Pathological and Preventive Nutritional Studies on Environmental Factors of Cerebrovascular Dementia in the Japanese, from Neutritional Pathogenesis of Cerebrovascular Diseases.

从脑血管疾病的中性发病机制看日本人脑血管性痴呆环境因素的病理学和预防性营养研究。

• 批准号: 05304050
• 负责人: YAMORI Yukio
• 金额: $ 2.62万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05304050/
• 关键词: Stroke; Cerebrovascular Dementia; Immunohostochemistry; Perforating Arteries; Angionecrosis; Phagocytos; Actin; Monoclonal Antibody; モノクロープル抗体; Stroke; Cerebrovascular Dementia; Immunohostochemistry; Perforating Arteries; Angionecrosis; Phagocytos; Actin; Monoclonal Antibody; モノクロープル抗体

We studied the pathogenetic feature of cerebrovascular diseases by applying new immunohistochemical techniques according to the well-standardized study protocol among collaborating investigators : Brain specimens (1*1*0.5cm)including perforating and cortical arteries from autopsied cases of stroke and/or cerebrovascular dementia were obtained within 5 hours after death were fixed in methonal-Carnoy's fixative, embedded in paraffin and sectioned for routine pathalogical examination after Hematoxilin, Eosin, Masson, Azan-Mallory and Elastica van Gieson stains as well as for immunoperoxidase staining, using monoclonal antibodies against antiactin and antimacrophage antibodies.We demonstrated the degeneration and necrosis started at the outer layr of smooth muscle cells of perforating arteries with the infiltration of macrophages from Virchow-Robin's space and collagen proliferation, and at the advanced stage macrophages penetrated through endothelial cells and accumulated in the subendothelial space to induce occlusive lesions like vascular lesions in stroke-prone SHR.These findings support the importance of disturbance in the pathogenesis of cerebrovascular diseases in stroke and cerebrovascular dementia in the Japanese.This aspect was also supported by our comparative epidemiological survey on the nutrition of Japanese immigrants who had less incidence of stroke than the Japanese in Japan.

我们通过根据合作研究者的标准化研究方案应用新的免疫组织化学技术来研究脑血管疾病的致病特征：脑样本（1*1*0.5厘米）（1*1*0.5厘米），包括穿孔和皮质的动脉，包括中风和/或脑部固定的固定性，在5小时内均可获得固定的固定性，并在5个小时内获得了固定性，该病例是在5小时内获得的。催血素，曙红，马斯森，阿赞马利群和弹性抗体染色以及免疫氧化酶染色后，在石蜡中插入并进行了常规的疗法检查，并使用单克隆抗体抗体抗体和抗触发抗体抗体的平稳脉络均表现出平稳的脉络脉络。动脉随着巨噬细胞的渗透而从摩bro蛋白的空间和胶原蛋白增殖中，在高级阶段巨噬细胞中穿过内皮细胞的高级巨噬细胞，并积聚在内皮下空间中，以诱导闭塞性病变诱导诸如中风症状的刺激性的孔隙率刺激性的孔隙率，这些疾病的重要性是孔的重要性。日本人的痴呆症。我们的比较流行病学调查对日本移民的营养的比较流行病学调查也支持，而日本移民的营养比日本的日本人少。