Study on the sequence and determination of the allelic variants at the D1S80 locus

D1S80位点等位基因变异的序列研究和测定

• 批准号: 10307011
• 负责人: FUKUSHIMA Hirofumi
• 金额: $ 11.97万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10307011/
• 关键词: PCR; subtyping; paternity testing; polymorphism; DNA; D1S80; sequence; variants; DIS80; 蛍光色素; 多型; DNA; 塩基配列

Through analyzing the alleles in Japanese and Chinese populations, we established a new nomenclature for the PCR-RFLP band patterns, closely relating them to their corresponding sequences so that the new types could be designated easily and accurately. The short fragments of 16 bp, 32 bp, 48 bp, 64 bp, and 80 bp contained 1,2,3,4, and 5 repeat units, respectively, and were thus named bands "1", "2", "3", "4", and "5". Excluding a flanking region, the eight kinds of long fragments of 221 bp, 237 bp, 253 bp, 269 bp, 285bp, 317 bp, 349bp, and 365 bp in length each contained 6,7,8,9,10,12,14, and 15 repeat units.To testify whether alleles of the same length and of the same band pattern possessed the same sequences, a number of alleles were sequenced in addition to the above-sequenced samples. Of the sequenced samples which had the same lengths and the same band patterns, most shared the same sequences. However, some revealed different sequences. Because of the differences in the repeat arrays between the variants and the ladder markers, structural differences or one-sided destribution of the electrical charge led to more apparent migration in polyacrylamide gel electrophoresis.

通过分析日本和中国人群中的等位基因，我们建立了一个针对PCR-RFLP频段模式的新命名法，将它们与它们的相应序列紧密相关，以便可以轻松，准确地指定新类型。 16 bp，32 bp，48 bp，64 bp和80 bp的短片段分别包含1,2,3,4和5个重复单元，因此被命名为“ 1”，“ 2”，“ 3”。 “，“ 4”和“ 5”。不包括侧翼区域，221 bp，237 bp，253 bp，269 bp，269 bp，285bp，317 bp，349bp和365 bp的八种长片段，长度为6,7,8,9,10,10,12， 14和15个重复单元。证明相同长度和相同带模式的等位基因是否具有相同的序列，除了上述样品外，还测序了许多等位基因。在具有相同长度和相同带模式的测序样品中，大多数共享了相同的序列。但是，有些揭示了不同的序列。由于变体和梯子标记之间的重复阵列差异，因此电荷的结构差异或单侧破坏导致聚丙烯酰胺凝胶电泳中更明显的迁移。

项目成果

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Takayanagi, K.、Hanawa, K.、Liu, C.、Asamura, H.、Ota, M.、Fukushima, H.：“线粒体 DNA 分析：使用两名失踪人员脱落的头发进行身份识别。”Res.Pract

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