Molecular and Cellular Biology of Tobacco BY-2 Cell Line

烟草 BY-2 细胞系的分子和细胞生物学

基本信息

批准号：
07304053
负责人：
NAGATA Toshiyuki
金额：
$ 7.3万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

This project has been intended and conducted to facilitate the acceleration of research progress and information change on the study of molecular and cellular biology of tobacco BY-2 cell line. This subject is quite unique, since this is only one available highly synchronized system in the world that has been developed by the principal investigator and has been distributed to more than 100 laboratories in at least 20 countries. This is indispensable in particular for the cell cycle studies and is being used in major laboratories in the world. Thus' it was intended in this study to facilitate the informational exchange and romote the progress of scientific studies using this cell line in this Country as well.As aconsequence after two years' efforts, it was shown by using highly synchronized cell populations that molecular mechanism of the organization of cortical microtubules has been clearly demonstrated and several types of kinesin molecules oscillated differently during cell cycle pr … More ogression. It is also revealed that a novel auxin-signal transduction chain is involved in the maintanance of cell division activity in tobacco BY-2cells. Furthermore, the development of a novel in vitro transcription system by one of collaborators using BY-2 cells facilitated further the popularity of this cell line at the international level. It was also shown that there are two different transport systems of proteins into vacuoles that are dependent on unique sequence in the C-terminal and N-terminal sequences of proteins, respectively. Conversion of proplastids to amyloplasts upon the treatment by cytokinin has also been analyzed by one investigator. Thus, it may be said that even within the short frame of two years significant progress of the understanding of the cellular and molecular biology on tobacco BY-2 has been attained, resulting in understanding the basic phenomenon of plant cells in general.In 1995, a public forum was held at the occasion of Annual Meeting of the Botanical Society of Japan to familiarize the topics of discussion in this group to much wider circles in this country. Responses from the audiences were quite significant, since some of these peoples would like to introduce this experimental system in their studies. Less

该项目的目的和实施是为了促进烟草 BY-2 细胞系分子和细胞生物学研究的进展和信息变化。该课题非常独特，因为这是唯一一个可用的高度同步系统。由主要研究者开发并已分发到至少 20 个国家的 100 多个实验室，这对于细胞周期研究来说是必不可少的，并且正在世界各地的主要实验室中使用。在这个经过两年的努力，通过高度同步的细胞群，揭示了皮质微管组织的分子机制。已被清楚地证明，几种类型的驱动蛋白分子在细胞周期过程中以不同的方式振荡。还揭示了一种新型的生长素信号转导链参与了烟草中细胞分裂活性的维持。此外，一位合作者使用 BY-2 细胞开发了一种新型体外转录系统，进一步促进了该细胞系在国际上的普及。还表明存在两种不同的蛋白质转运系统。细胞分裂素处理后原质体转化为淀粉体的过程分别依赖于蛋白质 C 端和 N 端序列的独特序列。因此，甚至可以说。在短短两年内，对烟草 BY-2 的细胞和分子生物学的理解取得了重大进展，从而总体上了解了植物细胞的基本现象。 1995 年，当时举行了一个公共论坛日本植物学会年会的目的是让该国更广泛的圈子熟悉该小组的讨论主题，观众的反应非常重要，因为其中一些人希望在他们的研究中引入这个实验系统。较少的