Glial role in neuronal development and function

胶质细胞在神经元发育和功能中的作用

基本信息

批准号：
10214201
负责人：
WATANABE Masahiko
金额：
$ 18.37万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

项目摘要

The Bergmann glia (BG) is unipolar cerebellar astrocytes associating with migrating granule cells (GCs) and mature Purkinje cells (PCs). By taking cytochemical advantage of glutamate transporter GLAST localized on the plasmalemma of raidal glia/astrocyte lineage, we analyzed the morphodifferentiation of BG. Cells expressing GLAST mRNA were present only in the ventricular zone at embryonic day 13 (El3). At E14, they first appeared in the mantle zone, where they had oval to elongated cell bodies and radial fibers reaching the pial surface, suggesting the migration of cerebellar radial glia. From E18 to P7, GLAST-expressing cells in the cortex established characteristics of BG (i.e., monolayer alignment around PC bodies and expression of glial fibrillary acidic protein in Bergmann fibers) and further up-regulated GLAST expression, whereas those in other cerebellar regions were changed into multipolar astrocytes and down-regulated the expression. From P7 to P21, Bergmann fibers were change … More d from linear to reticular form in a deep-to-superficial gradient; the superficial linear portion was intimately contacted with the growing tips of PC dendrites, while the deeper reticular portion reflected active formation of membranous protrusions invading the neuropil elaborately to surround PC synapses. Following the accomplishment as unipolar cortical astrocytes, therefore, Bergmann fibers of BG undergo dynamic transformation in the active synaptogenic phase, leading to structural maturation of PC synapses. Intimate contacts with dendritic tips further suggest that Bergmann fibers could be a structural substrate for arborizing PC dendrites as well as for migrating GCs.To further pursue the molecular basis, neuron-glial relationship on serine metabolism was examined by producing antibody to serine synthetic enzyme 3-phosphoglycerate dehydrogenase (3PGDH). 3PGDH was highly and selectively expressed throughout development in astrocytes and olfactory ensheathing glia. In the cerebellum, the growing tips of 3PGDH-negative PC dendrites were contacted to 3PGDH-positive Bergmann fibers in developing cerebellum. These results suggest that serine biosynthesis by BG and its supply to PCs are one of the plausible reason for the intimate structural relationships between dendrites and glial fibers during development. Less

Bergmannn Glia（BG）是迁移的颗粒细胞（GC）和成熟的Purkinje细胞（PC）的单极结合（PCS）。在E14的胚胎第13天，它们首先出现在地幔区域。在伯格曼纤维中的神经纤维纤维酸性蛋白质的表达和进一步的上限胶状表达，而其他小脑区域的表达则呼唤到多层型小脑区域，并从p7到p21 e d。表面梯度;浅表线性部分与PC树突的增长接触，而较深的维特式部分活性形成围绕PC突触。 PC突触的成熟。和嗅觉开发胶质，3pgdh阴性的PC树突的增长尖端o 3pgdh阳性的伯格曼纤维在开发小脑时，丝氨酸生物合成及其对PC的供应。在发育过程中，树突和神经胶纤维之间的一些ntimate结构关系