Genetic analysis in the family Cyprinidae base on isozymes and mtDNA
基于同工酶和mtDNA的鲤科遗传分析
基本信息
- 批准号:63560179
- 负责人:
- 金额:$ 1.47万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1988
- 资助国家:日本
- 起止时间:1988 至 1989
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
There are many kind of fish species in the family cyprinidae, and these species evolved from allo-tetraploid fish species. Then, they could be constructed a complex genetic feature and genetic structure. Isozymic analyses are very useful tool for analyzing a population structure, genetic divergence and evolutionary feature, but they could analyze a long generations.Mitochondrial DNA (mtDNA) has been expected to use as sensitive genetic markers for fish genetics and fishery management. However, in order to apply it for actual population study, a lot of issues should be resolved.The aims in the present study are (1) establishment an elementary method for purifying mtDNA in freshwater fish, (2) Clarifying a value of restriction fragment length polymorphism in carp by use a genetic marker of mtDNA, and (3) identification of species of crucian carp inhabited in Tohoku area in Japan.As the results, the elementary method for purifying mtDNA could be established based on chemical methods (alkarine treatment and non-idet P-40). Next, based on that method, restriction fragment length polymorphisms were observed in the four restriction enzymes, and then the 4 maternal strains are estimated in carp inhabited in a certain pond. Furthermore, the three sub species of crucian carp have existed in the Tohoku area, and genetically divergent populations have been inhabited and brood in a pond. However, mtDNA variant patterns were common in both populations.
Cyprinidae家族中有许多鱼类,这些物种是从异种四倍体鱼类中演变而来的。然后,可以构建它们的复杂遗传特征和遗传结构。同学分析是分析种群结构,遗传差异和进化特征的非常有用的工具,但是它们可以分析长代。MINECHOCHRIALDNA(MTDNA)有望用作鱼类遗传学和渔业管理的敏感遗传标记。 However, in order to apply it for actual population study, a lot of issues should be resolved.The aims in the present study are (1) establishment an elementary method for purifying mtDNA in freshwater fish, (2) Clarifying a value of restriction fragment length polymorphism in carp by use a genetic marker of mtDNA, and (3) identification of species of crucian carp inhabited in Tohoku area in Japan.As the results, the可以根据化学方法(烷烃处理和非IDET P-40)建立纯化mtDNA的基本方法。接下来,基于该方法,在四种限制酶中观察到限制片段长度多态性,然后在居住在某个池塘中的鲤鱼中估计4种母体菌株。此外,在Tohoku地区存在三种克鲁克鲤鱼的三种种类,遗传上的种群被居住在池塘中。但是,mtDNA变体模式在两个人群中都是常见的。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
木島明博,谷口順彦,坂東喜久雄,向井龍男: "アルカリ処理法によるウナギ、コイ、アユのミトコンドリアDNA(mtDNA)の単離と塩基対数の測定" 水産育種. 15. 51-57 (1990)
Akihiro Kijima、Yoshihiko Taniguchi、Kikuo Bando、Tatsuo Mukai:“通过碱处理方法分离鳗鱼、鲤鱼和香鱼的线粒体 DNA (mtDNA) 和碱基对数的测量”渔业育种 15. 51-57 (1990)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kijima, A., N.Taniguchi, K.Bando, T.Mukai: "Rapid isolation of mitochondrial DNA by alkarine treatment and estimation of the number of their base pairs in Japanese eel, carp, and ayu" Fish Genet. Breed. Sci.15. 51-57 (1990)
Kijima, A.、N.Taniguchi、K.Bando、T.Mukai:“通过碱处理快速分离线粒体 DNA 并估计日本鳗鱼、鲤鱼和香鱼中碱基对的数量”《鱼类基因》。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
共 2 条
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Development of the damage copepod broken system for producyion of marine invertebrate seeds
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Research of genetic resources in the coastal intertidal zone around Oshika Peninsula
大鹿半岛沿岸潮间带遗传资源研究
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- 财政年份:1997
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GENETIC STUDY ON ALLOTETRAPLOIDY GOLDFISH AND CRUSSIAN CARP BY MATING EXPERIMENTS
异源四倍体金鱼和鲫鱼的交配遗传研究
- 批准号:0766023207660232
- 财政年份:1995
- 资助金额:$ 1.47万$ 1.47万
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Breeding structure in fish population by RFLP analysis of mitochondrial DNA
通过线粒体 DNA 的 RFLP 分析确定鱼类种群的繁殖结构
- 批准号:0266018602660186
- 财政年份:1990
- 资助金额:$ 1.47万$ 1.47万
- 项目类别:Grant-in-Aid for General Scientific Research (C)Grant-in-Aid for General Scientific Research (C)
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