Structural elucidation of isolated and fractionated lignins by means of ultra-high resolving mass spectrometry (LignoMassStructure)

通过超高分辨率质谱分析分离和分馏木质素的结构 (LignoMassStructure)

• 批准号: 525499342
• 负责人: Professor Dr. Steffen Fischer
• 金额: --
• 依托单位: Institut für Analytische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/525499342?language=en
• 关键词: Structural; elucidation; isolated; fractionated; lignins

The goal of this research project is a development of ESI- and GALDI-FT-ICR-MS and tandem MS routines for a characterization of isolated and fractionated lignins from different plants (coniferous wood, deciduous wood and annual plants) and pulping processes (kraft, sulfite and soda pulping). These routines will massively help to reduce the effort for an analysis of soluble and insoluble lignin samples, in comparison to classical analytical methods for a lignin characterization (e.g., GPC, NMR, Py-GC/MS). On the one hand, general sample information are accessible with these FT-ICR-MS and -MS/MS methods, such as molar mass distribution. On the other hand, also specific structural insights into the lignin samples are obtainable by these experiments. For this purpose, method development and optimization experiments will be performed with the help of a fractionated kraft lignin from deciduous wood (beech). In addition, a hetero spectroscopic correlation approach will be applied to combine the FT-ICR-MS and -MS/MS results with results from other analytical techniques (Py-GC/MS, 13C-, 31P- and 2D-HSQC-NMR spectroscopy), which will help to obtain more information about the structural composition of the different lignins. These novel results will significantly increase our understanding of the influence of an isolation process on the lignin target structure. Especially the GALDI-FT-ICR-MS and -MS/MS analyses of solid lignin samples will be helpful to characterize the lignin structure more detailed, independently from the solubility of the lignin compounds contained in the different samples or sample fractions. In the last project phase, the capabilities of the developed methods will be demonstrated by analyzing a complex lignocellulose, which will help to evaluate the applicability of the developed routines for other biopolymers (cellulose and hemicellulose). If this method application step is successful, for the first time ever, a structural characterization of biopolymers in their native environment is possible. Therefore, the results from this research project will have an impact on the development of more effective and expedient isolation and modification procedures for biopolymers, which will significantly contribute to a production of lignin and lignocellulose based products of higher value in the near future.

该研究项目的目的是开发Esi-ft-ft-ICR-MS以及串联MS例程，以表征来自不同植物（针叶木，落叶木材和年度植物）以及制浆过程（牛皮纸，硫酸盐，亚硫酸盐和苏打水）的分离和分馏的木质素。与木质素表征的经典分析方法相比，这些例程将大大帮助减少对可溶性和不溶性木质素样品进行分析的努力（例如GPC，NMR，PY-GC/MS）。一方面，这些FT-ICR-MS和-MS/MS方法可以访问一般的样本信息，例如摩尔质量分布。另一方面，这些实验也可以获得对木质素样品的特定结构见解。为此，将借助落叶木（Beech）的分离牛皮纸木质素（Beech）进行方法开发和优化实验。此外，将采用杂体相关方法将FT-ICR-MS和-MS/MS的结果与其他分析技术（PY-GC/MS，13C/MS，13C，31P-HSQC-NMR光谱）相结合，这将有助于获得有关不同Lignignins的结构组成的更多信息。这些新颖的结果将大大增加我们对隔离过程对木质素靶点结构的影响的理解。尤其是对固体木质素样品的GALDI-FT-ICR-MS和-MS/MS/MS/MS/MS分析将有助于对木质素结构进行更详细的表征，而与不同样品或样品分数中包含的木质素化合物的溶解度无关。在最后一个项目阶段，将通过分析复杂的木质纤维素来证明开发方法的功能，这将有助于评估开发的常规对其他生物聚合物（纤维素和半纤维素）的适用性。如果此方法应用步骤是有史以来首次成功的，那么在本机环境中生物聚合物的结构表征是可能的。因此，该研究项目的结果将影响生物聚合物的更有效和方便的隔离和修改程序的开发，这将在不久的将来有助于产生木质素和木质纤维素基于较高价值的产品。