The study of inflammatory and defensive mechanisms in otitis media with effusion.

渗出性中耳炎炎症和防御机制的研究。

基本信息

批准号：
07671872
负责人：
HIMI Tetsuo
金额：
$ 1.41万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671872/
关键词：
Otitis media Cytokine Chemokine Neutrophil Adhesion molecules 走化因子

项目摘要

In this project, in vivo induction and regulation of chemokine, cytokines and cell adhsion molecules in the middle ear were investigated using topical inoculation with KLH,lopopolysaccharide (LPS) and Lipoteichoic acid (LTA) in rat model. The expressions of mRNA in the middle ear mucosa was also examined.At first, we examined the expression of cell adhesion molecules (CAM) in immune-mediated otitis media in the rat, as well as the regulation of these CAM in peripheral blood polymorphonuclear leukocytes (PMN) and lymphocytes upon exposure to middle ear effusion (MEE). The expression of CAM was important for the initiation of otitis media. Moreover, it ws thought that the interaction between the infiltrated PMN and MEE may modify the CAM's expression during the inflammatory process in the middle ear cavity.Chemokine possesses chemotactic-activating properties toward neutrophils, and may contribute to the pathogenesis of middle ear inflammations. We investigated the production of chemokine in middle ear lavage and that gene expression in the middle mucosa using topical inoculation with LPS and LTA in the rat in vivo model. In contrtast to LPS as gram-negative endotoxin, lipoteichoic acid (LTA) is present within this layr of LPS as gram-negative endotoxin, lipoteichoic acid (LTA) is present within this layr of mo gram-positive bacteria. LTA has been shown to play a sigificant role in the initiation and progression of bacterial infection. Chemokine in middle ear lavage showed time-and dose-dependent production under LPS or LTA stimulation. At the time of peak production, the expression of cytokines and chemokine mRNA,evaluated using polymerase chain reaction, was considerable in the middle ear mucosa.This investigation of the characteristics of proinflammatory cytokines in the middle ear cavity using rat in vivo model has extended the functional concept of cytokines at the initial stage otitis media.

在这个项目中，使用KLH，洛普多糖（LPS）和脂肪酸（LTA）在大鼠模型中研究了中耳中趋化因子，细胞因子和细胞依性分子的体内诱导和调节。还检查了中耳粘膜中mRNA的表达。首先，我们检查了大鼠免疫介导的耳鼻炎培养基中细胞粘附分子（CAM）的表达，以及在外周血多态性白血细胞（PMN）和淋巴细胞中对这些CAM的调节（在膨胀到中间）的调节（中间）。 CAM的表达对于开始中耳炎培养基很重要。此外，它认为，浸润的PMN和MEE之间的相互作用可能会在中耳腔中炎症过程中修改CAM的表达。Femokine具有趋化性激活的特性，倾向于中性粒细胞，并可能有助于中耳炎症的病原体。我们利用大鼠在体内模型中使用LPS和LTA的局部接种，研究了中耳灌洗中趋化因子的产生，并在中部粘膜中的基因表达产生。与LPS作为革兰氏阴性内毒素相反，在该LYR的LPS中存在脂甲酸（LTA），作为革兰氏阴性内毒素，Lipoteichoic Acid（LTA）存在于MO革兰氏阳性细菌的这一中。 LTA已显示在细菌感染的起始和进展中起着重要作用。中耳灌洗中的趋化因子在LPS或LTA刺激下显示时间依赖于剂量的产生。在产生峰值时，使用聚合酶链反应评估的细胞因子和趋化因子mRNA的表达在中耳粘膜中相当大。此研究研究了使用体内大鼠模型中中耳腔中促炎性细胞因子在初始阶段卵巢膜媒体上的细胞因子函数概念的特征。