Breeding structure in fish population by RFLP analysis of mitochondrial DNA

通过线粒体 DNA 的 RFLP 分析确定鱼类种群的繁殖结构

基本信息

批准号：
02660186
负责人：
KIJIMA Akihiro
金额：
$ 1.41万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

The RFLP(restriction fragment length polymorphism)analysis of the mitochondrial DNA(MTDNA)genome is a suitable method for estimating the number of maternal lines in cultured stocks because : maternal inheritance, it is a nonrecombining genome(apploximately 16-18Kbp)in animals, the number of haplotypes represents the number of maternal lines directly and so on.In the present study, I : (l)modification of the method of rapid isolation of mtDNA from carp and masu salmon complex is successfully carried out by alkaline treatment with glassailk. (2)The 5 haplotypes were detected in the two cultured stocks of carp by using 15 kinds of 6-base recognizing restriction endonucleases. (3)Restriction maps of the haplotypes were made up by double-digestion of endonucleases and then the 33 restriction sites were defined in the carp. (4)From the results of restriction naps, the variation of mtDNA is considered to be occurred by the simple substitution of bases. II : Based on the results of RFLP analysis of mtDNA using six 6-base recognized restriction endonuclease in the cultured stocks of masu salmon complex, (5)13 mtDNA haplotypes were observed among 59 individuals of the 4 stocks. (6)The nucleotide diversity within the non-selected stocks ranged from 0.00170 to 0.00253, while no variation was observed in selected amago-parr stock. (7)The results suggest that the amago-parr stock consists of one maternal line while the other stocks consist of several maternal lines.

线粒体DNA（mtDNA）基因组的RFLP（限制片段长度多态性）分析是一种适合估计培养股票中母性线数量的合适方法，因为：母体遗传，它是一种非混合基因组（Applox opplox opplox，大约16-18kbp）（Applox offlox offlox of applox offlox，大约16-18kbp）的动物中的数量是so的数量，是so的数量。（l）通过碱性用玻璃编处理，成功地从鲤鱼和Masu鲑鱼复合物中快速分离mtDNA的方法。（2）通过使用15种识别限制性核酸内切酶的6台鲤鱼培养物库存中检测到5种单倍型。（3）单倍型的限制图是通过核酸内切酶的两倍消化组成的，然后在鲤鱼中定义了33个限制位点。（4）从限制性的结果来看，mtDNA的变化被认为是通过简单的碱基替代而发生的。 II：基于MASU鲑鱼复合物培养的库存中的六个基准识别限制性核酸酶对MTDNA的RFLP分析结果，（5）在4个股票的59个个体中观察到13 mtDNA单倍型。（6）非选择股票内的核苷酸多样性范围为0.00170至0.00253，而在某些Amago-Parr股票中未观察到变化。（7）结果表明，Amago-Parr股票由一条产妇线组成，而其他股票由几种母线组成。