Pathophysiological・Biochemical Cooperative Studies by Linkage Analysis on Hypertension Genes in SHR

SHR高血压基因连锁分析的病理生理生化协同研究

基本信息

批准号：
07308076
负责人：
YAMORI Yukio
金额：
$ 1.92万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07308076/
关键词：
Hypertension Stroke Gene Linkage analysis Renal function Cardiac hypertrophy Endocrine system Intracellular message transduction SHR SHRSP 食塩血管内皮 DNA 交雑実験

项目摘要

Linkage analyzes of genes related to hypertension and stroke were cooperatively carried out in the F_2 generation obtained by cross breeding of spontaneously hypertensive rats (SHR) or stroke-prone SHR (SHRSP) with their control normotensive Wistar-Kyoto (WKY) rats which had been established all as the reference strains such as SHR/Izm, SHRSP/Izm and WKY/Izm. These sets of F_2 rats were carefully observed for the development of hypertension, stroke and other phenotypes related to these pathophysiological conditions, and the following results of the linkage analyzes were obtained.1) Strong linkage with systolic blood pressure (SBP) was detected at 3 loci on Chromosome (Chr.) 1 (DIMIT2), Chr.3 (D3Mgh16) and Chr.4 (TRY1), and less strong linkage was observed at 2 loci on Chr.9 and 10. Blood pressure distribution in the F_2 rats was explained mostly by the genetic influence of these 5 loci.2) On Chr.1 the major gene related to SBP located within 35cM from gene loci of LSN and MYL2.3) Among the F_2 obtained from crosses between SHRSP/Izm and WKY/Izm. 9 rats developed stroke within 1 year after birth. One locus on Chr.4 was detected to associate with the development of stroke but not with blood pressure, indicating firstly the possible involvement of this region in the pathogenesis of stroke unrelated to hypertension itself.

与高血压和中风相关的基因的连锁分析是在通过自发性高血压大鼠（SHR）或可冲刺的SHR（SHRSP）及其对照正常性的Wistar-Kyoto（WKY）大鼠进行的F_2生成中进行的，这些大鼠已确定为参考菌株，例如SHR/IZM/IZM/IZM/izm iz/izm izm和izm izm和iz。仔细观察了这些与这些病理生理条件相关的高血压，中风和其他表型的发展，并获得了连锁分析的以下结果。1）与收缩压（SBP）的强链连接以下结果（chr.1（dimit2），chr.3mgh1（drmgh1）（dy3mgh1），在Chr.9和10的2个基因座中观察到F_2大鼠的血压分布主要是通过这5个基因座的遗传影响来解释的。2）对Chr.1 Chr.1与SBP相关的主要基因在LSN和MYL2.3的Gene logi logi colts of shrsp/izm/izm和wky/izm之间获得。 9只大鼠在出生后1年内发展中风。检测到Chr.4上的一个基因座与中风的发展相关，但与血压相关，首先表明该区域可能参与与高血压本身无关的中风发病机理。