Genetic analysis in the family Cyprinidae base on isozymes and mtDNA

基于同工酶和mtDNA的鲤科遗传分析

基本信息

批准号：
63560179
负责人：
KIJIMA Akihiro
金额：
$ 1.47万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

There are many kind of fish species in the family cyprinidae, and these species evolved from allo-tetraploid fish species. Then, they could be constructed a complex genetic feature and genetic structure. Isozymic analyses are very useful tool for analyzing a population structure, genetic divergence and evolutionary feature, but they could analyze a long generations.Mitochondrial DNA (mtDNA) has been expected to use as sensitive genetic markers for fish genetics and fishery management. However, in order to apply it for actual population study, a lot of issues should be resolved.The aims in the present study are (1) establishment an elementary method for purifying mtDNA in freshwater fish, (2) Clarifying a value of restriction fragment length polymorphism in carp by use a genetic marker of mtDNA, and (3) identification of species of crucian carp inhabited in Tohoku area in Japan.As the results, the elementary method for purifying mtDNA could be established based on chemical methods (alkarine treatment and non-idet P-40). Next, based on that method, restriction fragment length polymorphisms were observed in the four restriction enzymes, and then the 4 maternal strains are estimated in carp inhabited in a certain pond. Furthermore, the three sub species of crucian carp have existed in the Tohoku area, and genetically divergent populations have been inhabited and brood in a pond. However, mtDNA variant patterns were common in both populations.

Cyprinidae家族中有许多鱼类，这些物种是从异种四倍体鱼类中演变而来的。然后，可以构建它们的复杂遗传特征和遗传结构。同学分析是分析种群结构，遗传差异和进化特征的非常有用的工具，但是它们可以分析长代。MINECHOCHRIALDNA（MTDNA）有望用作鱼类遗传学和渔业管理的敏感遗传标记。 However, in order to apply it for actual population study, a lot of issues should be resolved.The aims in the present study are (1) establishment an elementary method for purifying mtDNA in freshwater fish, (2) Clarifying a value of restriction fragment length polymorphism in carp by use a genetic marker of mtDNA, and (3) identification of species of crucian carp inhabited in Tohoku area in Japan.As the results, the可以根据化学方法（烷烃处理和非IDET P-40）建立纯化mtDNA的基本方法。接下来，基于该方法，在四种限制酶中观察到限制片段长度多态性，然后在居住在某个池塘中的鲤鱼中估计4种母体菌株。此外，在Tohoku地区存在三种克鲁克鲤鱼的三种种类，遗传上的种群被居住在池塘中。但是，mtDNA变体模式在两个人群中都是常见的。