Mechanisms of human sperm acrosome reaction

人类精子顶体反应机制

• 批准号: 07457378
• 负责人: ISHIKAWA Hiromichi
• 金额: $ 2.5万
• 依托单位: TOKYO DENTAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457378/
• 关键词: human sperm; acrosome; Ca; cAMP; Ca chanell; アセチルコリン; カフェイン

In the present study, we established the fluorescent label-high performance liquid chromatography (HPLC) system for simultaneous measurement of human sperm adenyl purines (ATP,ADP,AMP,adenosine, cAMP). This method suggested that all the adenyl purines including cAMP did not change in consequence of the acrosome reaction. Caffeine is most well known agent to increase human sperm acrosome reaction and its mode of action is considered to be appeared through the inhibition of phosphodiesterase (PDE) and subsequent accumulation of intracellular cAMP.The present study observed that human sperm motility, intracellular cAMP and the induction of acrosome reaction were significantly increased in the presence of caffeine, whereas the plasma membrane permeable cAMP derivatives, dibutylic-cAMP and 8Br-cAMP accelerated sperm motility but not the induction of acrosome reaction. We found acetylcholine (ACh) enhanced the acrosome reaction without increasing the motility and intracellular cAMP.Then this action was suppressed competitively by atropine, a muscarinic antagonist. The present results indicate that intracellular cAMP may play a role in sperm motility, whereas acceleration of the acrosome reaction by means of caffeine is not appeared through the inhibition of PDE.The acrosome reaction and motility were the events occurred in the acrosome and midpiece-tail, respectively.

本研究建立了荧光标记-高效液相色谱（HPLC）系统，用于同时测定人精子腺嘌呤（ATP、ADP、AMP、腺苷、cAMP）。该方法表明，包括 cAMP 在内的所有腺苷酸不会因顶体反应而发生变化。咖啡因是最著名的增加人类精子顶体反应的药物，其作用方式被认为是通过抑制磷酸二酯酶（PDE）和随后细胞内cAMP的积累而出现的。本研究观察到人类精子活力、细胞内cAMP和在咖啡因存在下，顶体反应的诱导显着增加，而质膜渗透性 cAMP 衍生物、二丁酸-cAMP 和 8Br-cAMP 加速精子运动，但不诱导顶体反应。顶体反应。我们发现乙酰胆碱（ACh）增强了顶体反应，但不增加运动性和细胞内cAMP。然后这种作用被毒蕈碱拮抗剂阿托品竞争性抑制。本研究结果表明，细胞内cAMP可能在精子运动中发挥作用，而通过抑制PDE，并没有出现咖啡因对顶体反应的加速作用。顶体反应和运动是发生在顶体和中尾的事件。 ， 分别。