Study on the calcification by periodental ligament fibroblasts in the periodonta regeneration

牙周膜成纤维细胞钙化作用在牙周再生中的研究

基本信息

批准号：
06454540
负责人：
MURAYAMA Yoji
金额：
$ 3.78万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06454540/
关键词：
Periodontal ligament fibroblast Osteogenic function 1alpha, 25-dihydroxyvitamin D3 1alpha, 25-dihydroxyvitamin D3 receptor Fibroblast growth factor DNA synthesis chemotaxis 硬組織形成ビタミンD_3レセプター TGF-β EGF IGF 活性型ビタミンD_3 活性型ビタミンD_3レセ

项目摘要

In the aspect of periodontal regeneration, we focused on the regulation of the proliferation and the chemotaxis by fibroblast growth factor (FGF) and that of osteogenic functions by 1alpha, 25-dihydroxyvitamin D3 (vit.D3) in human periodontal ligament fibroblast (HPLF).Numbers of growth factors have been shown to induce cell migraiton as well as cell proliferation. To understand the mechanisms how the cells were driven to migration or proliferation upon same growth factor stimuli, we are interested in detecting the different phases of the cell of human fibroblasts undergoing migration. Cells migrated against FGF did not incorporate bromodeoxyuridine, suggesting that the cells in S phase were not induced to migrate. No dividing cells were found in the migrated cell population. However, migrated cells were found to express c-myc and c-fos mRNA,suggesting that these cells were exit from G0 phase of the cell cycle. Based upon the results, we conclude that the cells undergoing migration wer … More e most likely in G1 phase of the cell cycle.It has been shown that vit. D3 enhances alkaline phosphatase (ALP) activity and osteocalcin (OC) productivity in HPLF which have osteoblast-like cell functions. To evaluate the role of vit. D3 in ALP activity and OC productivity in HPLF,we examined the kinetics of gene expression of 1alpha, 25-dihydroxyvitamin D3 receptor (VDR), ALP activity and OC productivity at different phases of culture. The level of VDR gene expression, ALP activity and OC productivity were enhanced as culture cell density increased. Vit. D3 significantly enhanced ALP activity and OC productivity in each culture cell density. However, vit. D3 did not increase the level of VDR gene expression. The supernatant obtained from the culture of multilayred cells enhance the expression of the VDR genes in cells when the culture cell density was sparse. These sparse culture phase cells did not express the VDR gene and vit. D3 hardly affect ALP activity and OC productivity in these cells. Thus, if the sparse cells were previously stimulated with the culture supernatant, vit. D3 strongly enhanced OC productivity. These results suggest that vit. D3 enhances OC productivity and depends upon the level of VDR gene expression in HPLF culture cells and that the culture supernatant from multilayred cells contains the factors which increase VDR gene expression. Less

在牙周再生方面，我们专注于成纤维细胞生长因子（FGF）（FGF）的增殖和趋化性调节，以及1α-二羟基伏胺D3（VIT.D3）的成骨功能，人类周期性纤维纤维纤维细胞（HPLFF）的成长。增殖。为了了解在相同生长因子刺激下将细胞驱动到迁移或增殖的机制，我们有兴趣检测正在迁移迁移的人成纤维细胞细胞的不同阶段。针对FGF迁移的细胞未掺入溴脱氧尿苷，这表明S相中的细胞没有诱导迁移。在迁移的细胞种群中未发现分裂细胞。然而，发现迁移的细胞表达C-MYC和C-FOS mRNA，这表明这些细胞从细胞周期的G0阶段退出。基于结果，我们包括在细胞周期的G1阶段中最有可能进行迁移的细胞。它已显示为VIT。 D3在具有成骨细胞样细胞功能的HPLF中增强了醇磷酸酶（ALP）活性和骨钙素（OC）生产力。评估VIT的作用。 D3在HPLF中的ALP活性和OC生产力中，我们检查了1Alpha，25-二羟基维生素D3受体（VDR），ALP活性和OC生产力在培养的不同阶段的基因表达的动力学。随着培养细胞密度的增加，VDR基因表达，ALP活性和OC生产率的水平得到了提高。 VIT。 D3在每个培养细胞密度中显着提高了ALP活性和OC生产率。但是，vit。 D3没有增加VDR基因表达的水平。从多层细胞培养物获得的上清液会增强培养细胞密度稀疏时细胞中VDR基因的表达。这些稀疏的培养期细胞未表达VDR基因和VIT。 D3几乎不会影响这些细胞中ALP活性和OC生产力。如果先前用培养的上清液vit刺激了稀疏的细胞。 D3强烈提高了OC生产率。这些结果表明VIT。 D3提高了OC的生产率，并取决于HPLF培养细胞中VDR基因表达的水平，并且来自多层细胞的培养上清液包含增加VDR基因表达的因素。较少的