Evaluation of oocytes activation by oocyte-ativating factors derived from sperm extract.

精子提取物中的卵母细胞激活因子对卵母细胞激活的评估。

基本信息

批准号：
08660352
负责人：
HOSOI Yoshihiko
金额：
$ 1.41万
依托单位：
Kinki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660352/
关键词：
microfertilization pronucleous formation oocyte activating factor 顕微受精

项目摘要

In mammalian oocytes, fertilization-associated calcium [Ca2+]i oscillations are responsible for the activation of development. It has been proposed that the sperm may interact with receptors in the oocyte's plasma membrane and engage intracellular signaling pathways that result in Ca2+ release. A different line of investigation suggests that upon sperm-oocyte fusion, a sperm cytosolic factor is released into the oocyte which interacts with unknown cytosolic targets, and generates [Ca2+]i oscillations. In this study, we demonstrated that injections of xeno-sperm crude extracts (SF) into other mammalian oocytes couses activation and in vitro parthenogenetic development. A hypothesis we supported in the mechanism of signal transduction at fertilization proposes that a factor from the sperm (SF) is released into the egg and, by interacting with unknown cytosolic targets, generates activation of oocytes. Injection of various sperm into hamster, mouse, rabbit, pig and bovine oocytes (this me … More ans that gametes' membrane fusion was by-passed) activated oocytes similar to those observed during fertilization, in our study. To further test the physiological relevance of SF,SF is obtained from porcine sperm were injected into hamster oocytes. It was caused pronuclear formation but formation is delayd compared to that of normal hamster fertilization. Probably it was due to fertilized hamster oocytes oscillate with higher frequency than porcine oocytes. Thus, if SF contains a physiological initiator of [Ca2+]i oscillations, injection of SF from one species into oocytes of a different species should be able to induce Ca2+ responses in these oocytes and it should respect the pattern characteristic of each species. Long lasting oscillations are characteristic of mammalian fertilization and they are critical for the activation of embryonic development. It may triggered by sperm cytosolic factors. For example, stimulation of this pathway may be used for parthenogenetic activation in embryo cloning and its inhibition may offer insights in contraception. Less

在哺乳动物卵母细胞中，与受精相关的钙 [Ca2+]i 振荡负责发育的激活，有人提出，精子可能与卵母细胞质膜中的受体相互作用，并参与导致 Ca2+ 释放的细胞内信号传导途径。研究表明，精子-卵母细胞融合后，精子胞质因子被释放到卵母细胞中，与未知的胞质靶标相互作用，并产生 [Ca2+]i在这项研究中，我们证明将异种精子粗提物（SF）注射到其他哺乳动物卵母细胞中会引起激活和体外孤雌发育，我们在受精信号转导机制中支持的假设表明，来自精子的因素。 (SF) 被释放到卵子中，通过与未知的胞质靶标相互作用，激活卵母细胞。将各种精子注射到仓鼠、小鼠、兔子、猪和牛的卵母细胞中。（这就是配子的膜融合被绕过）激活的卵母细胞与受精过程中观察到的类似，在我们的研究中，为了进一步测试 SF 的生理相关性，将从猪精子中获得的 SF 注射到仓鼠卵母细胞中。它引起原核形成，但与正常仓鼠受精相比，其形成延迟，这可能是由于受精仓鼠卵母细胞的振荡频率高于猪卵母细胞。因此，如果 SF 含有 [Ca2+]i 振荡的生理引发剂，则将一个物种的 SF 注射到不同物种的卵母细胞中应该能够在这些卵母细胞中诱导 Ca2+ 反应，并且应该尊重每个物种的模式特征。振荡是哺乳动物受精的特征，对于胚胎发育的激活至关重要，它可能由精子胞质因子触发，例如，刺激该途径可用于胚胎克隆中的孤雌生殖激活。其抑制作用可能为避孕提供启示。