Studies of Rh antigen-related gene (RNA and DNA) and its Medico-Legal application

Rh抗原相关基因（RNA和DNA）的研究及其医疗法律应用

• 批准号: 61480176
• 负责人: FUKUSHIMA Hirofumi
• 金额: $ 4.03万
• 依托单位: Dept. of Legal Medicine, Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480176/
• 关键词: DNA analysis; mRNA; Rh antigen; LW antigen; RIA; ELISA; クローニング; DNA解析; Lh抗原

Enzyme-linked immumosorbent assay (ELISA) was used for ABO,Rh,fy,Jk,K Blood grouping. Positive and negatine reactions of anti-A, anti-B, anti-D, anti-C, anti-Fya, anti-Jka, and anti-K wells were ensily distinguished by this method. As pant of a study of frozm red cells, we tested the stability of the ABO and Rh antigens in frozem ulls. There were no significant changes of 125I-proteinA binding counts. There appear to be no barriers to the use of frozem uellss for fomily study or Rh blood type antigens. In order to isolate mRNA which is related to Rh 25(LW) antigen, we are now trying to purify total RNA from Japanese monky spleen. To obtain mRNA from exuss of other RNA, we are going to separate the RNA by chronatography on an oligo-(dT)-cellulose column.

酶联分析（ELISA）用于ABO，RH，FY，JK，K血分组。抗A，抗B，抗D，抗C，抗fya，anti-jka和抗K井的阳性和负反应被这种方法区分了。作为对冻细胞研究的研究，我们测试了Frozem Ull中ABO和RH抗原的稳定性。 125-proteina结合计数没有显着变化。似乎没有使用冷冻UELLS进行Fomily研究或RH血型抗原的障碍。为了分离与RH 25（LW）抗原相关的mRNA，我们现在正在尝试净化日本Monky Spleen的总RNA。为了从其他RNA的exuss获得mRNA，我们将通过寡纤维（DT） - 纤维素柱上的表格分离RNA。

项目成果

Hirofumi Fukushima: Jpn J Legal Med. 40. 366-369 (1986)

福岛博文：Jpn J Legal Med。

Hirofumi Fukushima: Act. Crim. Japon. 53. 244-247 (1987)

福岛博文：行动。

Hirofumi,Fukushima: "Localization of Rh 1(D),2(C),3(E),4(c),5(E) and 25(LW) antigens of humar Rh blood groups in fetal erythrocyte membranes" Comp. Buichem. Physial.86B. 303-305 (1987)

Hirofumi，Fukushima：“胎儿红细胞膜中人类 Rh 血型的 Rh 1(D)、2(C)、3(E)、4(c)、5(E) 和 25(LW) 抗原的定位”比较。

Hirofumi,Fukushima: "A radioimmunoassay using 125I-proteinA for the cletermination of red cell Rh blood group antigens, Rn2(C),Rh3(E),Rh4(C) and Bh5(e)." Act. Crim. Japan. 53. 43-46 (1987)

Hirofumi，Fukushima：“使用 125I-蛋白 A 来测定红细胞 Rh 血型抗原 Rn2(C)、Rh3(E)、Rh4(C) 和 Bh5(e) 的放射免疫测定法。”

Hirofumi Fukushima: J. Forens. Sa. 32. 531-534 (1987)

福岛博文：J. Forens。