Assembling a system for measuring biological activity of micro-level particle in heterogenous system

组装异质系统中微米级颗粒生物活性测量系统

• 批准号: 06554036
• 负责人: YOSHIOKA Takahito
• 金额: $ 3.97万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06554036/
• 关键词: Heterogeneous system; Tracer experiment; YAG laser; Carbon stable Isotope; Mass spectrometer; 微小粒子; 赤外線顕微鏡; 赤外顕微鏡

New system for measuring the biological activity of micro-level particles, for example, planktonic and bacterial cells and their colony, in heterogeneous system was developed. This system, namaed LASMMA (Laser Semi-Microprobe Mass Analysis), is composed of YAG laser, infra-red microscope, quartz cell, gas purification line, and isotope ratio mass spectrometer (IRMS). This system was specialized for the ^<13>C-tracer experiment on phytoplanktonic photosynthesis.Optical system, by which laser beam was applied to the sample through object lens of IR microscope, was prepared. Using TV camera, micro-spot was identified during laser treatment. Cellulose filter, of which isotope ratio was known, was treated with laser for 30 to 60 minutes. After purification and manometric measurement, the CO_2 was diluted with standard CO_2 gas of which ^<13>C content was known. The gas was introduced into IRMS to determine the ^<13>C content. Although the value of cellulose by LASMMA was lower than that by standard method, it suggested that its precision was enough for tracer experiment.LSMMA method was applid in the field experiment. The ^<13>C uptake activity of Microcystis was etsimated in Lake Suwa. Colony of this alga was identified under microscope, and treated by laser. Its ^<13>C content increased from 1.09 atom% at the beginning of the experiment to 3.52 atom% after 24hr incubation. The ^<13>C content changed almost as same as that measured for particulate organic carbon (POC) by the usual way.It was confirmed that LASMMA method could be applicable for field tracer experiment. Moreover, it was suggested that LASMMA system might not be limited for tracer experiment, although the standard deviation measured was larger than usual way and the measurement took much time.For future application, LASMMA System manual was rounded off.

在异质系统中开发了用于测量微颗粒（例如浮游生物和细菌细胞及其菌落）生物学活性的新系统。该系统是NAMA的LASMMA（激光半探针质量分析），由YAG激光，红外显微镜，石英细胞，气体纯化线和同位素比率质谱仪（IRMS）组成。该系统专门用于浮游植物光合作用的 ^<13> c-跟踪实验。光学系统，通过该系统通过IR显微镜的对象镜头将激光束应用于样品上。使用电视摄像头，在激光治疗期间确定了微点。纤维素过滤器（其中的同位素比）已知，用激光处理30至60分钟。纯化和测量测量后，将CO_2用标准CO_2稀释，其 ^<13> C含量已知。将气体引入IRMS以确定 ^<13> C含量。尽管通过LASMMA的纤维素值低于标准方法的值，但它表明其精度足以用于示踪剂实验。LSMMA方法在现场实验中应用。在苏瓦湖中，将微囊藻的 ^<13> C摄取活性进行了观察。该藻类的菌落在显微镜下鉴定出来，并通过激光治疗。它的 ^<13> C含量从实验开始时的1.09原子％增加到24小时孵育后的3.52原子％。 ^<13> c含量的变化几乎与通过通常的方式测量的有机碳（POC）所测量的变化几乎相同。证实lasmma方法可以适用于现场示踪剂实验。此外，据建议，尽管测得的标准偏差比平常大，并且测量时间花费了很长时间。对于将来的应用，将LASMMA系统手册变成了圆形，但LASMMA系统可能不限于示踪剂实验。