Genome science towards the understanding of life

基因组科学有助于理解生命

• 批准号: 12201001
• 负责人: KOHARA Yuji
• 金额: $ 2079.17万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12201001/
• 关键词: Genome Sequencing; EST; Resources; Outreach; モデル生物; 計算機モデル化; 遺伝子機能; 脊椎動物進化; 比較ゲノム; 霊長類ゲノム; 発生進化; アウトリーチ活動; 産学連携; ヒト多型タイピング; ESTシーケンシング; 多型タイピング; マイクロサテライト; SNPs; 医科学倫理問題

As the managing group of the 4 areas (this area and the other 3 genome areas), it performed the following activities;1) Coordination among this 4 areas2) Research resources; The DNA sequencing committee has performed genome sequencing of red algae (Cyanidioschyzon merolae), Ascidian (Ciona intestinalis), Y chromosome of liverwort (Marchantia polymorpha L), Medaka fish (Oryzias latipes) and wild mouse derived strain MSM by the whole genome shotgun strategy together with the end sequencing of fosmid and BAC clones. It also performed EST sequencing of nematode worms (Caenorhabditis elegans and Diploscapter sp.), Ascidian (Ciona intestinalis, Ciona savignyo and Halocynthia roretzi), Hagfish (Eptatretus burgeri), Medaka fish (Oryzias latipes), ciclid fish (Haplochromis chilotes), loach (Misgurnus anguillicaudatus), frog (Xenopus laevies), slime mold (Dictyostelium discodeum), red algae (Cyanidioschyzon merolae), moss (Physcomitrella patens subsp. patens), barley, wheat (Triticim aestivum cv. Chinese Spring), Japanese morning glory. The resource committee distributed the clones analyzed above to all over the world.3) Outreach; The publicity committee provided information on the 4 areas to the public and research community. The social relationship committee held a social event named "Genome Square" altogether 8 times (in Tokyo, Kyoto and Fukuoka) during last 3 years, in which the research groups in the 4 areas presented their posters and research materials to communicate with the general public. The committee also discussed on the ethics issue in genome medicine.

作为这 4 个领域（该领域和其他 3 个基因组领域）的管理小组，它开展了以下活动；1) 这 4 个领域之间的协调；2) 研究资源； DNA测序委员会采用全基因组鸟枪法对红藻（Cyanidioschyzon merolae）、海鞘（Ciona Enteris）、地钱（Marchantia polymorpha L）的Y染色体、青鳉鱼（Oryzias latipes）和野生小鼠源品系MSM进行了全基因组鸟枪法测序。以及 fosmid 和 BAC 克隆的末端测序。它还对线虫（Caenorhabditis elegans 和 Diploscapter sp.）、海鞘（Ciona Enteris、Ciona savignyo 和 Halocynthia roretzi）、盲鳗（Eptatretus Burgeri）、青鳉鱼（Oryzias latipes）、慈鲷（Haplochromis chilotes）、泥鳅进行了 EST 测序（泥鳅anguillicaudatus）、青蛙（Xenopus laevies）、粘菌（Dictyostelium discodeum）、红藻（Cyanidioschyzon merolae）、苔藓（Physcomitrella patens subsp. patens）、大麦、小麦（Triticim aestivum cv. Chinese Spring）、日本牵牛花。资源委员会将上述分析的克隆分发到世界各地。3）外展；宣传委员会向公众和研究界提供了这4个领域的信息。社会关系委员会近3年共举办8次“基因组广场”社交活动（东京、京都、福冈），4个地区的研究小组展示海报和研究资料，与公众进行交流。委员会还讨论了基因组医学的伦理问题。

项目成果

Ohta, N.: "Complete sequence and analysis of the plastid genome of the unicellular red alga CyanidiosChyzon merolae"DNA Research. (in press). (2003)

Ohta, N.：“单细胞红藻 CyanidiosChyzon merolae 质体基因组的完整序列和分析”DNA 研究。

Nucleotide sequence comparison of a chromosome rearrangement on the human chromosome 12 and corresponding ape chromosomes

人类12号染色体和相应猿染色体上染色体重排的核苷酸序列比较

• 发表时间: 2005
• 期刊: Cytogenetic and Genome Research 108(1-3)
• 作者: Teiya Kijimoto;et al.;Shimada M et al.
• 通讯作者: Shimada M et al.

Tetsuya Muramoto: "Construction of a gamete-enriched gene pool and RNAi-mediated functional analysis in Dictyostelium discoideum."Mechanisms of Development. 120. 965-975 (2003)

Tetsuya Muramoto：“盘基网柄菌中配子富集基因库的构建和 RNAi 介导的功能分析。”发育机制。

Reboul,J.: "Open-reading-frame sequence tage (OSTs) support the existence of at least 17, 300 genes in C.elegans."Nature Genetics. 27. 332-336 (2001)

Reboul, J.：“开放阅读框序列标签 (OST) 支持线虫中至少存在 17、300 个基因。”《自然遗传学》。

Medaka genomics : a bridge between mutant phenotypes and gene function.

青鳉基因组学：突变表型和基因功能之间的桥梁。

• 发表时间: 2004
• 期刊: Mechanisms of Development 121
• 作者: Kiyoshi Naruse;et al.
• 通讯作者: et al.