Assembling a system for measuring biological activity of micro-level particle in heterogenous system

组装异质系统中微米级颗粒生物活性测量系统

基本信息

批准号：
06554036
负责人：
YOSHIOKA Takahito
金额：
$ 3.97万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

New system for measuring the biological activity of micro-level particles, for example, planktonic and bacterial cells and their colony, in heterogeneous system was developed. This system, namaed LASMMA (Laser Semi-Microprobe Mass Analysis), is composed of YAG laser, infra-red microscope, quartz cell, gas purification line, and isotope ratio mass spectrometer (IRMS). This system was specialized for the ^<13>C-tracer experiment on phytoplanktonic photosynthesis.Optical system, by which laser beam was applied to the sample through object lens of IR microscope, was prepared. Using TV camera, micro-spot was identified during laser treatment. Cellulose filter, of which isotope ratio was known, was treated with laser for 30 to 60 minutes. After purification and manometric measurement, the CO_2 was diluted with standard CO_2 gas of which ^<13>C content was known. The gas was introduced into IRMS to determine the ^<13>C content. Although the value of cellulose by LASMMA was lower than that by standard method, it suggested that its precision was enough for tracer experiment.LSMMA method was applid in the field experiment. The ^<13>C uptake activity of Microcystis was etsimated in Lake Suwa. Colony of this alga was identified under microscope, and treated by laser. Its ^<13>C content increased from 1.09 atom% at the beginning of the experiment to 3.52 atom% after 24hr incubation. The ^<13>C content changed almost as same as that measured for particulate organic carbon (POC) by the usual way.It was confirmed that LASMMA method could be applicable for field tracer experiment. Moreover, it was suggested that LASMMA system might not be limited for tracer experiment, although the standard deviation measured was larger than usual way and the measurement took much time.For future application, LASMMA System manual was rounded off.

开发了用于测量异质系统中微观颗粒（例如浮游和细菌细胞及其菌落）生物活性的新系统。该系统名为LASMMA（激光半微探针质量分析），由YAG激光器、红外显微镜、石英池、气体净化线和同位素比质谱仪（IRMS）组成。该系统专门用于浮游植物光合作用的^ 13 C示踪实验。制备了激光束通过红外显微镜物镜照射到样品上的光学系统。使用电视摄像机，在激光治疗过程中识别出微点。用激光处理同位素比已知的纤维素过滤器30至60分钟。在纯化和测压测量之后，用标准CO_2气体稀释CO_2，其中的13 C含量是已知的。将气体引入IRMS以确定 13 C含量。尽管LASMMA测得的纤维素值低于标准方法，但表明其精度足以进行示踪实验。LSMMA方法应用于现场实验。估计了诹访湖中微囊藻的 13 C吸收活性。在显微镜下鉴定该藻类的菌落，并用激光处理。其^ 13 C含量从实验开始时的1.09原子％增加到24小时培育后的3.52原子％。 ^ 13 C含量的变化与常规方法测量颗粒有机碳(POC)的变化几乎相同。这证实了LASMMA方法可以适用于现场示踪实验。此外，有人建议LASMMA系统可能不限于示踪剂实验，尽管测量的标准偏差比通常的方法大并且测量花费很多时间。为了将来的应用，LASMMA系统手册被完善。