High-resolution Ultra Performance Liquid Chromatography (UPLC) for Quantification of Biopolymers in Complex Matrices and Micropollutants in Water and Wastewater

高分辨率超高效液相色谱 (UPLC) 用于定量复杂基质中的生物聚合物以及水和废水中的微污染物

基本信息

  • 批准号:
    RTI-2023-00468
  • 负责人:
  • 金额:
    $ 9.18万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Research Tools and Instruments
  • 财政年份:
    2022
  • 资助国家:
    加拿大
  • 起止时间:
    2022-01-01 至 2023-12-31
  • 项目状态:
    已结题

项目摘要

We propose to purchase a new ultra performance liquid chromatography (UPLC) with photodiode array detector (PDA) to support research programs at the University of Northern British Columbia (UNBC). We need fast, high precision detection of both small molecules and high-molecular weight biopolymers with high sensitivity in complex sample matrices. The UPLC is a salient infrastructure requirement for at least five researchers at UNBC. The research programs to be supported by the acquisition of this equipment include: resource recovery from biological wastewater treatment system and biosolids, removal of micropollutants in municipal wastewaters, natural product discovery from Northern Boreal insect microbiomes, transport of nutrients, trace elements and organic contaminants (such as antibiotics) in the vadose zone, and impacts of diseases on tree health and forest resources. This system is equipped with a flow-through needle autosampler, a column heater, and a PDA UV-Vis detector. This equipment has the capability of handling up to four-solvent mobile phases, a computer, monitor, peripherals, and operational software. The software can analyze qualitatively and quantitatively both small and large molecules. The proposed system would allow easy method development and management. It is critical that our research team secures the UPLC system to accurately identify and quantify both small molecules and polymers in complex samples. With the resolution and analysis speed obtained from this state-of-the-art system, our team will process troublesome matrices and samples with many different analytes. Compared to standard high-performance liquid chromatography systems, this UPLC system would provide advanced analysis, flexibility to study a wide range of samples, shorter run times and increased sensitivity and selectivity of analytes allowing for a higher sample throughput. The new UPLC system will also be able to handle the desired range of concentrations for individual components in our complex samples. Highly qualified personnel (HQP) recruited in our research programs will receive training on how to use the UPLC system. After training, the HQP will frequently use the UPLC for research on resource (biopolymers and amino acids) recovery from aerobic granular sludge systems treating wastewater, removal of micropollutants in municipal wastewater, natural product discovery, quantification of antibiotics in leachates and biosolids, and complex chemical mixtures in natural matrices such as foliage and bark. Thus, the requested UPLC will be in almost continual use by the applicant, co-applicants, collaborator, and other users. 9 PhD, 16 Masters, and 28 undergraduate students will be trained on the equipment in the next five years.
我们建议购买带有光电二极管阵列检测器 (PDA) 的新型超高效液相色谱 (UPLC),以支持北不列颠哥伦比亚大学 (UNBC) 的研究项目。我们需要以高灵敏度快速、高精度地检测复杂样品基质中的小分子和高分子量生物聚合物。 UPLC 是 UNBC 至少五名研究人员的一项重要基础设施要求。收购该设备将支持的研究项目包括:从生物废水处理系统和生物固体中回收资源,去除城市废水中的微污染物,从北寒带昆虫微生物组中发现天然产物,营养物质、微量元素和有机污染物的运输(渗流区的抗生素等),以及疾病对树木健康和森林资源的影响。该系统配备了流通式针头自动进样器、柱加热器和 PDA 紫外-可见检测器。该设备能够处理多达四种溶剂流动相、计算机、显示器、外围设备和操作软件。该软件可以定性和定量分析小分子和大分子。所提出的系统将允许轻松的方法开发和管理。我们的研究团队确保 UPLC 系统能够准确识别和量化复杂样品中的小分子和聚合物至关重要。凭借从这一最先进的系统获得的分辨率和分析速度,我们的团队将处理具有许多不同分析物的棘手基质和样品。与标准高性能液相色谱系统相比,该 UPLC 系统将提供先进的分析、研究各种样品的灵活性、更短的运行时间以及更高的分析物灵敏度和选择性,从而实现更高的样品通量。新的 UPLC 系统还将能够处理复杂样品中各个成分所需的浓度范围。我们的研究项目中招募的高素质人员 (HQP) 将接受有关如何使用 UPLC 系统的培训。培训结束后,总部将经常使用 UPLC 进行以下方面的研究:从处理废水的好氧颗粒污泥系统中回收资源(生物聚合物和氨基酸)、去除城市废水中的微污染物、天然产物发现、渗滤液和生物固体中抗生素的定量以及复合物天然基质(如树叶和树皮)中的化学混合物。因此,所请求的 UPLC 将被申请人、共同申请人、合作者和其他用户几乎持续使用。未来五年将有9名博士生、16名硕士生和28名本科生接受该设备的培训。

