Immunohistochemical localization of catecolamine synthesizing enzymes in human pheochromocytomas

人嗜铬细胞瘤儿茶酚胺合成酶的免疫组织化学定位

• 批准号: 63570149
• 负责人: OSAMURA Yoshiyuki
• 金额: $ 1.34万
• 依托单位: Tokai University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570149/
• 关键词: Pheochromocytoma; Adrenalin; Noradrenaline; Phenylethanolamine beta-methyltransferase; Dopamine beta-hydroxylase; Immunohistochemistry; Tyrosine beta hydroxylase; Tyrosineβ hydroxylase; 褐色細胞腫; カテコールアミン; エンケファリン; 免疫組織化学; カテコールアミン合成酵素; 副腎髄質

Abstract Total 17 (10 adrenal and 7 extra-adrenal) pheochromocytomas were examined immunohistochemically for the localization of catecholamine synthesizing enzymes, throsine hydroxylase (TH), dopamine 3-hydroxylase (DBH) and phenylethanolamine beta-methyltransferase (PNMT) as well as the marker peptides for pheochromocytomas, i.e. met-enkephalin-arg-gly-leu (MEAGL). The normal adult, fetal, newborn human adrenal medullae were also examined.6 adrenal pheochromocytomas showed immunohistochemical positive staining for PNMT, 5 of these cases demonstrated elevated serum adrenalin levels. This indicated morpho-functional correlation. PNMT positive cells were mostly positive for TH, but did not show colocalization of MEAGL except for one case. Absence of colocalization was considered to reflect physiological condition from the similar staining in the normal adult adrenal medulla. Primary culture and immunoelectron microscopy suggested the processing and synthesis of adrenalin and MEAGL in the secretory granules.7 extra-adrenal pheochromocytomas were negative for PNMT and suggested the lack of adrenalin synthesis.

摘要：对总共 17 例（10 例肾上腺和 7 例肾上腺外）嗜铬细胞瘤进行了免疫组织化学检查，以检测儿茶酚胺合成酶、苏氨酸羟化酶 (TH)、多巴胺 3-羟化酶 (DBH) 和苯乙醇胺 β-甲基转移酶 (PNMT) 的定位以及标记物用于嗜铬细胞瘤的肽，即甲硫氨酸脑啡肽-arg-甘氨酸-亮氨酸（MEAGL）。还检查了正常成人、胎儿、新生儿的肾上腺髓质。6例肾上腺嗜铬细胞瘤显示PNMT免疫组化阳性，其中5例血清肾上腺素水平升高。这表明形态功能相关性。 PNMT阳性细胞大多为TH阳性，但除1例外未显示MEAGL共定位。共定位的缺失被认为反映了正常成人肾上腺髓质中类似染色的生理状况。原代培养和免疫电镜显示分泌颗粒中存在肾上腺素和MEAGL的加工和合成。7个肾上腺外嗜铬细胞瘤PNMT阴性，提示肾上腺素合成缺乏。

项目成果

Osamura, Y., Suemizu, Y., Yasuda, N., Hiro, S., Onoda, N. and Joh, T.H.: "Immunohistochemical studies of catecholamine synthesis in human pheochromocytomas." Folia Endocrinol Japon 65:205 (Abstr), 1989.

Osamura, Y.、Suemizu, Y.、Yasuda, N.、Hiro, S.、Onoda, N. 和 Joh, T.H.：“人类嗜铬细胞瘤中儿茶酚胺合成的免疫组织化学研究。”

長村義之,末永洋志,安田納,堀貞明,小野田登,Tong.H.Joh: "ヒト褐色細胞腫におけるカテユ-ルアミン合成の免疫組織化学的検討" 日本内分泌学会誌. 65. 205 (1989)

Yoshiyuki Nagamura、Hiroshi Suenaga、Yasuda No、Sadaaki Hori、Noboru Onoda、Tong.H.Joh：“人类嗜铬细胞瘤中 Catylamine 合成的免疫组织化学研究”日本内分泌学会杂志 65. 205 (1989)。

Osamura,Yoshiyuki.,Kawai,K.,Hori,S.,Suemizu,H.,Onoda,N.,Tong H.Joh: "Immunohistochemical Localization of Catecholamine synthesizing enzymes in human pheochromocytomas" Endocrine Pathology.

Osamura，Yoshiyuki.，Kawai，K.，Hori，S.，Suemizu，H.，Onoda，N.，Tong H.Joh：“人嗜铬细胞瘤中儿茶酚胺合成酶的免疫组织化学定位”内分泌病理学。

長村義之、末水洋志、安田納、堀貞明、小野田登、Tong H.Joh: 日本内分泌学会誌. (1989)

Yoshiyuki Nagamura、Hiroshi Suemizu、No Yasuda、Sadaaki Hori、Noboru Onoda、Tong H.Joh：日本内分泌学会杂志（1989）。

Osamura, Y., Kawai, K., Hori, S., Suemizu, Y., Onoda, N. and Joh, T.H.: "Immunohistochemical localization of catecholamine synthesizing enzymes in human pheochromocytomas." Endocrine Pathology 1990.

Osamura, Y.、Kawai, K.、Hori, S.、Suemizu, Y.、Onoda, N. 和 Joh, T.H.：“人嗜铬细胞瘤中儿茶酚胺合成酶的免疫组织化学定位。”