Evaluation of oocytes activation by oocyte-ativating factors derived from sperm extract.

精子提取物中的卵母细胞激活因子对卵母细胞激活的评估。

• 批准号: 08660352
• 负责人: HOSOI Yoshihiko
• 金额: $ 1.41万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660352/
• 关键词: microfertilization; pronucleous formation; oocyte activating factor; 顕微受精

In mammalian oocytes, fertilization-associated calcium [Ca2+]i oscillations are responsible for the activation of development. It has been proposed that the sperm may interact with receptors in the oocyte's plasma membrane and engage intracellular signaling pathways that result in Ca2+ release. A different line of investigation suggests that upon sperm-oocyte fusion, a sperm cytosolic factor is released into the oocyte which interacts with unknown cytosolic targets, and generates [Ca2+]i oscillations. In this study, we demonstrated that injections of xeno-sperm crude extracts (SF) into other mammalian oocytes couses activation and in vitro parthenogenetic development. A hypothesis we supported in the mechanism of signal transduction at fertilization proposes that a factor from the sperm (SF) is released into the egg and, by interacting with unknown cytosolic targets, generates activation of oocytes. Injection of various sperm into hamster, mouse, rabbit, pig and bovine oocytes (this me … More ans that gametes' membrane fusion was by-passed) activated oocytes similar to those observed during fertilization, in our study. To further test the physiological relevance of SF,SF is obtained from porcine sperm were injected into hamster oocytes. It was caused pronuclear formation but formation is delayd compared to that of normal hamster fertilization. Probably it was due to fertilized hamster oocytes oscillate with higher frequency than porcine oocytes. Thus, if SF contains a physiological initiator of [Ca2+]i oscillations, injection of SF from one species into oocytes of a different species should be able to induce Ca2+ responses in these oocytes and it should respect the pattern characteristic of each species. Long lasting oscillations are characteristic of mammalian fertilization and they are critical for the activation of embryonic development. It may triggered by sperm cytosolic factors. For example, stimulation of this pathway may be used for parthenogenetic activation in embryo cloning and its inhibition may offer insights in contraception. Less

在哺乳动物的卵母细胞中，受精相关的钙[Ca2+] I振荡负责发育的激活。已经提出，精子可以与卵母细胞质膜中的接收器相互作用，并参与导致Ca2+释放的细胞内信号通路。另一项研究表明，在精子融合时，精子胞质因子被释放到卵母细胞中，该因子与未知的胞质靶标相互作用，并产生[Ca2+] I振荡。在这项研究中，我们证明了Xeno-Sperm原油提取物（SF）注射其他哺乳动物卵母细胞的激活和体外的孤雌激活。我们在受精建议下信号转导的机理中支持的一个假设，即精子（SF）的因子被释放到卵中，并通过与未知的胞质靶标相互作用会产生卵母细胞的激活。在我们的研究中，将各种精子注入仓鼠，小鼠，兔子，猪和牛卵母细胞（这是我的……更多的ANS膜融合）激活的卵母细胞在我们的研究中类似于受精期间观察到的卵母细胞。为了进一步测试SF的物理相关性，将SF从猪精子中获得的SF注入仓鼠卵母细胞。它是引起核形成的，但与正常仓鼠受精相比，形成延迟。这可能是由于受精仓鼠卵母细胞的振荡量高于猪卵母细胞。如果SF包含[Ca2+] I振荡的物理启动因子，则将SF从一个物种注射到不同物种的卵母细胞中应该能够在这些卵母细胞中诱导Ca2+反应，并且应尊重每个物种的模式特征。持久的振荡是哺乳动物施肥的特征，它们对于胚胎发育的激活至关重要。它可能是由精子胞质因子触发的。例如，对该途径的刺激可用于胚胎克隆中的孤态激活，其抑制作用可能会提供偶然性的见解。较少的

项目成果

Y.Hosoi: "Reproductive Biology Update" H.Miyamoto & N.Manabe, 498 (1998)

Y.Hosoi：“生殖生物学更新” H.Miyamoto

細井美彦: "ブタ精子由来の卵子活性化因子の検討" 日本胚移植学雑誌. (印刷中). (1998)

Yoshihiko Hosoi：“来自猪精子的卵子激活因子的研究”，《日本胚胎移植杂志》（出版中）。

Y.Hosoi & A.Iritani: "Fertilization in vitro by microinjection of sperm head in the rabbit and cattle." Mem.School.BasT.Kinki University. 1. 59-65 (1997)

细井Y

Y.Hosoi: "Microinjection of domestic and zoo animalspermatozoa into oocytes and their development." Zygote. (in press). (1998)

Y.Hosoi：“将家畜和动物园动物的精子显微注射到卵母细胞中及其发育。”

Y.Hosoi: "Evaluation of oocytes activating activity by porcine sperm extract." Jpn.J.Embryo Trans.(in print). (1998)

Y.Hosoi：“猪精子提取物对卵母细胞激活活性的评估。”