Effects of oocyte activation factors derived from spermatozoa on the embryo development.

精子来源的卵母细胞激活因子对胚胎发育的影响。

• 批准号: 12660263
• 负责人: HOSOI Yoshihiko
• 金额: $ 1.09万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660263/
• 关键词: Intracytoplasmic Sperm Injection; Activation factor; Rabbit Oocyte; Calcium oscillation; Cynomologus Monkey; Additional stimulation; Electric Stimulation; 卵子活性化条件; 胚発生; 精子由来活性化因子; 活性化能力; ハムスター精子抽出液; 発生能力の改善; Intracytoplasmic Sperm Injection; Activation factor; Rabbit Oocyte; Calcium oscillation; Cynomologus Monkey; Additional stimulation; Electric Stimulation; 卵子活性化条件; 胚発生; 精子由来活性化因子; 活性化能力; ハムスター精子抽出液; 発生能力の改善

During fertilization, sperm entry triggers a series of intracellular calcium oscillations critical to oocyte activation. This activation is prerequisite to the resumption and completion of meiosis, subsequent pronuclear formation and DNA synthesis.Intracytoplasmic sperm injection (ICSI) has been applied to human infertility treatment. This technique could be also useful for determining the roles of various sperm components in fertilization. Although ICSI is usually a highly successful treatment for severe male factor infertility, some cases repeatedly experience low ICSI fertilization rates (<50%). Possible causes include failure of oocyte activation resulting from sperm and/or oocyte defects. Previous studies have shown that oocytes injected with round spermatids (Hosoi et al., 2000) can be activated by the calcium ionophore A23187.Various artificial treatments mimic sperm-triggered events and induce parthenogenetic development in MII oocytes. It suggests that additional stimulation helps development of fertilized eggs under insufficient condition. In considering the application of intracytoplasmic sperm injection (ICSI) for endangered species and infertility patients, in many cases only not adequate gametes are expected to be used for reproduction. In conclusion, our study showed that oocytes activation factors was very important for artificial fertilization.

在受精过程中，精子进入会触发一系列对卵母细胞激活至关重要的细胞内钙振荡。这种激活是减数分裂，随后的前核形成和DNA合成的先决条件。内部质子精子注射（ICSI）已应用于人类不育症治疗。该技术对于确定各种精子成分在受精中的作用也可能很有用。尽管ICSI通常是严重男性不育症的非常成功的治疗方法，但某些病例反复经历较低的ICSI受精率（<50％）。可能的原因包括精子和/或卵母细胞缺陷引起的卵母细胞激活失败。先前的研究表明，可以通过钙离子载体A23187激活注射圆形精子的卵母细胞（Hosoi et al。，2000）。多种人工处理模仿了精子触发的事件，并诱导MII OOCYTES中的parthenenogenogenatic发育。这表明额外的刺激有助于在状况不足的情况下发展受精卵。在考虑将胞质内精子注射（ICSI）应用于濒危物种和不育患者时，在许多情况下，只有不够的配子被预计将用于繁殖。总之，我们的研究表明，卵母细胞激活因子对于人工施肥非常重要。