Study on the flower color change by measuring vacuolar pH

测定液泡pH值研究花色变化

• 批准号: 08660141
• 负责人: YOSHIDA Kumi
• 金额: $ 1.41万
• 依托单位: Sugiyama Jogakuen University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660141/
• 关键词: Ipomoea tricolor; vacuolar pH; pH sensitive microelectrode; protoplast; V-ATPase; floser color change; vacuolar membrane; PPase; 細胞内微少pH電極; 西洋アサガオ; プロトプスト; プロトンポンプ

The petal of blue morning glory, Ipomoea tricolor cv. heavenly blue, changes its color from purplish red to light blue during blooming. The pigment, heavenly blue anthocyanin, was distributed in the vacuoles of upper and lower epidermis and not in parenchyma. We measured pHv of living petal cell directly using proton selective-microelectrode. The pHv of blue open flower was 7.7 while that of purplish red buds was 6.7. This study provides the clear evidence that the color change is caused by the increase of pHv during blooming.To clarify the pH increasing mechanism the colored protoplasts were prepared from the petals of purplish-red buds, blue open flower and purple faded flower, followed by the vacuolar membrane were provided. By immunoblot technique H^+-ATPase and H^+-PPase retained both in the buds and the open flower petals. In contrast those proteins were absent in faded petals. PPi-and ATP-dependent H^+-transport activities in vacuolar membrane vesicles from the buds and the open flower petals were almost in the same level, although no activities in those from the faded flower petals. Therefore the vacuolar pH was not controlled by proton pumps, but might be concerning with another ion transport.

蓝色早晨荣耀的花瓣，ipomoea tricolor cv。天上的蓝色，在开花过程中将其颜色从紫色的红色变为浅蓝色。颜料是天上的蓝色花青素，分布在上和下表皮的液泡中，而不是在实质中。我们使用质子选择性微电极直接测量了活的花瓣细胞的PHV。蓝色开花的PHV为7.7，而紫色红芽的phv为6.7。这项研究提供了明确的证据，表明颜色变化是由盛开过程中的phv增加引起的。为了阐明pH的增加机制，从紫红芽的花瓣，蓝色的开花花和紫色褪色的花朵中制备了彩色原生质体，然后是提供了液泡膜。通过免疫印迹技术H^+ - ATPase和H^+ - PPase都保留在芽和开放的花瓣中。相反，这些蛋白质在褪色的花瓣中不存在。 PPI和ATP依赖性的H^+ - 来自芽和开放花瓣的液泡膜囊泡中的运输活性几乎处于相同的水平，尽管褪色的花瓣中没有活性。因此，液泡pH不受质子泵的控制，但可能与另一种离子传输有关。

项目成果

吉田 久美、近藤 忠雄: "細胞内微小電極法による花色変異の解明-アサガオの花はなぜ青色になるのか?" 電気化学および工業物理化学. 64. 1147-1152 (1996)

Kumi Yoshida、Tadao Kondo：“利用细胞内微电极方法阐明花的颜色变化 - 为什么牵牛花是蓝色的？” 64. 1147-1152 (1996)。

Yoshida, K., Kondo, T.: "Study on the mechanism of flower color variation by using proton selective micro-electrode-Why the petal of morning glory turns blue? -" Denki Kagaku. 64. 1147-1152 (1996)

吉田K.，近藤T.：“利用质子选择性微电极研究花色变化机制——牵牛花的花瓣为什么会变成蓝色？”-《电气化学》。

Yoshida, K.et al: "Structural analysis and measurement of anthocyanins from colored seed coats of Vigna,Phaseolus,and glycine legumes." Biosci.Biotech.Biochem.60. 589-593 (1996)

Yoshida, K.等人：“豆科植物、菜豆和甘氨酸豆类彩色种皮中花青素的结构分析和测量。”

Yoshida, K., Kameda, K.Kondo, T.: The Chemistry and Applications of Anthocyanins and Flavones from Perilla Leaves. in Perilla. (He-ci, Y., Kosuna, K.and Haga, M.eds.) Harwood Academic Publishers, Amsterdam, 149-169 (1997)

吉田，K.，龟田，Kondo，T.：紫苏叶花青素和黄酮的化学和应用。

Yoshida, K., Sato, Y., Okuno, R., Kameda, K., Isobe, M., Kondo, T.: "Structural analysis and measurement of anthocyanins from colored seed coats of Vigna, Phaseolus, and Glycine legumes." Biosci.Biotech.Biochem.60. 589-593 (1996)

Yoshida, K.、Sato, Y.、Okuno, R.、Kameda, K.、Isobe, M.、Kondo, T.：“对蚕豆、菜豆和大豆有色种皮中的花青素进行结构分析和测量。