系。然后通过RI系研究了这些基因的染色体位置。显示控制α-2多肽的基因与位于1号染色体上的RFLP标记相关，而控制α-3和Beta-2多肽的基因与位于2号染色体上的RFLP标记相关。