Genetic analysis in the family Cyprinidae base on isozymes and mtDNA

基于同工酶和mtDNA的鲤科遗传分析

基本信息

批准号：
63560179
负责人：
KIJIMA Akihiro
金额：
$ 1.47万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

There are many kind of fish species in the family cyprinidae, and these species evolved from allo-tetraploid fish species. Then, they could be constructed a complex genetic feature and genetic structure. Isozymic analyses are very useful tool for analyzing a population structure, genetic divergence and evolutionary feature, but they could analyze a long generations.Mitochondrial DNA (mtDNA) has been expected to use as sensitive genetic markers for fish genetics and fishery management. However, in order to apply it for actual population study, a lot of issues should be resolved.The aims in the present study are (1) establishment an elementary method for purifying mtDNA in freshwater fish, (2) Clarifying a value of restriction fragment length polymorphism in carp by use a genetic marker of mtDNA, and (3) identification of species of crucian carp inhabited in Tohoku area in Japan.As the results, the elementary method for purifying mtDNA could be established based on chemical methods (alkarine treatment and non-idet P-40). Next, based on that method, restriction fragment length polymorphisms were observed in the four restriction enzymes, and then the 4 maternal strains are estimated in carp inhabited in a certain pond. Furthermore, the three sub species of crucian carp have existed in the Tohoku area, and genetically divergent populations have been inhabited and brood in a pond. However, mtDNA variant patterns were common in both populations.

鲤科鱼类种类繁多，都是由异源四倍体鱼类进化而来。然后，它们可以构建复杂的遗传特征和遗传结构。同工酶分析是分析种群结构、遗传分化和进化特征的非常有用的工具，但它们可以分析很长的世代。线粒体DNA (mtDNA) 有望用作鱼类遗传学和渔业管理的敏感遗传标记。然而，为了将其应用于实际群体研究，还需要解决许多问题。本研究的目的是（1）建立淡水鱼线粒体DNA纯化的基本方法，（2）阐明限制性片段的值利用mtDNA遗传标记对鲤鱼的长度多态性进行研究，以及（3）对日本东北地区栖息的鲫鱼进行物种鉴定。由此，可以建立纯化mtDNA的基本方法。化学方法（碱处理和非 idet P-40）。接下来，基于该方法，观察4种限制性内切酶的限制性片段长度多态性，然后估计某池塘中栖息的鲤鱼的4种母本品系。此外，东北地区已经存在鲫鱼的三个亚种，并且在池塘中栖息和孵化着基因不同的种群。然而，线粒体DNA变异模式在这两个人群中都很常见。