STUDY ON THREE DIMENTIONAL CULTURE OF REPITONAL RESIDENT CELLS(AETIFICIAL PERITONEUM)

腹膜驻留细胞三维培养的研究

• 批准号: 11694181
• 负责人: HORIUCHI Takashi
• 金额: $ 6.14万
• 依托单位: UNIVERSITY OF EAST ASIA
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11694181/
• 关键词: 腹膜透析; 腹膜硬化症; 細胞培養; 三次元培養; 画像解析; 腹膜修復; 細胞増殖; 細胞遊走; サイトカイン; RT-PCR; VEGF; TGF-β1

Extracellular matrices layered with collagen and hyaluronan was formed in the Transwell (Corstar ; 0.3 μm pore size) and utilized for a solute-transfer experiment. Solutes with small molecular weight, except for urea, permeate these layers depending on concentration of both collagen and hyaluronan concentrations.In addition to solute-transfer experiments, a possible application of 3D model is a wound-healing study. To establish this experimental set-up, defoliation of mesothelial layer was made by a droplet of IN NaOH, and then microscopically observed by CCD camera. Digital images were aquired every 30 minutes until remesothelialization was completed. These date were then analysed to calculate cell size, rate of remesothelialization. 30〜4Q% increase of cell size were observed in comparison between pre and post healing. Digital video representation demonstrates cell migration prior to cell proliferation during wound healing on the monolayered culture of human peritoneal mesothelial cell.In conclusion 3D model of the peritoneum could be used for a various type of in vitro study such as solute-transfer experiment and wound healing experiment to elucidate fibrotic process of the peritoneum due to peritoneal dialysis procedure.

在Transwell（孔径为0.3μm）中形成了带有胶原蛋白和透明质酸的细胞外基质，除尿素外，除尿素外，还渗透到胶原蛋白和透明质酸和透明质酸浓度的情况下，渗透到这些层除外，还渗透到这些层。 - 在NaOH中的一滴液滴使间皮层变形，并且CCD摄像头观察到的微观图像。在人腹膜间皮的单层curt裂之前，细胞在细胞上迁移，可以将ITONEUM用于各种类型的体外研究，例如溶质转移经验和伤口愈合实验，以eluciDidide filedidide filediDide filedideide filetideide filetideide filetiDide filetideide fiftere fif。透析程序。

项目成果

堀内孝: "腹膜硬化"CTPD. 19(3). 6-7 (2001)

Takashi Horiuchi：“腹膜硬化”CTPD 19(3) (2001)。

堀内 孝, その他: "培養細胞の障害修復過程の画像解析システムの開発"腎と透析. (2002)

Takashi Horiuchi 等人：“用于培养细胞修复过程的图像分析系统的开发”《肾脏与透析》（2002 年）。

Horiuchi, t: "kinetics in CAPD"Therapeutics & Engineering. 11(4). 630-633 (1999)

Horiuchi, t：“CAPD 动力学”

Horiuchi, T.Nagasawa, K. et al: "Effect of glucose degradation products (GDP) and pH in peritoneal dialysate on proliferation of rat peritoneal mesothelial cell and fibroblast"J Am Soc Nephrol. 11. 209A (2000)

Horiuchi, T.Nagasawa, K. 等人：“腹膜透析液中葡萄糖降解产物 (GDP) 和 pH 对大鼠腹膜间皮细胞和成纤维细胞增殖的影响”J Am Soc Nephrol。

Horiuchi T. et al.: "Expression of transforming growth factor (TGF-β1) mRNA and contractility of extracellular"Clin Exp Nephrol. 4. 105-113 (2000)

Horiuchi T.等：“转化生长因子(TGF-β1) mRNA的表达和细胞外的收缩性”Clin Exp Nephrol。 4. 105-113 (2000)