Molecular Phylogeny of the Wild Populations of Medaka, Oryzias latipes

青鳉野生种群的分子系统发育

基本信息

批准号：
03640637
负责人：
SAKAIZUMI Mitsuru
金额：
$ 1.41万
依托单位：
The Tokyo Metropolitan Institute of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03640637/
关键词：
Oryzias latipes medaka mitochondrial DNA RFLP sequence data 12srDNA cytochrome b ND4 D-1oop 制限酵素

项目摘要

Oryzias latipes includes four genetically distinct groups, the Northern population, the Southern population, the East Korean population and the china-West Korean population on the basis of electrophoretic data.The Northern population and the Southern population were distinguished by RFLP (using eight 6-base enzymes) of mitochondrial DNA. The former included 10 haplotypes that were composed of a few groups closely related to each other. The latter included 89 haplotypes that could be divided into several groups with a lower level of difference. Sequence divergence was estimated to be 10.1% between a "northern" haplotype (haplotype 1) and a "southern" haplotype (haplotype 22).This value was thought to be very high as those between intraspecific haplotypes.We made two libraries of wkole mitochondrial DNA from a "northern" haplotype (1) and a "southern" haplotype (32). Three different regions encoding 12srRNA, cyt. b and ND4 were sequenced. The sequence differences between "northern" and " southern" haplotypes were 3.9% (15/586) in 12srDNA, 11.6% (64/554) in cyt. b and 9.9% (22/223) in ND4 region. Although the values differed among different regions, sequence divergence values were close to the estimate (10.1%) from RFLP analyses.These results indicated that the Northern population and the Southern population are highly differentiated from a view point of molecular phylogeny.

根据电泳数据，Oryzias latipes包括四个遗传上不同的群体：北方群体、南方群体、东朝鲜群体和中西朝鲜群体。北方群体和南方群体通过RFLP（使用8个6-线粒体 DNA 的碱基酶）。前者包括10个单倍型，由几个彼此密切相关的群体组成。后者包括 89 个单倍型，可以分为几个差异程度较低的组。 “北方”单倍型（单倍型 1）和“南方”单倍型（单倍型 22）之间的序列分歧估计为 10.1%。这个值被认为与种内单倍型之间的值相比非常高。我们制作了两个 wkole 线粒体文库来自“北方”单倍型 (1) 和“南方”单倍型 (32) 的 DNA。编码 12srRNA、细胞色素的三个不同区域。对b和ND4进行测序。 “北方”和“南方”单倍型之间的序列差异在12srDNA中为3.9%(15/586)，在细胞色素中为11.6%(64/554)。 b 和 ND4 区域中的 9.9% (22/223)。尽管不同地区的值有所不同，但序列分歧值接近RFLP分析的估计值（10.1%）。这些结果表明，从分子系统发育的角度来看，北方群体和南方群体存在高度分化。