Study on development and clinical application of new bactericidal-biocompatible material for conserving infected pulp.

新型杀菌生物相容性牙髓保存材料的开发及临床应用研究

• 批准号: 08407063
• 负责人: IWAKU Masaaki
• 金额: $ 20.74万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08407063/
• 关键词: Infected Pulp; Obligate Anaerove; Metoronidazol; alpha-Tricalcium phosphate; Biocompatibility; Antibacterial Drug; Hard Tissure Barrier; Pulp Conservation

This study was projected for development and clinical application of the most effective new bactericidal-biocompatible material to conserve the infected pulp tissue.Following results were obtained :1. RFLP Analysis of 16S rDNA from Pseudolamibacter alactolyticus.In this study, we examined the restriction fragment-length polymorphism(RFLP) of the 16S-rDNA amplified by poymerase chain reaction(PCR) to assess phylogenetic heterogeneity of this bacterial species. In conclusion, these data suggested that P.alactolyticus may not be a phylogenetically homogeneous species.2. Artificial Bacterial Bioflims and Confocal Laser Scanning Microscopic Analysis.To elucidate the structure and the function of biofilms, mucoid-type Pseudomonas aeruginosa organisms were cultured in broth media supplied. The biofilm structure was visualized by fluorescent staining and were exposed to ciprofloxacin.These results indicate that the confocal laser scanning microscopical analysis of artificial biofilms was useful to elucidate bacterial functions in bioflims, and may lead to new quantitative estimation of bactericidal efficacy of natibacterial drugs.3. Tooth development is regulated by epithelial-mesenchymal interactions mediated by the basement membrane. The expression and localization of laminin-5 were temporally and spatially regulated during mouse tooth development. The temporo-spatial changes in basement membrane composition might be related with tooth morphogenesis and cytodifferentiation.4. The amount of fluoride release from fluoridated restorative materials, fluoride uptake into enamel and dentine, and effect of fluoride on acid resistance of the tooth structures, were evaluated Fluoride releasing materials was effective for prevention of secondary caries.5.This work is now in progress

这项研究预计是用于开发和临床应用最有效的新型杀菌生物兼容材料来保存感染的果肉组织。在这项研究中，我们检查了通过Poymerase链反应（PCR）扩增的16S-RDNA的限制片段长度多态性（RFLP），对16S rDNA进行了RFLP分析。总之，这些数据表明，分裂性溶液可能不是系统发育均匀的物种。2。人造细菌生物膜和共聚焦激光扫描显微镜分析。为了阐明生物膜的结构和功能，在提供的肉汤培养基中培养了粘液型假单胞菌铜绿铜生物体。通过荧光染色可视化生物膜结构，并暴露于环丙沙星。这些结果表明，对人工生物膜的共聚焦激光扫描显微镜分析可用于阐明生物flim的细菌功能，并可能导致Natibibactibactibactibical ss.3的新定量估计。牙齿发育受到基底膜介导的上皮 - 间质相互作用的调节。在小鼠牙齿发育过程中，层粘连蛋白5的表达和定位在时间和空间上受到调节。基底膜组成的颞空间变化可能与牙齿的形态发生和细胞分化有关。4。评估了氟化物从氟化材料中释放的量，氟化物对搪瓷和牙本质的摄取以及氟化物对牙齿结构耐酸性的影响，对氟化物释放材料进行了评估，可有效预防次要龋齿。5。这项工作正在进行中

项目成果

Kunihiko Yoshiba: "Expression and localization of laminin-5 subunits during mouse tooth development." Developmental Dynamics. 211. 164-176 (1998)

Kunihiko Yoshiba：“小鼠牙齿发育过程中层粘连蛋白 5 亚基的表达和定位。”

Nagako Yoshiba et al: "Expression and localization of laminin-5 subunits in the mouse incisor" Cell and Tissue Research. 292巻. 143-149 (1998)

Nagako Yoshiba 等人：“小鼠门牙中层粘连蛋白 5 亚基的表达和定位”细胞和组织研究 292. 143-149 (1998)。

N.Yoshiba: "Immunolocalization of the small proteoglycan decorin in human teeth." Archs oral Biol.41(4). 351-357 (1996)

N.Yoshiba：“人类牙齿中小蛋白聚糖核心蛋白聚糖的免疫定位。”

韓 臨麟et al: "接着性コンポジットレジンにおけるフッ素含有フィラー配合ボンディング材に関する研究-フッ素徐放性,歯質への取り込みおよびその歯質耐酸性に及ぼす影響-" 日歯保存誌. 41巻. 690-697 (1998)

Lin-Lin Han 等人：“粘合复合树脂中含有含氟填料的粘合材料的研究 - 牙齿结构中氟化物的持续释放、吸收及其对牙齿结构耐酸性的影响 -”《牙科保护杂志》第 41 卷。 690 -697 (1998)

Kunihiko Yoshiba et al: "Expression and localization of laminin-5 subunits during mouse tooth development" Developmental Dynamics. 211巻. 164-176 (1998)

Kunihiko Yoshiba 等人：“小鼠牙齿发育过程中层粘连蛋白 5 亚基的表达和定位”，Developmental Dynamics，第 211 卷，164-176（1998 年）。