A study on human thrombomodulin-immobilized antithrombogenic biomaterials

人血栓调节蛋白固定化抗血栓生物材料的研究

• 批准号: 07558258
• 负责人: AKASHI Mitsuru
• 金额: $ 1.15万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07558258/
• 关键词: human thrombomodulin; immobilization; antithrombogenicity; poly (ethylene) film; dialyzer; cellulose film; affinity interaction; surface plasmon resonance

Thrombomodulin (TM) is a newly described endothelial cell associated protein that functions as a potentnatural anticoagulant by converting thrombin from a protease to an anticoagulant. Recently, the preparation of reconbinant human thrombomodulin (hTM) was achieved by using gene technology, and since then biomaterials containing hTM have been of interest. In this project, we have done the hTM-immobilization onto various polymer surfaces such as poly (acrylic acid) or poly (vinylamine) grafted poly (ether urethane urea) or poly (ethylene) film using water soluble carbodiimide. As the results, it was apparent that hTM activity was retained enough to show its antithrombogenicity. And then hTM was immobilized onto small dialyzer consisting of cellulose film to carry out blood flow test. Moreover, hTM fragment (E456) was immobilized onto poly (ethylene) film and compared its amount of immobilized, activity and antithrombogenicity with native hTM.With regard to the amount of immobilized, it was apparent that the amount of immobilized of hTM fragment is higher than that of native hTM.Finally, as to compare the activity of native hTM and its fragment, the dissociation constants of f-hTM and thrombin by affinity interaction was evaluated using a surface plasmon resonance.

血小板调节蛋白（TM）是一种新描述的内皮细胞相关蛋白，通过将凝血酶从蛋白酶转化为抗凝剂，用作有效的抗凝剂。最近，通过使用基因技术来实现重新辅助人血栓形成蛋白（HTM）的制备，从那时起，含有HTM的生物材料就引起了人们的关注。在这个项目中，我们使用水溶性碳二酰亚胺进行了HTM-IMMBIBILIZATO在各种聚合物表面，例如聚（丙烯酸）或聚（乙烯基胺）接枝聚氨酯尿素）或聚（乙醚尿素尿素）或聚（乙烯尿素尿素）。结果，很明显，HTM活性足够保留以表明其抗稳定性。然后将HTM固定在由纤维素膜组成的小透析层上，以进行血液流程测试。 Moreover, hTM fragment (E456) was immobilized onto poly (ethylene) film and compared its amount of immobilized, activity and antithrombogenicity with native hTM.With regard to the amount of immobilized, it was apparent that the amount of immobilized of hTM fragment is higher than that of native hTM.Finally, as to compare the activity of native hTM and its fragment, the dissociation constants of使用表面等离子体共振评估F-HTM和通过亲和力相互作用的凝血酶。

项目成果

岸田晶夫 他: "ヒト・トロンボモジュリン-フラグメント(E456)の固定化と抗血栓性評価" 第46回高分子学会年次大会、横浜、1997年5月. 46(3). 345 (1997)

Akio Kishida 等人：“人血栓调节蛋白片段 (E456) 的固定化和抗血栓评价”第 46 届高分子科学学会年会，横滨，1997 年 5 月。46(3) (1997)。

岸田 晶夫 他: "トロンボモジュリンの医用材料への応用" 第7回バイオ・高分子シンポジウム、東京、1997年7月. 71-72 (1997)

Akio Kishida 等人：“血栓调节蛋白在医疗材料中的应用”第 7 届生物/聚合物研讨会，东京，1997 年 7 月。71-72 (1997)

岸田 晶夫 他: "ヒト・トロンボモジュリン-フラグメント(E456)の固定化と抗血栓性評価" 第46回高分子学会年次大会、横浜、1997年5月. 46(3). 345- (1997)

Akio Kishida 等人：“人血栓调节蛋白片段 (E456) 的固定化和抗血栓特性的评价”第 46 届高分子科学学会年会，横滨，1997 年 5 月。46(3)-(1997)。

A.Kishida, et al.: "Application of human thrombomodulin to medical materials." Proceeding of 7th Biopolymer Symposium. 7172 (1997)

A.Kishida 等人：“人血栓调节蛋白在医疗材料中的应用”。

岸田晶夫: "ヒト・トロンボモジュリン固定化材料の合成と抗血栓性評価" 人工臓器. (印刷中). (1996)

Akio Kishida：“人血栓调节蛋白固定材料的合成和抗血栓评价”人工器官（出版中）。