Development of hemostatic glue and devices for use in endoscopic surgery.

开发用于内窥镜手术的止血胶和装置。

基本信息

批准号：
07557358
负责人：
NAKAYAMA Yasuhide
金额：
$ 1.79万
依托单位：
National Cardiovascular Center Research Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

This research presents a novel photochemically-driven surgical tissue adhesive technology using photoreactive gelatins, which were partially derivatized with photoreactive groups, such as ultraviolet light (UV)-reactive benzophenone and visible light-reactive xanthene dye (e.g. fluorescein sodium salt, cosin Y,and rose bengal), anda water-soluble difunctional macromer [poly (ethylene glycol) diacrylate : PEGDA]. a series of photocurable tissue adhesive glues, which are viscous solutions when warmed, consisting of the photoreactive gelatin, PEDA, anda saline solution with or without ascorbic acidas a reducing agent, were prepared and evaluated for their effectiveness on hemostasis and anastomosis in cardiovascular surgery. Regardless of the type of photoreactive group examined, irradiation of the photocurable tissue adhesive glues by UV or visible light within 1 min, without excess heating, produced water-swollen gels, which had high adhesive strength to wet collagen film, due to the sy … More nergistic antion of photoreactive group-initiated photocrosslinking and photograft polymerization. As irradiation time increased, get yield increased and its ability to hold water decreased. a decrease in the molecular weight of PEGDA and an increase in concentration of both photoreactive gelatin and PEGDA resulted in a reduced ability to hold water and increased the tensile and burst strengths of the resultant gels. In rats in which the livers had been injured with a trephine during laparotomy, the bleeding spots were coated with the photocurable adhesive glue. Upon photoirradiation through an optical fiber, the coated solution was immediately converted to a swollen gel, which tightly adhered to the liver tissue, presumably by interpenetration and, concomitantly, hemostasis was completed. The anastomosis treatment with a photocurable adhesive glue in canine abdominal or thoracic aortas incised with a knife resulted in little bleeding under pulsatile flow after removing the clamps. Histological examination revealed that the adhesive glues photocured on rat liver surfaces gradually degraded in vivo with the infiltration of inflammatory cells and connectiv tissues, but without necrosis in the surrounding tissue. In addition, during laparoscopic surgery, percutaneous delivery of a photocurable adhesive glue and its in situ photogelation on rat liver surfaces was demonstrated using a specially designed fiberscope. These results suggest that the photocurable compound developed hear may serve as a biodegradable tissue adhesive glue for use in cardiovascular surgery and endoscopic surgery. Less

This research presents a novel photochemically-driven surgical tissue adhesive technology using photoreactive gelatins, which were partially derivatized with photoreactive groups, such as ultraviolet light (UV)-reactive benzophenone and visible light-reactive xanthene dye (e.g. fluorescein sodium salt, cosin Y, and rose bengal), and water-soluble difunctional MacRomer [聚（乙二醇）二丙烯酸酯：PEGDA]。一系列的光反应性组织粘附胶是在加热时是粘性溶液的，由有或没有抗坏血酸的光反应性明胶，PEDA和盐水溶液组成，并评估了还原剂，并评估了其对止血和心血管手术中吻合术的有效性。不管检查的光电反应性群体的类型如何，在1分钟内通过紫外线或可见光对光电组织粘合剂的辐射，没有过量加热，产生了水含水的凝胶，由于湿胶原薄膜具有很高的粘合力强度，这是由于感光化的组合量和照相胶片的较高的效果。随着辐照时间的增加，获得产量增加，其保持水的能力降低。 PEGDA的分子量降低以及光反应明胶和PEGDA的浓度增加，导致持有水的能力降低，增加了所得凝胶的拉伸和爆发强度。在剖腹手术过程中生命受伤的大鼠中，将出血斑覆盖在可光化粘合剂的情况下。通过光纤光辐射后，将涂层溶液立即转化为肿胀的凝胶，该凝胶紧密地粘附在肝组织上，大概是通过互穿渗透的，并且同时完成了止血。用刀片切开的腹部或胸动脉中的可光粘胶胶在切除夹具后在脉冲流下几乎没有出血。组织学检查表明，在大鼠肝脏表面上摄影的粘附胶在体内逐渐降解，随着炎症细胞和连接时机的浸润，但周围组织中没有坏死。此外，在腹腔镜手术期间，使用特殊设计的纤维镜证明了经皮递送可光的胶粘剂及其在大鼠肝脏表面上的原位光凝聚。这些结果表明，开发的可光化化合物可以用作可生物降解的组织粘合剂胶，用于心血管手术和内窥镜手术。较少的