Morphologic studies

形态学研究

• 批准号: 07457052
• 负责人: OSAMURA Yoshiyuki
• 金额: $ 3.14万
• 依托单位: Tokai University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457052/
• 关键词: Pituitary gland; Pit-1; Receptor; Immunohistochemistry; In situ hydridization; mRNA; In situ PCR; in situ PCR; 機能発現; 遺伝子発現; ホルモンレセプター; in situ hybridization; Pit-1遺伝子; pit-1遺伝子

In order to clarify the mechanisms of specific functional expression of the anterior pituitary cells, we have investigated the expressoins of Pit-1 isoforms. Pit-1 beta and Pit-1 T by immunohistochemistry (IHC) and in situ hydridization (ISH). In the human pituitary and pituitary adenomas, our results suggested the involvement of Pit-1 beta in the differentiation toward GH and Pit-1 T toward TSH,respectively.Recently, the synergetic roles of GH releasing hormone (GHRH) and receptor for estrogen (ER), for thyroid hormone (TR) and for retinoid (RAR,RXR) with Pit-1 have been reported and emphasized. By Combined ISH-IHC histochemical staining, ER and RXR were colocalized with Pit-1 in the same cells. In prolactin secreting adenomas, Pit-1 and ER were colocalized in the same cells. Regarding GHRH receptor (GHRHR), we are in the process of introducing in situ RT-PRCR in order to detect trivial amount of GHRHR mRNA.Preliminary, GHRHR has been co-localized in the same cells of the hyperplastic pituitary gland in hGHRH transgenic rats.Our studies have suggested the synergetic effects of Pit-1 isoforms and various receptors in the specific functional expressions of the anterior pituitary cell and in the pituitary adenoma cells. More detailed mechanisms remain to be investigated in the future investigations.

为了阐明垂体前叶细胞特异性功能表达的机制，我们对Pit-1亚型的表达进行了研究。通过免疫组织化学 (IHC) 和原位杂交 (ISH) 检测 Pit-1 beta 和 Pit-1 T。在人类垂体和垂体腺瘤中，我们的结果表明 Pit-1 beta 分别参与向 GH 和 Pit-1 T 向 TSH 的分化。最近，GH 释放激素（GHRH）和雌激素受体的协同作用（ ER）、甲状腺激素（TR）和类视黄醇（RAR，RXR）与 Pit-1 已被报道并强调。通过组合 ISH-IHC 组织化学染色，ER 和 RXR 与 Pit-1 在同一细胞中共定位。在催乳素分泌腺瘤中，Pit-1 和 ER 共定位于同一细胞中。关于GHRH受体（GHRHR），我们正在引入原位RT-PCRR以检测微量的GHRHR mRNA。初步发现，GHRHR已共定位于hGHRH转基因大鼠的增生性垂体的相同细胞中.我们的研究表明Pit-1亚型和各种受体在垂体前叶细胞和垂体的特定功能表达中具有协同作用腺瘤细胞。更详细的机制还有待未来的调查研究。

项目成果

Matsuno A., Osamura RY,et.al: "Changes in the ultrastructural distribution of prolactin and growth hormone mRNAs in pituitary cells of female rats after estrogen and bromocriptine treatment." Histochem Cell Biol. 104. 37-45 (1995)

Matsuno A.、Osamura RY 等人：“雌激素和溴隐亭治疗后雌性大鼠垂体细胞中催乳素和生长激素 mRNA 超微结构分布的变化。”

Sanno N., Teramoto A., Osamura R.Y: "Clinical and cytofunctional classification of pituitary adenomas." Acta Neurochirurgica (Wien). 138. 1186-1192 (1996)

Sanno N.、Teramoto A.、Osamura R.Y：“垂体腺瘤的临床和细胞功能分类。”

Sanno N.,Osamura RY.et al.: "Studies on GH and PRL gene expression by Non-radioisotopic in situ hybridization in TSH-secreting pituitary adenomas." J Clin Endocrinol Metab. 80. 2518-2522 (1995)

Sanno N.、Osamura RY.等人：“通过非放射性同位素原位杂交对分泌 TSH 的垂体腺瘤中 GH 和 PRL 基因表达进行的研究”。

Osamura RY., Sanno N., Teramoto A: "Clinical and cytofunctional classification of pituitary adenomas : Proposal of a new classification." Endocrine Pathology. 6. 253-256 (1995)

Osamura RY.、Sanno N.、Teramoto A：“垂体腺瘤的临床和细胞功能分类：新分类的提议。”

Sanno N., Osamura R.Y., et.al: "Combined Non-isotopic in situ hybridization method and indirect immunohistochemical analysis of hormone production in rat pituitary." J Clin Pathol (Mol Pathol). 49. M57-M60 (1996)

Sanno N.、Osamura R.Y. 等人：“结合非同位素原位杂交方法和间接免疫组织化学分析大鼠垂体激素的产生。”