Biological significance of Prolinerich antimicrobial peptide

富含脯氨酸抗菌肽的生物学意义

• 批准号: 08044228
• 负责人: KOHGO Yutaka
• 金额: $ 2.05万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044228/
• 关键词: antimicrobial peptide; PR-39; syndecan; metastasis; cytoskeletone; ras sinal; 内因性抗菌ペプチド; antimicrobial peptide; PR-39; syndecan; metastasis; cytoskeletone; ras sinal; 内因性抗菌ペプチド

We clarified several important points about syndecans and PR-39 through colaborations and discussions with Dr.Gallo as shown below.1.We made polyclonal antibody against PR-39 afer immunization of rabbits using N-terminal 15 aminoacids oigopeptides.2.We observed the band at the size of approximately 5 kDa as expected size of PR-39 mature protein afer immunoprecipitation of human leukocytes protein using PR-39 polyclonal antibody. However, we have not cloned the protein which might be human counterpart of pig PR-39.3.PR-39 has a possible function which can bind to Src homology 3 domain, becaus PR-39 has five repeats of proline rich motif that has been reported to bind SH3 domain as like a Sos protein which can bind to Grb2 protein. Recombinant SH3 domain of Src gene can actually bind to PR-39 oligopeptides by the study of immunopregipitation.4.PR-39 gene transfection into human hepatoma cells showed induction of syndecan-1 expression, suppression of invasive activity and cell morphological change. These results might be due to the binding ability of PR-39 into SH3 domain.5.PR-39 transfectans excreted PR-39 protein into conditioning medium.6.PR-39 gene transfection into ras transformants also showed morphological change and decrease of proliferation rate.

我们通过与Gallo博士进行的合同和讨论阐明了有关Syndecans和Pr-39的几个重要点。1。我们对PR-39对兔子进行多克隆抗体的抗体，使用N末端15氨基酸氨基酸酯氨基酸酯oigopeptids oigopeptides oigopeptides。白细胞蛋白使用PR-39多克隆抗体。但是，我们尚未将蛋白质克隆起来，该蛋白可能是PIG PR-39.3.3.3.39的人类可能与SRC同源性3结构结合的功能，因为PR-39具有五个重复脯氨酸富基序的重复，该基序已据报道与SOS蛋白一样结合SH3结构域，就像SOS蛋白一样可以与GRB2蛋白结合。 SRC基因的重组SH3结构域实际上可以通过研究免疫调节来与PR-39寡肽结合。4.PR-39基因转染到人肝癌细胞中显示出促辛代克-1表达，抑制侵入性活性和细胞形态学变化。这些结果可能是由于PR-39到SH3结构域的结合能力。5.PR-39经染料将PR-39蛋白排泄到条件培养基中。6.PR-39基因转染到Ras转化体中也显示出形态变化和增殖率的降低。