Genes those regulate internal osmolality

调节内部渗透压的基因

• 批准号: 07672344
• 负责人: NAKAMURA Tatsunosuke
• 金额: $ 1.02万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672344/
• 关键词: trkA; mscL; nhaB; osmolality; gene; proC

(1) trkAv and neighboring genes.We clone K^+ uptake genes, trkAv and trkHv, from marine bacterium, Vibrio alginolyticus, those functionally complement K^+ uptake deficient E.coli strains. K^+ uptake activities of those strains with trkAv and/or trkHv genes are determined. Expression of TrkAv and TrkHv were examined also.(2) mscL gene.We examined the activity of MscL in Vibrio alginolyticus.(3) nhaBv gene.We cloned nhaBv, the second Na^+/H^+ antiporter, gene from V.alginolyticus. Topological experiments were done by phoA gene fusion method. It was appeared that NhaBv has odd number transmembrane segments.

（1）Trkav和相邻基因。我们克隆K^+摄取基因，Trkav和TrkHv，来自海洋细菌，弧菌al藻溶解度，这些基因在功能上补充了K^+摄取不足的E.coli菌株。确定了这些菌株的K^+摄取活性和/或TRKHV基因。 （2）MSCL基因也检查了TRKAV和TRKHV的表达。我们检查了MSCL在Vibrio Alginoloselyticus中的活性。（3）NHABV基因。我们克隆了Nahhabv，第二个Na^+/H^+反orter，来自V。藻素溶解度。拓扑实验是通过Phoa基因融合法完成的。似乎NHABV具有奇数跨膜段。

项目成果

Tatsunoske Nakamura et al.: "Cloning and sequencing of novel genes from Vibrio alginolyticus" Biochemica et Biophysica Acta.vol 1277. 201-208 (1996)

Tatsunoske Nakamura 等：“来自溶藻弧菌的新基因的克隆和测序”Biochemica et Biophysicala Acta.vol 1277. 201-208 (1996)

Tatsunosuke Nakamura et al.: "Cloning and seqnencing of novelgenes from Vibrio alginolyticns" Biochemica et Biophysica Acta. 1277. 201-208 (1996)

Tatsunosuke Nakamura 等人：“溶藻弧菌新基因的克隆和测序”《生物化学与生物物理学学报》。

Tatsunosuke Nakamura et al.: "Cloning and seqnencing of the nhaB gene encoding an Na^+/H^+ antipester." Biochemica et Biophysica Acta. 1275. 157-160 (1996)

Tatsunosuke Nakamura 等人：“编码 Na^ /H^ 抗虫剂的 nhaB 基因的克隆和测序。”

Tatsunosuke Nakamura et al.: "Cloning and sequencing of the nhaB gene encoding an Na^+/H^+ antiporter" Biochimaca et Biophysica Acta. 1275. 157-160 (1996)

Tatsunosuke Nakamura 等：“编码 Na+/H+ 反向转运蛋白的 nhaB 基因的克隆和测序”Biochimaca et Biophysical Acta。

Tatsunosuke Nakamura et al.: "Cloning and sequencing of novel genes from Vibrio alginolyticus" Biochimica et Biophysica Acta. 1277. 201-208 (1996)

Tatsunosuke Nakamura 等人：“溶藻弧菌新基因的克隆和测序”Biochimica et Biophysicala Acta。