Bacteriological Study for Prevention of Recurrent Caries

预防复发性龋齿的细菌学研究

基本信息

批准号：
60440088
负责人：
IWAKU Masaaki
金额：
$ 11.65万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60440088/
关键词：
Recurrent caries Anaerobes Metronidazole α-TCP 嫌気性菌 Metronidazole

项目摘要

Bacteriological Study for Prevention of Recurrent Caries This study was projected to develop the most effective clinical method for preventing recurrent caries by using bactericidal material against bacteria in human carious dentin. It was clarified by Hoshino that the majority of microorganisms isolated from both shallow and deep layers of dentinal lesions are obligate anaerobes, which suggests that autimicrobial treatment directed specifically against anaerobes may be effective in preventing complications. And, it has been well known that metronidazole(MN) has a wide spectrum of bactericidal action against obligate anaerobes.Bactericidal efficacy of MN against bacteria in human carious dentin was clarified as follows:1. Bacterial recovery from human carious dentin obtained from patients was measured on BHI-Blood agar plates containing MN(10<mu>g/ml) and on those lacking MN, and the addition of MN caused more than 99% reduction of bacterial recovery.2. Overwhelming majority of bacteria in carious dentin of freshly extracted human teeth was not recovered when the lesions were covered with <alpha>-Tricalcium Phosphate <alpha>-TCP) cement containing 5% MN for 1-3 days, whereas more than 10^3 bacteria(CFU) were obtained when the lesions were covered with <alpha>-TCP cement containing no MN.3. No any bacteria were recovered from the samples of dentinal lesions which hab been covered by <alpha>-TCP cement containing MN in situ for 1 day, 28 days and/or 1 year ana/or 2 years in vivo. The bactericidal efficacy of MN in situ is clearly demonstrated, suggesting that topical application of MN is effective in caries treatment to deisinfect bacteria which remained accidentally in the smear layer and under restorations or intentionally in the deep layers of acute dentin caries of the child's permanent molar just after eruption to avoid pulp exposure, or invade newly from the tooth surfaces.

预防复发性龋齿的细菌学研究预计，通过在人类龋齿中使用针对细菌的杀菌材料来开发最有效的临床方法来预防复发性龋齿。 Hoshino澄清说，从浅层和深层牙本质病变中分离出的大多数微生物都是质感的厌氧菌，这表明特异性针对厌氧菌专门针对厌氧菌的自发治疗可能有效地预防并发症。而且，众所周知，甲硝唑（MN）具有针对强制性厌氧菌的杀菌作用广泛。在人类保育牙本中，MN对细菌的肉杆菌疗效阐明了如下：1。在含有MN的Bhi-Blood琼脂板上测量了从患者获得的人类龋齿的细菌恢复（10 <mu> g/ml），并在缺乏MN的人群中测量了MN的添加，而MN的添加导致细菌恢复降低超过99％。2。当病变被<Alpha> - 磷酸三氯磷酸三磷酸含5％MN的磷酸含量<Alpha> - 磷酸三磷酸含5％Mn时，未回收新鲜萃取的人牙齿中的绝大多数细菌，而在没有10^3细菌（CFU）的情况下，含有5％Mn的水泥，而<Alphe则覆盖了<alsn opp。没有从牙齿病变的样品中回收任何细菌，这些细菌均由<Alpha> -TCP水泥覆盖，该水泥含有Mn的原位，持续1天，28天和/或1年ANA/或2年在Vivo中。 The bactericidal efficacy of MN in situ is clearly demonstrated, suggesting that topical application of MN is effective in caries treatment to deisinfect bacteria which remained accidentally in the smear layer and under restorations or intentionally in the deep layers of acute dentin caries of the child's permanent molar just after eruption to avoid pulp exposure, or invade newly from the tooth surfaces.