项目成果

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Iorhemen, Oliver其他文献

Iorhemen, Oliver的其他文献

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{{ truncateString('Iorhemen, Oliver', 18)}}的其他基金

Resource recovery from biological wastewater treatment system and biosolids
生物废水处理系统和生物固体的资源回收
  • 批准号:
    RGPIN-2022-05350
  • 财政年份:
    2022
  • 资助金额:
    $ 9.18万
  • 项目类别:
    Discovery Grants Program - Individual
Resource recovery from biological wastewater treatment system and biosolids
生物废水处理系统和生物固体的资源回收
  • 批准号:
    DGECR-2022-00527
  • 财政年份:
    2022
  • 资助金额:
    $ 9.18万
  • 项目类别:
    Discovery Launch Supplement
Resource recovery from biological wastewater treatment system and biosolids
生物废水处理系统和生物固体的资源回收
  • 批准号:
    RGPIN-2022-05350
  • 财政年份:
    2022
  • 资助金额:
    $ 9.18万
  • 项目类别:
    Discovery Grants Program - Individual
Resource recovery from biological wastewater treatment system and biosolids
生物废水处理系统和生物固体的资源回收
  • 批准号:
    DGECR-2022-00527
  • 财政年份:
    2022
  • 资助金额:
    $ 9.18万
  • 项目类别:
    Discovery Launch Supplement
Stability of aerobic granular sludge: role of extracellular polymeric substances (EPS)
好氧颗粒污泥的稳定性:胞外聚合物(EPS)的作用
  • 批准号:
    533044-2019
  • 财政年份:
    2021
  • 资助金额:
    $ 9.18万
  • 项目类别:
    Postdoctoral Fellowships
Stability of aerobic granular sludge: role of extracellular polymeric substances (EPS)
好氧颗粒污泥的稳定性:胞外聚合物(EPS)的作用
  • 批准号:
    533044-2019
  • 财政年份:
    2021
  • 资助金额:
    $ 9.18万
  • 项目类别:
    Postdoctoral Fellowships
Stability of aerobic granular sludge: role of extracellular polymeric substances (EPS)
好氧颗粒污泥的稳定性:胞外聚合物(EPS)的作用
  • 批准号:
    533044-2019
  • 财政年份:
    2020
  • 资助金额:
    $ 9.18万
  • 项目类别:
    Postdoctoral Fellowships
Stability of aerobic granular sludge: role of extracellular polymeric substances (EPS)
好氧颗粒污泥的稳定性:胞外聚合物(EPS)的作用
  • 批准号:
    533044-2019
  • 财政年份:
    2020
  • 资助金额:
    $ 9.18万
  • 项目类别:
    Postdoctoral Fellowships
Stability of aerobic granular sludge: role of extracellular polymeric substances (EPS)
好氧颗粒污泥的稳定性:胞外聚合物(EPS)的作用
  • 批准号:
    533044-2019
  • 财政年份:
    2019
  • 资助金额:
    $ 9.18万
  • 项目类别:
    Postdoctoral Fellowships
Stability of aerobic granular sludge: role of extracellular polymeric substances (EPS)
好氧颗粒污泥的稳定性:胞外聚合物(EPS)的作用
  • 批准号:
    533044-2019
  • 财政年份:
    2019
  • 资助金额:
    $ 9.18万
  • 项目类别:
    Postdoctoral Fellowships

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超高性能液相色谱高分辨率质谱仪 (UHPLC-HRMS)
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