E.coli Genome Project

大肠杆菌基因组计划

基本信息

批准号：
08309009
负责人：
HORIUCHI Takashi
金额：
$ 7.04万
依托单位：
(National Institute For Basic Biology) Okazaki National Research Institutes
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08309009/
关键词：
Escherichia coli genome analysis sequencing genome project データパンク WWW 塩基配列決定データバンク

项目摘要

Escherichia coli has been playing significant roles as a model organism in establishment of a number of basic concepts in molecular biology, and the huge accumulation of data has contributed to a better understanding of cellular processes of E.coli and numerous other organisms. Such being the case, completion of analysis of the E.coli genome is vital as a reference for structures and functions of genes in various organisms.The E.coli genome project in Japan was begun in 1989, the objective being to analyze the sequence of the E.coli genome from 0 minutes and in a clockwise direction. In March 1995, the DNA sequences of the 0-13 min region on the genetic map had been determined. In April 1995, we set up a new group consisting of 12 Japanese researchers to analyze the DNA sequence of the region from 13 to 69 minutes, using a new protocol. We separated the sequencing protocol into four steps ; 1) Fragment preparation, 2) Template preparation, 3) Sequencing and 4) Analysis and storage of data. Each member of the group is responsible for one or two of these steps.The sequence of the 13-69 min region was completed in January 1997 and the E.coli genome in a contiguous sequence was completed by combining our sequence data with the sequence data of the 69-100 min region reported by Blattner's group. Analysis of our sequences was published in four separate papers, registered in DDBJ and can be found on the Web. Sequence analysis of the entire E.coli genome is under way.

Escherichia Coli在建立分子生物学的许多基本概念中一直在重要的角色中发挥着重要作用，并且大量数据积累有助于更好地理解大肠杆菌和许多其他生物的细胞过程。在这种情况下，对大肠杆菌基因组的分析完成至关重要，作为各种生物体基因的结构和功能的参考。日本的大肠杆菌基因组项目始于1989年，其目的是分析从0分钟和顺时针方向分析大肠杆菌基因组的顺序。 1995年3月，已经确定了遗传图上0-13分钟区域的DNA序列。 1995年4月，我们建立了一个由12位日本研究人员组成的新小组，使用新协议分析了该地区13至69分钟的DNA序列。我们将测序协议分为四个步骤。 1）片段制备，2）模板准备，3）测序和4）数据的分析和存储。该组的每个成员都负责其中一两个步骤。1997年1月完成了13-69分钟区域的序列，并通过将我们的序列数据与Blattner组报告的69-100 min区域的序列数据相结合，从而完成了连续序列中的大肠杆菌基因组。我们序列的分析发表在DDBJ中注册的四个单独的论文中，可以在网络上找到。整个大肠杆菌基因组的序列分析正在进行中。