Development fo a column material for chromatographic resolution, using an ion-exchanged adduct of clay-alubumin.

使用粘土-白蛋白的离子交换加合物开发用于色谱分离的柱材料。

• 批准号: 06554030
• 负责人: YAMAGISHI Akihiko
• 金额: $ 4.86万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06554030/
• 关键词: ALBUMIN; CLAY; OPTICAL RESOLUTION; HPLC

The present project has been initiated to develop a new column material for optical resolution, using an ion-exchange adduct of clay and albumin. A clay is a layr phyllosilicate capable of intercalating a positive ion in the interlayr spaces. Albumin is one of the globular proteins that have been utilized as a chiral modifier in a variety of chemical reactions. We aimed at combing the high ability of chiral recognition of albumin with the intercalating properties of a clay. In the first year of the project, we investigated the adsorption behaviors of albumin by colloidally dispersed sodium montmorillonite. As a result, it was revelealed that ablumin was adsorbed to an amount as high as 2g per 1g of a clay. In the second year, we attempted to see the reason for such high adsorption ability of a clay. A solid adduct of clay-albumin was investigated with solid state NMR and X-ray diffraction analyzes. In the next step, we prepared a column of the adduct for high performance liquid chromatography. As a result, we suceeded in resolving a useful chiral intermediate in organic synthese, benzoin, on the column. Finally we seaked for the optimum conditions for resolution, varying the solvent compositions, pH and temperature. The present results may open a way to develop a new colum for optical resolution on an industrial scale in a near future.

本项目已开始使用粘土和白蛋白的离子交换加合物来开发新的列材料，以用于光学分辨率。粘土是一种能够在层间空间中插入正离子的Layr phyllosilicate。白蛋白是在各种化学反应中被用作手性修饰剂的球状蛋白之一。我们的目的是将手性识别白蛋白的高能力与粘土的互化特性相结合。在项目的第一年，我们研究了胶体分散的蒙脱石钠的白蛋白的吸附行为。结果，人们认为阿布鲁明被吸附到每1克粘土的2g含量高达2g。在第二年，我们试图看到粘土如此高吸附能力的原因。用固态NMR和X射线衍射分析研究了粘土 - α-雄蛋白的固体加合物。在下一步中，我们为高性能液相色谱法准备了加合物的一列。结果，我们成功地解决了色谱柱上有机合成的有机手性中间体。最后，我们为分辨率的最佳条件而改变，改变了溶剂成分，pH和温度。目前的结果可能会在不久的将来以工业规模开发新的光学分辨率的新方法。

项目成果

兆・嶋津・山岸: "Mass Transport on a Clay-modified electrode as Studied by QCM Method" Chemistry Letters. 161-162 (1995)

Cho、Shimazu 和 Yamagishi：“通过 QCM 方法研究的粘土改性电极上的传质”化学快报 161-162 (1995)。

山岸・堀田: "粘土薄膜の電極修飾剤への応用" 粘土科学. 33. 233-245 (1994)

Yamagishi 和 Hotta：“粘土薄膜在电极改性剂中的应用”Clay Science 33. 233-245 (1994)。

Keiichi Inukai, Yuji Hotta and Akihiko Yamgishi: "Formation of a clay monolayr at an air-water interface." J.Chem. Soc., Chem. Comm.959. (1994)

Keiichi Inukai、Yuji Hotta 和 Akihiko Yamgishi：“在空气-水界面处形成单层粘土。”

佐藤・山岸: "Monte Carlo simulations on interaction of tris phenanthroline ruthenium (II) by clays" J. Phys. Chem.100. 1711-1715 (1995)

Sato 和 Yamagishi：“粘土与三菲咯啉钌 (II) 相互作用的蒙特卡罗模拟”J. Phys.100（1995）。

犬飼,堀田,谷口,渡村,山岸: "Formation of a Clay Monolage at a Air-Water Interface" Journal of Chemical Society,Chem.Comm.959-959 (1994)

Inukai、Hotta、Taniguchi、Watarimura、Yamagishi：“空气-水界面处粘土单体的形成”化学会杂志，Chem.Comm.959-959 (1994)