Use of receptor-type oncogene c-ergB-2 for the diagnosis and treatment of human cancers

受体型癌基因c-ergB-2在人类癌症诊断和治疗中的应用

• 批准号: 04557019
• 负责人: MORI Shigeo
• 金额: $ 10.37万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04557019/
• 关键词: oncogene; breast cancer; serodiagnosis; steroid hormone; c-erbB-2; ELISA; RIA; 受容体; 膀胱癌; 癌胎児性蛋白

a. We established the method for quantitation of serum c-ErbB-2 protein levels with the introduction of ELISA and RIA.The outcome was shown to be stable and reliable. This system is now under evaluation for clinical use at the Ministry of Welfare.b. The following new findings were obtained concerning the c-erbB-2 gene, product, and related genes.i The Estradiol was shown to work as a ligant for the c-ErbB-2 protein.ii New receptor-type tyrosine kinase brt was found.iii A new oncogenic chimeric protein tyrosine kinase P80 was identified.iv The promoter site of c-erbB-2 gene was revealed.v Fgr, a src-type tyrosine kinase was shown to associate with IgG Fc receptor II.

一个。我们通过引入ELISA和RIA来确定定量血清C-ERBB-2蛋白水平的方法。结果证明结果稳定且可靠。现在，该系统正在评估福利部的临床使用。获得了以下新发现，以涉及c-erbb-2基因，产物和相关基因。i雌二醇被证明可以作为C-erbb-2蛋白的韧带起作用。 V fgr显示，SRC型酪氨酸激酶与IgG FC受体II相关。

项目成果

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Inoue K：“WTI 作为一种新的预后因素和检测急性白血病微小残留病的新标志物。”

Jiro Fujimoto: "brt,a mouse gene encoding a novel receptor-type protein-tyrosine kinase,is preferentia Ug expressed in the brain." Oncogene. 9. 693-698 (1994)

Jiro Fujimoto：“brt，一种编码新型受体型蛋白酪氨酸激酶的小鼠基因，优先在大脑中表达Ug。”

Shiota M.: "Hyperphosphorylation of a novel 80-kDa protein-tyrosine kinase in a human Ki-1 lymphomacell line,AMS3." Oncogene. 9. 1567-1574 (1994)

Shiota M.：“人类 Ki-1 淋巴瘤细胞系 AMS3 中新型 80 kDa 蛋白酪氨酸激酶的过度磷酸化。”

Sugano K: "Demonstration of ErbB-2 protein excreted from milk by RIA." Gan-no-rinsho. 40. 921-930 (1994)

Sugano K：“通过 RIA 演示从牛奶中排出 ErbB-2 蛋白。”

Shiota M,Fujimoto J,Takenaga M,Satoh H,Ichinohasama R,Abe M,Yamamoto T,MOri S: "Diagnosis of t (2 ; 5) (p23 ; q35) -associated Ki-1 lymphoma with immunohistochemistory" Blood. 84. 3648-3652 (1994)

Shiota M、Fujimoto J、Takenaga M、Satoh H、Ichinohasama R、Abe M、Yamamoto T、MOri S：“免疫组织化学诊断 t (2 ; 5) (p23 ; q35) 相关 Ki-1 淋巴瘤”血液。