Functional Genomics

功能基因组学

• 批准号: 08283105
• 负责人: KOHARA Yuji
• 金额: $ 344.26万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08283105/
• 关键词: gene expression; FST; RNAi; full-length cDNA; expression pattern; phenotype; gene disruption; protein-protein interaction; 遺伝子発現プロファイル; ボディマップ; ATAC-PCR; 遺伝子発現パターン; 酵母two-hybrid法; cDNA; 線虫; 出芽酵母; 枯草菌; ディファレンシャルディスプレイ

Y. Kohara carried out the systematic analysis of expression patterns of 7000 genes along the time course of development of the nematode C. elegans, and performed the clustering analysis to identify the cis-elements of various regulatory regions. K. Okubo revealed the structure of human transcriptome through the construction of the database named BodyMap which consisted of the expression patterns with respect to anatomy of some 20,000 human genes. S. Sugano developed the Oligo-capping method aiming at constructing full-length enriched cDNA libraries and applied it to the systematic analysis of the transcription start sites in the human genome. T. Itoh established a comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast S, cerevisiae proteins, and predicted several gene cascades base on the identified interactions. N. Ogasawara and collaborators carried out functional analysis of B. subtilis genome through the disruption and phenotype analysis on 2674 genes as a Japan-Europe joint project. K. Kato developed a highly sensitive method named Adapter-tagged Competitive PCR and applied it to gene expression profiling of mouse postnatal cerebellar development. Other projects were also performed as follows. E. Takahashi performed functional analysis of 162 human genes. T. Aigaki generated 2000 GS (gene-search) strains of D. melanogaster to screen various genes. S. Minani developed a highly effective method to generate deletion mutants in C. elegans. A. Sugimoto improved the soaking RNAi method and performed the phenotype analysis of 2500 C. elegans genes. M. Maeda and her collaborators carried out cDNA project of the slime mold D. discoideum. Y. Murakami carried out expression profiling of the all genes of the chromosome VI of S. cerevisiae.

Y. Kohara对线虫发育过程中7000个基因的表达模式进行了系统分析，并进行聚类分析以鉴定各个调控区域的顺式元件。 K. Okubo 通过构建名为 BodyMap 的数据库揭示了人类转录组的结构，该数据库由约 20,000 个人类基因的解剖表达模式组成。 S. Sugano 开发了旨在构建全长富集 cDNA 文库的 Oligo-capping 方法，并将其应用于人类基因组转录起始位点的系统分析。 T. Itoh 建立了一个综合系统来检查酵母 S、酿酒酵母蛋白质之间所有可能组合中的两种杂交相互作用，并根据已识别的相互作用预测了几种基因级联。 N. Ogasawara 和合作者作为日欧联合项目，通过对 2674 个基因进行破坏和表型分析，对枯草芽孢杆菌基因组进行了功能分析。 K. Kato 开发了一种名为“接头标记竞争性 PCR”的高灵敏度方法，并将其应用于小鼠出生后小脑发育的基因表达谱分析。其他项目也进行如下。 E. Takahashi 对 162 个人类基因进行了功能分析。 T. Aigaki 生成了 2000 个 GS（基因搜索）黑腹果蝇菌株来筛选各种基因。 S. Minani 开发了一种高效的方法来在线虫中产生缺失突变体。 A. Sugimoto改进了浸泡RNAi方法，对2500个线虫基因进行了表型分析。 M. Maeda 和她的合作者开展了粘菌 D. discoideum 的 cDNA 项目。 Y. Murakami 对酿酒酵母 VI 号染色体的所有基因进行了表达谱分析。

项目成果

T.Saito: "A second functional delta 5 desaturase in the cellular slime mold Dictyostelium discoideum"Eur. J. Biochem.. 267. 1813-1818 (2000)

T.Saito：“细胞粘菌盘基网柄菌中的第二种功能性 Delta 5 去饱和酶”Eur。

Yu Y: "Human papillomavirus type 33 DNA in breast cancer in chinase"Breast Cancer. 7(1). 33-36 (2000)

于Y：“中国乳腺癌中的人乳头瘤病毒33型DNA”乳腺癌。

Osada, N.: "Sequence analysis, geneexpression, and chromosomal assignment of mouse Borg4 gene and its human orthologue"J. Hum. Genet.. 45. 374-377 (2000)

Osada, N.：“小鼠 Borg4 基因及其人类直系同源物的序列分析、基因表达和染色体分配”J.

Toba, G.: "Disruption of the Microsomal glutathione S-transferase-like gene reduces life span of Drosophila melanogaster"Gene. 253. 179-187 (2000)

Toba, G.：“微粒体谷胱甘肽 S-转移酶样基因的破坏会缩短果蝇的寿命”基因。

B.S.Mitra: "Loss of a member of the aquaporin gene family, aqpA affects spore dormancy in Dictyostelium"Gene. 251. 131-139 (2000)

B.S.Mitra：“水通道蛋白基因家族成员 aqpA 的丢失会影响盘基网柄菌的孢子休眠”基因。