Pilot Study of Adrenal hormones

肾上腺激素的初步研究

• 批准号: 6887987
• 负责人: JOANNE F DORGAN
• 金额: $ 8.49万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-23 至 2006-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6887987
• 关键词: androgens; breast neoplasms; cancer risk; clinical research; cortisol; dehydroepiandrosterone; diagnosis quality /standard; female; hormone related neoplasm /cancer; human subject; postmenopause; pregnenolone; progesterone; serology /serodiagnosis; women' s health

DESCRIPTION (provided by applicant): Breast Cancer is a hormonally dependent cancer, and it has recently been shown that postmenopausal women with elevated serum levels of androgens as well as estrogens are at an increased risk of developing breast cancer. Of particular interest, postmenopausal women with high levels of the adrenal androgens, dehydroepiandrosterone (DHEA) and its sulfate (DHEAS), are approximately twice as likely to develop breast cancer compared to those with lower levels. In the proposed pilot study, we will obtain preliminary data requested by reviewers for a revised R01 application to prospectively determine if increased enzymatic activity at specific step(s) in the steroidogenesis pathway is responsible for increased adrenal androgens in postmenopausal women who develop breast cancer. Specific aims for the proposed pilot study are: 1) quantify the reproducibility of laboratory assays to measure serum pregnenolone, 17OH-pregnenolone, progesterone, 17OH-progesterone, cortisol, and 11-deoxycortisol; and 2) determine if a single measurement of pregnenolone, 17OH-pregnenolone, progesterone, 17OH-progesterone, cortisol, and 11-deoxycortisol adequately reflects longer-term levels. For aim 1, serum aliquots from the same blood draw from 4 women who donate blood to the Fox Chase Biosample Repository will be measured multiple times in multiple assay batches. Coefficients of variation and intraclass correlation coefficients will be calculated to evaluate assay reproducibility. For aim 2, hormones will be measured in serum collected at two different times from 20 women who donated serum to the Columbia, MO Serum Bank. Intraclass correlation coefficients will be calculated to evaluate whether hormone measurements in a single aliquot reflect longer term levels.

描述（由申请人提供）： 乳腺癌是一种荷尔蒙依赖性的癌症，最近已显示，雄激素水平升高的绝经后妇女以及雌激素的乳腺癌风险增加。特别有趣的是，与具有较低水平的肾上腺雄激素（DHEA）及其硫酸盐（DHEA）的绝经后女性，肾上腺抑制剂（DHEA）及其硫酸盐（DHEA）的可能性大约是患乳腺癌的可能性的两倍。在拟议的试点研究中，我们将获得审阅者要求对R01进行修订的申请要求的初步数据，以预期确定在类固醇生成途径中特定步骤的酶活性是否增加，导致多肾上腺雄激素在绝经后妇女中增加了肾脏癌。拟议的试点研究的具体目的是：1）量化实验室测定方法的可重复性，以测量血清妊娠，17OH-pregnenolone，孕激素，17OH-预生殖器，皮质醇和11-脱氧皮质醇； 2）确定对妊娠，17OH-释怀烯醇，孕酮，17OH-预糖剂，皮质醇和11-脱氧皮质醇的单一测量是否足以反映长期的水平。对于AIM 1，从4名妇女抽血的血清等分试样将血液捐赠给Fox Chase Biosample存储库的血清等分试样将在多次测定批次中进行多次测量。将计算变异系数和类内相关系数，以评估测定可重复性。对于AIM 2，将以20次不同时间收集的血清中测量激素，这些血清与20名捐赠血清的哥伦比亚血清库的女性。将计算出类内相关系数，以评估单个等分试样中的激素测量是否反映了长期水平。