Mechanism of nervous interaction between the enteric nervous system and the autonomic nervous system

肠神经系统与自主神经系统的神经相互作用机制

• 批准号: 02044134
• 负责人: TAMURA Kenji
• 金额: $ 5.95万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02044134/
• 关键词: enteric nervous system; myenteric plexus; autonomic nervous system; morphology vagus nerve; pelvic nerve; C fiber; 胃; FastーEPSPs; アウェルバッハ壁内神経; ルシファ-イエロ-; 胃壁神経; 下行結腸; Yペプチド; 直腸; C線維

This project has been designed to investigate the synaptic relationships between the myenteric plexus in the guinea pig stomach, small intestine, distal colon and rectum and the extrinsic autonomic innervations to these parts of the gastrointestinal tract. In order to address this final target, two experimental steps have been made. As a first step, stomach or distal colonic myenteric neurons were stained intracellularly with Lucifer yellow or neurobiotin to Characterize the cellular morphology. Two morphological cell types were identified in the distal colon. One was neurons with a single long process (SLP) and multiple short processes in various forms and the other was neuron which had multiple long process (MLP) emanating a oval smooth surfaced cell body with or without short processes. Electrical properties of MLP neurons were always those of the AH/type II neurons but SLP neurons showed various characteristics those of including AH/type 2, S/typo 1, Single spike and Type 3 neurons. In the stomach myenteric plexus, no MLP neuron was found and all of the neurons showed short spike durations. In the next step of the project, intracellular recordings were made from stomach and distal colonic myenteric neurons while vagus and pelvic nerves were stimulated extracellularly. Synaptic potentials evoked by extrinsic nerve stimulations were only fast excitatory postsynatpic potentials (fast-EPSPs) with long time delays. No slow synaptic input was found either stomach or distal colonic myenteric plexus. In the colonic myenteric plexus fast-EPSPs were found only in the SLP neurons which had shorter spike duration than MLP neurons. It appeared that extrinsic excitatory inputs from central nervous system to the myenteric plexus were medicated by C fibers in the form of fast-EPSPs and only to the SLP neurons.

该项目旨在研究豚鼠胃中的骨髓神经丛之间的突触关系，小肠，远端结肠和直肠以及与胃肠道这些部分的外部自主神经神经支配。为了解决这个最终目标，已经进行了两个实验步骤。第一步，胃或远端结肠肌植物神经元在细胞内用露西夫黄色或神经益素染色，以表征细胞形态。在远端结肠中鉴定出两种形态学细胞类型。一个是具有单个长过程（SLP）和多种形式的多个短过程的神经元，另一个是神经元的神经元，其神经元具有多个长过程（MLP），发出有或没有短过程的椭圆形光滑浮出水面的细胞体。 MLP神经元的电性能始终是AH/II型神经元的电性能，但SLP神经元显示出各种特征，包括AH/型2，S/Typo 1，单峰和3型神经元。在胃肌丛中，未发现MLP神经元，所有神经元显示出短的峰值持续时间。在该项目的下一步中，细胞内记录是由胃和远端结肠肌元神经元进行的，而迷走神经和骨盆神经被细胞外刺激。外部神经刺激引起的突触电位仅是长时间延迟的快速兴奋性伴有后脉冲电位（快速EPSP）。未发现胃突触的缓慢输入或远端结肠肌丛。在结肠myenteric plexus中，快速EPSP仅在SLP神经元中发现的峰值持续时间比MLP神经元短。似乎从中枢神经系统到肌丛的外在兴奋性输入被C纤维以快速EPSP的形式和仅针对SLP神经元的形式进行了药物治疗。

项目成果

Kenji Tamura: "Pelvic inputs to myenteric neurons in the guinea pig distal colon" American Journal of Physiology. (1992)

Kenji Tamura：“骨盆输入到豚鼠远端结肠肌间神经元”美国生理学杂志。

Kenji Tamura: "Morphological and electrophysiological properties of the myenteric plexus neurons in the guinea pig distal colon" American Journal of Physiology. (1992)

Kenji Tamura：“豚鼠远端结肠肌间丛神经元的形态和电生理特性”美国生理学杂志。

A.Kuwahara & K.Tamura: "Extrinsic control of submucosal plexus neurons in the guinea pig small intestine." American Journal of Physiology.

桑原

Kenji Tamura and Michael Schemann: "Morphological characteristics and the vagal inputs of the guinea pig gastric myenteric plexus" American Journal of Physiology. (1992)

Kenji Tamura 和 Michael Schemann：“豚鼠胃肌间神经丛的形态特征和迷走神经输入”美国生理学杂志。

Kenji Tamura,Hidesuke Takahira and Kyuichiro Onoe: "Anal sphincters:Their electrical activities and mechanism of neural regulation" CRC press, 22 (1992)

Kenji Tamura、Hidesuke Takahira 和 Kyuichiro Onoe：“肛门括约肌：它们的电活动和神经调节机制”CRC Press，22 (1992)