GENETIC STUDY ON ALLOTETRAPLOIDY GOLDFISH AND CRUSSIAN CARP BY MATING EXPERIMENTS

异源四倍体金鱼和鲫鱼的交配遗传研究

基本信息

批准号：
07660232
负责人：
KIJIMA Akihiro
金额：
$ 1.34万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660232/
关键词：
MATING EXPERIMENT GENETIC CONTROL ISOZYMES GOLDFISH CRUSSIAN CARP GENETIC MARKER キンブナ異質4倍体 mtDNA

项目摘要

Since goldfish and crussian carp would have been evolved from allo-tetraploid origin, isozyme patterns appeared with complexities and genetic control of them could not be clarified yet. Then genetic analyzes for inter- and intra-specific relationship has not been carried out deeply, yet. In the present study, convenient usable genetic markers for population study are chosen and the genetic control of chosen markers has been analyzed by mating experiments. As the results, (1) Nuclear DNA and mitochondrial DNA are usable genetic markers for individual and population study, but not convenient compared with isozymes. (2) Clear bands of isozymes in 10 enzymes appeared by starch gel electrophoresis, easily. (3) Genetic control of 21 out of 26 isozymic loci control for 10 enzymes has been demonstrated in gold fish and crussian carp by 3 and 11 combinations of pair maiting experiments, respectively. (4) But further combination of mating are needed for clarify the genetic control of isozymes in Gpi-2 and Ldh-1,2,3,4 loci. (5) Genetic variability is higher in natural population of crussian carp than in artificial strains of goldfish, but not fixed in any strain of goldfish which has been selected strictly. (6) Levels of genetic differentiation are estimated as local race level within strains of goldfish and within population of natural crussian carp, and as subspecies level between goldfish and natural crussian carp.

由于金鱼和鲫鱼是从异源四倍体起源进化而来的，同工酶模式显得很复杂，而且它们的遗传控制尚无法阐明。种间和种内关系的遗传分析尚未深入开展。在本研究中，选择了方便用于群体研究的遗传标记，并通过交配实验分析了所选标记的遗传控制。结果表明：（1）核DNA和线粒体DNA是个体和群体研究中可用的遗传标记，但与同工酶相比并不方便。 (2)淀粉凝胶电泳显示10种酶的同工酶条带清晰、容易。 (3) 通过3个和11个配对交配实验的组合，分别在金鱼和鲫鱼中证实了对10种酶的26个同工酶位点控制中的21个的遗传控制。 (4)但需要进一步的交配组合来阐明Gpi-2和Ldh-1,2,3,4位点同工酶的遗传控制。 (5)自然种群鲫鱼的遗传变异性高于人工金鱼品系，但在经过严格筛选的金鱼品系中遗传变异性并不固定。 (6) 遗传分化水平估计为金鱼品系和天然鲫鱼种群内的地方种族水平，以及金鱼和天然鲫鱼之间的亚种水平。