Breeding structure in fish population by RFLP analysis of mitochondrial DNA

通过线粒体 DNA 的 RFLP 分析确定鱼类种群的繁殖结构

基本信息

批准号：
02660186
负责人：
KIJIMA Akihiro
金额：
$ 1.41万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

The RFLP(restriction fragment length polymorphism)analysis of the mitochondrial DNA(MTDNA)genome is a suitable method for estimating the number of maternal lines in cultured stocks because : maternal inheritance, it is a nonrecombining genome(apploximately 16-18Kbp)in animals, the number of haplotypes represents the number of maternal lines directly and so on.In the present study, I : (l)modification of the method of rapid isolation of mtDNA from carp and masu salmon complex is successfully carried out by alkaline treatment with glassailk. (2)The 5 haplotypes were detected in the two cultured stocks of carp by using 15 kinds of 6-base recognizing restriction endonucleases. (3)Restriction maps of the haplotypes were made up by double-digestion of endonucleases and then the 33 restriction sites were defined in the carp. (4)From the results of restriction naps, the variation of mtDNA is considered to be occurred by the simple substitution of bases. II : Based on the results of RFLP analysis of mtDNA using six 6-base recognized restriction endonuclease in the cultured stocks of masu salmon complex, (5)13 mtDNA haplotypes were observed among 59 individuals of the 4 stocks. (6)The nucleotide diversity within the non-selected stocks ranged from 0.00170 to 0.00253, while no variation was observed in selected amago-parr stock. (7)The results suggest that the amago-parr stock consists of one maternal line while the other stocks consist of several maternal lines.

线粒体DNA（MTDNA）基因组的RFLP（限制性片段长度多态性）分析是估计养殖种群中母系数量的合适方法，因为：母系遗传，它是动物中的非重组基因组（大约16-18Kbp），单倍型的数量直接代表母系的数量等等。在本研究中，我：（l）mtDNA快速分离方法的改进鲤鱼和马苏鲑鱼复合物通过用 glassailk 进行碱处理成功进行。 (2)利用15种6碱基限制性内切酶检测了2个鲤鱼养殖种群的5个单倍型。 (3)通过内切酶双酶切绘制单倍型酶切图谱，并在鲤鱼中确定33个酶切位点。 (4)从限制性nap的结果来看，mtDNA的变异被认为是由碱基的简单取代引起的。 II：基于使用6个6碱基识别的限制性内切酶对masu鲑鱼复合体的养殖种群中的mtDNA进行RFLP分析的结果，(5)在4个种群的59个体中观察到13种mtDNA单倍型。 (6)未选择的种群中的核苷酸多样性范围为0.00170至0.00253，而选定的amago-parr种群中未观察到变化。 (7)结果表明，amago-parr种群由一个母系组成，而其他种群由多个母系组成。