Visceral afferent mechanisms in the gastrointestinal tract

胃肠道内脏传入机制

• 批准号: 05044188
• 负责人: TAMURA Kenji
• 金额: $ 5.12万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05044188/
• 关键词: Enteric Nervous System,; Substance P,; Afferent nerve; Sensory Neuron,; Seconel messenger,; G protein,; Calbindin; 直腸; 胃; モルモット; 迷走神経; 骨盤神経

In the present project we intended to test the hypothesis that G-proteins coupled both excitatory receptors of myenteric AH neurons. Intracellular methods of electrical recording were used to investigate the effects of GTP-gamma-s and pertussis toxin (PTX) on sloW EPSPs. as well as excitatory responses to substance P (SP). lmpalements were maintained for 5.5hours to evaluate the effects of PTX.Slow EPSPs and SP responses declined progressively and disappeared after 3.75hrs. Hyperpolarizing after-potentials were unaffected by PTX.GTP-gamma-s injection resulted in a progressive increase in slow EPsP-like behavior. Atter GTP-gamma-s, SP-ioduced depolarization was infinitely prolonged resulting in inability to evoke responses after an initial response. The results are consistent with the involvement of G-protein in coupling excitatory receptors in AH! type 2 neurans. The second purpose of this project was to test the hypothesis that the expression of calbindin immunoreactivity(lR)in distal colonic myenteric neurons can be correlated with their electrophysiological and morphological characteristics. After analysis of calbindin-lR,the precise morphology of NB-labeled neurons was revealed by immunoperoxidase histochemistry. All of the MLP neutons and 13% of SLP neurons were calbindin-lR.AH/Type 2 physiological properties were recorded from 3 SLP neurons not expressing calbindin-lR.Six of the SLP neurons were calbindin-lR,4 had S/type 1 and 2 had neither S/Type l nor AH/type 2 electrical properties. These results suggest that the distribution of calbindin-lR in distal colonic myenteric neurons is different from that in the small intestine in the guinea pig. We conclude that calbindin-lR can be used as an indicator or predictor of neither the morphology nor the electrophysiology of myenteric neurons in the guinea pig distal colon.

在本项目中，我们旨在检验以下假设：G蛋白与肌室AH神经元的两个兴奋性受体耦合。电内记录的细胞内方法用于研究GTP-GAMMA-S和百日咳毒素（PTX）对慢EPSP的影响。以及对P（SP）物质的兴奋反应。维持5.5小时的LMPALEMENT，以评估PTX.Slow EPSP的影响，SP响应逐渐下降，并在3.75小时后消失。超极化后电位不受PTX的影响。GTP-GAMMA-S注射导致慢速EPSP样行为逐渐增加。 ATTER GTP-GAMMA-S，SP碘化的去极化无限期延长，导致初始响应后无法引起反应。结果与G蛋白参与AH偶联兴奋性受体的参与是一致的！ 2型神经元。该项目的第二个目的是检验以下假设：远端结肠肌植物神经元中钙蛋白免疫反应性（LR）的表达与它们的电生理学和形态学特征有关。在分析了Calbindin-LR之后，免疫过氧化物酶组织化学揭示了NB标记神经元的精确形态。所有MLP中性子和13％的SLP神经元均为calbindin-lr.Ah/2型生理特性，记录了3个不表达calbindin-lr的SLP神经元的SLP神经元的calbindin-lr，4个calbindin-lr，4个s/type 1和2型和2型和2型L型L或类型L型l也没有AH/类型2型电气。这些结果表明，calbindin-LR在远端结肠肌植物神经元中的分布与豚鼠中小肠的分布不同。我们得出的结论是，Calbindin-LR可以用作豚鼠远端结肠中肌植物神经元的形态和电生理学的指标或预测指标。

项目成果

Kenji Tamura and Paul Wade: "Electrophysiology of morphologicallyidentified myenteric neurons in guinea pig distal colon." American Journal of Physiology.

Kenji Tamura 和 Paul Wade：“豚鼠远端结肠形态学鉴定的肌间神经元的电生理学。”