Studies on the regulatory mechanisms of growth of cultured plant cells and organelles
培养植物细胞和细胞器生长调控机制的研究
基本信息
- 批准号:61560005
- 负责人:
- 金额:$ 1.54万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1986
- 资助国家:日本
- 起止时间:1986 至 1988
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Our research objectives were to understand the regulatory mechanisms of cell growth, differentiation and organellar development using cultured plant cells. We analyzed nucleic acid and protein syntheses, photosynthesis and respiratory activity during growth of tobacco cells at both the cellular and organellar levels. In addition, we analyzed a membrane-bound auxin-binding protein in tobacco which was thought to mediate auxin action in cell growth and differentiation.The results obtained are summarized as follows, i) photosynthetic activity developed in a 2,4-D-sustained photomixotrophic line of tobacco during growth in the light. The development of photosynthetic activity correlated with the synthesis of several chloroplast proteins including a small subunit of Rubisco and with the developwent of photosystem II activity; 2) heterotrophically grown tobacco cells showed growth cycle-related changes in the respiratory activity measured both at the cellular and mitochondrial levels. The ch … More anges in the respiratory activity occurred mainly through the cytochrome pathway, while the activity of the cyanide-resistant alternative pathway remained constant throughout the growth cycle. Kinds and amounts of sugars exogenously supplied did not affect the relative contribution of the alternative pathway. Our results show that the respiration is under strong control by adenylates and suggest that the alternative pathway plays a role other than an energy overflow; 3) a membrane-bound auxin-binding protein (MABP) was solubilized from cell suspension cultures of tobacco and characterized. The results show that MABP is a class of auxin-binding protein distinct from a cytosolic soluble type. Using an auxin-resistant variant and a transformant by agrobacterium tumefaciens, MABP was shown to be regulated by cytokinin and involved in auxin-mediated root differentiation. A role of MABP in root differentiation was also shown using an organ (root) culture system in which root differentiation could be manipulated. Less
我们的研究目的是细胞生长的法制机制,我们分析了在两个细胞ND细胞器水平的烟草细胞期间的核和蛋白质合成,光合作用和呼吸活性。细胞的生长和差异。结果总结如下,i)在2,4-d持续的光质营养线中发育的光合作用活性的速剂合成的发育与雪白色蛋白的合成相关的hototosynthetic的发展与rubisco的小亚基合成相关光系统的发育量II 2)异养生烟草细胞显示在细胞和线粒体水平上测量的呼吸活性的相关变化。 cons固定的生长周期不影响替代途径。结果表明,MABP是使用抗蛋白质的变体的胞质溶解性蛋白的蛋白质。可以操纵根部区分的系统
项目成果
期刊论文数量(13)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nagai,T.;et al.: Plant Cell Physiology. 30. 17-23 (1989)
Nagai,T.;等人:植物细胞生理学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Mennes, A. M. et al.: In D. Klambt, ed, Porc NASI, Poant hormone receptors, Springer Verlag, Berlin, Heidelberg, New York. (1988)
Mennes, A. M. 等人:D. Klambt 编辑,Porc NASI,Poant 激素受体,Springer Verlag,柏林,海德堡,纽约。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Nakamura, C.; Y. Hakui; T. Nagai; H. Ono: "The cyanide-resistant alternative pathway in heterotrophically grown Nicotiana tabacum cells in suspension culture" Plant Physiology.
中村,C.;
- DOI:
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- 影响因子:0
- 作者:
- 通讯作者:
Nagai, T.; C. Nakamura; T. Nagayoshi; H. Ono: "2,4-D-sustained photomixotrophic growth of a chlorophyllos cell suspension culture of Nicotiana tabacum" Plant Cell Physiology. 30. 17-23 (1989)
永井,T.;
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NAKAMURA Chiharu其他文献
NAKAMURA Chiharu的其他文献
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{{ truncateString('NAKAMURA Chiharu', 18)}}的其他基金
Molecular biological study and breeding of cold response in wheat
小麦冷反应的分子生物学研究及育种
- 批准号:
10044207 - 财政年份:1998
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Mapping and functional analysis of nucleus-cytoplasm compatibility gene in wheat
小麦核质相容性基因定位及功能分析
- 批准号:
07660007 - 财政年份:1995
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of cytoplasmic organellar function in wheat and its related species
小麦及其近缘种细胞质细胞器功能分析
- 批准号:
01560005 - 财政年份:1989
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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