Mapping and functional analysis of nucleus-cytoplasm compatibility gene in wheat

小麦核质相容性基因定位及功能分析

基本信息

批准号：
07660007
负责人：
NAKAMURA Chiharu
金额：
$ 1.47万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

An alien nucleus-cytoplasm compatibility gene from Triticum timopheevi (Ncc-tmp) was introduced into tetraploid wheat, Triticum durum, with the cytoplasm of Aegilops squarrosa. The following results were obtained using this nucleus-cytoplasm (NC) hybrid.1) Ncc-tmp controls gametophytic male fertility and zygotic viability of the NC hybrids under hemizygous condition.2) 4 RAPD markers that are closely linked with each other and with Ncc-tmp were selected, and using a series of D genome chromosome substitution lines Ncc-tmp was mapped on chromosome 1A.3) Sequences adjacent to Ncc-tmp was conserved uniquely in timopheevi group of wheat, and this sequences were also present in Triticum urartu, a A genome donor for tetraploid and hexaploid wheat.4) Segregation analysis of plump and shriveled seeds indicated the presence of two independent functional Ncc-tmp in T.timopheevi.5) NC hybrid-specific polymorphisms were detected in mitochondrial atp6-rps13 and nad3-orf156 by SSCP analysis and PCR-restriction enzyme analysis. These results set basis for fine mapping and cloning of Ncc genes that are the key genes controlling compatibility between nuclear genome and cytoplasmic genomes in the tribe Triticeae.6) In addition, NC hybrids of common wheat cv Chinese Spring with cytoplasms of 46 species from Triticum and Aegilops were studied to examine nuclear and cytoplasmic polymorphisms that are related to NC interactions. The study showed that in certain NC hybrids nuclear polymorphisms exist and are fully transmitted through all gametes. In one NC hybrid line, it was suggested that the cytoplasmic genome type was changed into the paternal genome type. These polymorphisms might be caused by NC interaction, thus leading to NC compatibility.

将来自Triticum timopheevi（NCC-TMP）的外来核核胞质兼容基因与Aegilops squarrosa的细胞质一起引入四倍体小麦Triticum Durum。使用该核细胞质（NC）杂交获得以下结果。1）NCC-TMP控制NC杂交的男配子性男性生育能力和在半合流状态下的二吻合率。选择-TMP，并使用一系列D基因组染色体取代线NCC-TMP在1A.3）上映射NCC-TMP在与NCC-TMP附近的序列上绘制在timopheevi小麦组中，并且在Timopheevi组中唯一保守，并且该序列也存在于Triticum urrarturartu urrartu urrartu urrartu ，一种用于四倍体和六倍体小麦的基因组供体。4）在T.Timopheevi.5）NC杂化特异性多态性中检测到浓度和萎缩种子的隔离分析表明，在T.timopheevi.5）中存在两个独立的功能性NCC-TMP。 NAD3-ORF156通过SSCP分析和PCR限制酶分析。这些结果设定了NCC基因精细映射和克隆的基础，NCC基因是控制核基因组和细胞质基因组之间兼容性的关键基因。并研究了烧烤，以检查与NC相互作用相关的核和细胞质多态性。该研究表明，在某些NC杂种中，存在所有配子，并通过所有配子完全传播核多态性。在一条NC杂种线中，有人提出细胞质基因组类型被改为父亲基因组类型。这些多态性可能是由NC相互作用引起的，因此导致NC兼容性。