Elucidation of the regulation mechanism of gene expression in the totipotency of plant cells

植物细胞全能性基因表达调控机制的阐明

基本信息

项目摘要

Though the totipotency of plant cells is said to be the most characteristic features of plant and it is used as the main means of plant biotechnology, its molecular mechanism is least elucidated. In this study, the totipotency has been studied at the three phases ; the dedifferentiation of differentiated somatic cells, the maintenance of these cells that regain cell division activity and the redifferentiation of these cells to whole plants. Since two plant hormones of auxin and cytokinin have been shown to be most influential in all of these three processes, the molecular mode of these two plant hormones has been intensively studied in respective three stages. Regarding the dedifferentiation process, we have isolated three auxin-regulated genes, parA,parB and parC,from the early stage of cultured tobacco mesophyll protoplasts. Thus we tried to elucidate the transcriptional regulation of these genes. Our efforts identified auxin-responsive elements in these genes and in particular, in t … More he parB promoter. Furthermore, we have identified a protein that binds to one of auxin-responsive element, which is classified as one of b-ZIPs. We have also identified a cytokinin-regulated gene as the first case in this category. Though the characterization of this cytokinin-regulated gene is currently under study, it seems to belong to arabinogalactan-related proteins. Subsequently we have identified arcA gene as an auxin-regulated gene in the actively dividing tobacco BY-2 cells. arcA has been found to be a homologue of receptor for activated C-Kinase. Furthermore, a protein that could have protein-protein interaction with this arcA protein has been indentified by means of the yeast two-hybrid system. This protein is a homologue of beta-subunit of K^+-channel. Thus it became certain that there is a novel signal transduction chain down-stream of auxin application, resulting in the cell division of cultured cells.On the other hand, we have identified two auxin-regulated genes in the early stage of embryogenesis of carrot epidermis tissues. One is found to be a homologue of parA,while the other is found to be a homologue of low-molecular weight heat shock proteins.Thus in this study we have identified several molecules that are involved in different phases of totipotency. Though full details of totipotency still remains to be elucidated, general features of totipotency has been shown in this study. Less
尽管植物细胞的全能性被认为是植物最典型的特征,并被用作植物生物技术的主要手段,但其分子机制目前尚不清楚。本研究对全能性进行了三个阶段的研究。分化的体细胞的维持,恢复细胞分裂活性的细胞以及这些细胞重新分化为完整植物,因为生长素和细胞分裂素这两种植物激素已被证明对所有这三种激素影响最大。针对这两种植物激素的去分化过程,我们从早期培养的烟草叶肉原生质体中分离出了三个生长素调控基因parA、parB和parC。我们试图阐明这些基因的转录调控。我们的努力鉴定了这些基因中的生长素响应元件,特别是在 t … More he parB 启动子中。此外,我们还鉴定了一种蛋白质。与生长素响应元件之一结合,该元件被归类为 b-ZIP 之一,我们还发现了该类别中的第一个案例,尽管该细胞分裂素调节基因的特征目前正在研究中。 ,它似乎属于阿拉伯半乳聚糖相关蛋白,随后我们发现arcA基因是活跃分裂的烟草BY-2细胞中的生长素调节基因,并且发现arcA是受体的同源物。此外,通过酵母双杂交系统鉴定了一种可以与该 arcA 蛋白发生蛋白-蛋白相互作用的蛋白,该蛋白是 K^+ 通道的 β 亚基的同源物。由此可以确定,生长素的下游存在一条新的信号转导链,导致培养细胞的细胞分裂。另一方面,我们在胡萝卜表皮胚胎发生的早期阶段鉴定了两个生长素调控的基因。一种是parA的同源物,另一种是低分子量热休克蛋白的同源物。因此,在这项研究中,我们鉴定了几种参与全能性不同阶段的分子。全能性的细节仍有待阐明,本研究已显示出全能性的一般特征。

