Control of movements by the cerebellum

小脑对运动的控制

• 批准号: 10842088
• 负责人: REZA SHADMEHR
• 金额: $ 6.6万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-15 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10842088
• 关键词: Adolescence; Animals; Award; Behavioral; Biomedical Engineering; Callithrix; Cell Nucleus; Cells; Cerebellar Diseases; Cerebellum; Childhood; Disease model; Eligibility Determination; Health; Link; Modeling; Movement; Neurons; Purkinje Cells; Research; Research Project Grants; Technology; Testing; awake; economic disparity; embryonic stem cell; graduate student; medical schools; nonhuman primate; programs; undergraduate student

This is a request for a Administra�ve Supplement to Promote Diversity in Health-Related Research for the currently ac�ve award 1R37NS128416 to support Alden Naeem, a first-year graduate student in the Biomedical Engineering Program at the Johns Hopkins School of Medicine. Her eligibility for the diversity supplement is based on economic disadvantages that she endured throughout her childhood, adolescence, and undergraduate years. The research project that we propose for Alden Naeem is to produce a model of cerebellar disease in a non-human primate, and then use the model to quan�fy ac�vi�es of cells in the model-diseased cerebellum in awake behaving animals. If successful, this will result in a first-ever model of episodic cerebellar disease in a non-human primate. The approach that we will employ is to use chemogene�c technology to transiently, and reversibly, manipulate the ac�vity of Purkinje cells in the cerebellum of marmosets. A sub aim is to compare the ac�vi�es of cells in the cerebellum before and a�er Purkinje cell silencing. Importantly, we will be able to do this comparison in the same neuron because the effects of the chemogene�c silencing are short lived. By recording the ac�vi�es of neurons in the cerebellum when the P-cells are silenced, we will be able to test for a causal link between modula�on of spike rates in the P-cells and their downstream nucleus neurons, and behavioral changes such as endpoint accuracy of movements.

这是促进与健康相关研究多样性的行政补充的要求 目前授予1R37NS128416授予Alden Naeem，这是一年级的研究生 约翰·霍普金斯医学院的生物医学工程计划。她有资格获得多样性 补充是基于她在整个童年时期遭受的经济灾难， 青少年和本科生。我们为Alden Naeem提出的研究项目是 在非人类的灵长类动物中产生小脑疾病模型，然后使用该模型进行Quanfy 模型鉴定的小脑在清醒的动物中的细胞AC。如果成功，这将 导致在非人类灵长类动物中有史以来第一种偶发性小脑疾病模型。 我们将采用的方法是使用化学生成技术瞬时，可逆地 操纵果marmosets小脑中浦肯野细胞的尖锐性。一个次目标是比较 小脑中细胞的AC和AR Purkinje细胞沉默。重要的是，我们将能够 在同一神经元中进行比较，因为化学遗传沉默的影响很短 生活。通过在p细胞沉默时记录小脑中神经元的ACS，我们将是 能够测试P细胞中的尖峰速率模量及其下游之间的因果关系 核神经元和行为变化，例如运动的终点精度。