项目成果

期刊论文数量(27)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Sakai, T., Takahashi, Y.and Nagata, T.: "Analysis of the promoter of the auxin-inducible gene, parC,of tobacco." Plant Cell Physiol.37. 906-913 (1996)
Sakai, T.、Takahashi, Y. 和 Nagata, T.:“烟草生长素诱导基因 parC 启动子的分析”。
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Isida S.et al.: "The mode of expression and promoter analysis of the arcA gene,an auxin-regulated gene in tobacco BY-2 cells." Plant Cell Physiol.37. 439-448 (1996)
Isida S.et al.:“烟草 BY-2 细胞中生长素调节基因 arcA 基因的表达模式和启动子分析。”
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Kusaba,M.et al.: "A multiple-stimuli-responsive as-1-related element of parA gene confers responsiveness to cadmium but not copper." Plant Physiol.111. 1161-1167 (1996)
Kusaba,M.等人:“parA 基因的多重刺激响应 as-1 相关元件赋予对镉的响应性,但对铜没有响应性。”
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Ishida, S., Takahashi, Y.and Nagata, T.: "The mode of expression and promoter analysis of the arcA gene, an auxin-regulated gene in tobacco BY-2 cells." Plant Cell Physiol.37. 439-448 (1996)
Ishida, S.、Takahashi, Y. 和 Nagata, T.:“烟草 BY-2 细胞中生长素调节基因 arcA 基因的表达模式和启动子分析。”
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Mitsui, H., Hasezawa, S., Nagata, T.and Takahashi, H.: "Cell cycle-dependent accumulation of a kinesin-like protein, Kat B/C,in synchronized tobacco BY-2 cells." Plant Mol.Biol.30. 177-181 (1996)
Mitsui, H.、Hasezawa, S.、Nagata, T. 和 Takahashi, H.:“同步烟草 BY-2 细胞中驱动蛋白样蛋白 Kat B/C 的细胞周期依赖性积累。”
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NAGATA Toshiyuki其他文献

NAGATA Toshiyuki的其他文献

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{{ truncateString('NAGATA Toshiyuki', 18)}}的其他基金

Multilateral analysis of the regulatory mechanism of transcription factor BHLHE41/DEC2 expression and its clinical significance in lung cancer.
转录因子BHLHE41/DEC2在肺癌中表达调控机制及其临床意义的多方分析
  • 批准号:
    20K09181
  • 财政年份:
    2020
  • 资助金额:
    $ 22.27万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Elucidation of early stages of plant illustration in Japan
日本植物插画早期阶段的阐释
  • 批准号:
    15K12437
  • 财政年份:
    2015
  • 资助金额:
    $ 22.27万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Elucidation of the virus propagation-interference mechanism in plant cells and search for suppressing factors of this process
阐明病毒在植物细胞中繁殖的干扰机制并寻找该过程的抑制因素
  • 批准号:
    24657038
  • 财政年份:
    2012
  • 资助金额:
    $ 22.27万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Characterization of a novel plant cell division factor with high molecular mass
一种新型高分子量植物细胞分裂因子的表征
  • 批准号:
    19570032
  • 财政年份:
    2007
  • 资助金额:
    $ 22.27万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Elucidation of molecular mechanism of habituation of cultured plant cells
阐明培养植物细胞适应的分子机制
  • 批准号:
    13440237
  • 财政年份:
    2001
  • 资助金额:
    $ 22.27万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Elucidation of the new signal transition pathway regarding auxin
阐明生长素的新信号转换途径
  • 批准号:
    09440263
  • 财政年份:
    1997
  • 资助金额:
    $ 22.27万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular and Cellular Biology of Tobacco BY-2 Cell Line
烟草 BY-2 细胞系的分子和细胞生物学
  • 批准号:
    07304053
  • 财政年份:
    1995
  • 资助金额:
    $ 22.27万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Molecular mechanism of the elasticity of plant gene expression and signal transduction chains
植物基因表达和信号转导链弹性的分子机制
  • 批准号:
    04257101
  • 财政年份:
    1991
  • 资助金额:
    $ 22.27万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas

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