ENU mutagenesis and a trial to isolate mutant alleles for specific genes located in the sex-determining region of the Japanese medaka.

ENU 诱变和分离位于日本青鳉性别决定区域的特定基因突变等位基因的试验。

• 批准号: 08680788
• 负责人: SAKAIZUMI Mitsuru
• 金额: $ 1.66万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680788/
• 关键词: Medaka; sex chromosome; sex-determining gene; mutagenesis; ethylnitrosourea; sex reversal; 動原体マッピング; 組換抑制領域

Ethylnitrosourea mutagenesis of spermatogonia in male medaka was performed to isolate mutant alleles in the sex-determining region. We used a congenic strain, Hd-rR.Y^<HNI>, the male of which has a genetic background derived from the Hd-rR strain, and has only the chromosomal region including the sex-determining factor (and r locus) from the HNI strain in its Y chromosome.1. Sex-reversed XY females were mated with XY males, and YY males were obtained. A sex-linked DNA marker SL1 was used for detection of YY males.2. YY (RR ; red) and XY (Rr ; red) males were mutagenized with 1mM ENU and mated with non-treated XX (rr ; white) females.3. A total of 746 offspring (402 males and 344 females) were produced from XY males. All females were white and 391 males red. Eleven white males were demonstrated to be sex-reversed XX individuals by mating expriment. All 269 male off spring from the YY males were red.4. Thus no mutants were obtained in the presentstudy. In order to get mutant alleles in the sex-determining region, it is necessary to continue mutagenesis experiment.

进行了雄性Medaka中精子症的乙基硝酸诱变，以分离性别确定区域的突变等位基因。我们使用了先天性菌株HD-RR.Y^<hni>，其雄性具有源自HD-RR菌株的遗传背景，并且仅具有包括性别确定因子（和R基因座）的染色体区域其Y染色体中的HNI菌株1。与XY雄性交配的性转向XY女性，并获得了YY雄性。与性别连接的DNA标记SL1用于检测YY雄性2。 YY（RR; RED）和XY（RR; RED）雄性用1MM ENU诱变，并与未处理的XX（RR; White）女性交配3。 XY雄性总共产生了746个后代（402名男性和344名女性）。所有女性均为白色和391名男性红色。通过交配征收，有11名白人男性被证明是XX个体。 YY雄性的所有269名男性均为红色。4。因此，在当前的研究中没有获得突变体。为了在性别确定的区域获得突变等位基因，有必要继续诱变实验。

项目成果

Matsuda, M., Yamagishi, T., Sakaizumi, M.Jeon S-R.: "Mitochondrial DNA variation in the Korean wild population of medaka, Oryzias latipes." Korean J.Limnol.30. 119-128 (1997)

Matsuda, M.、Yamagishi, T.、Sakaizumi, M.Jeon S-R.：“韩国野生青鳉种群的线粒体 DNA 变异。”

Matsuda, M., Yonekawa, H., Hamaguchi S.Sakaizumi, M.: "Geographic variation and diversity in the mitochondrial DNA of the medaka, Oryzias latipes, as determined by restriction endonuclease analyasis." Zool.Sci.14. 517-526 (1997)

Matsuda, M.、Yonekawa, H.、Hamaguchi S.Sakaizumi, M.：“通过限制性内切核酸酶分析确定青鳉 Oryzias latipes 线粒体 DNA 的地理变异和多样性。”

Matsuda, M., Kusama, T., Kurihara, Y., Oshiro, T., Hamaguchi, S.Sakaizumi, M.: "Isolation of a sex chromosome-specific DNA sequence in the medaka, Oryzias latipes." Genes Genet.Syst.72. 263-268 (1997)

Matsuda, M.、Kusama, T.、Kurihara, Y.、Oshiro, T.、Hamaguchi, S.Sakaizumi, M.：“青鳉 Oryzias latipes 中性染色体特异性 DNA 序列的分离。”

Matsuda M., Matsuda C., Hamaguchi S., Sakaizumi M.: "Identification of the sex chromosomes of the medaka, Oryzias latipes, by fluorescence in situ hybridization." Cytogenet.Cell Genet.82. 257-263 (1998)

Matsuda M.、Matsuda C.、Hamaguchi S.、Sakaizumi M.：“通过荧光原位杂交鉴定青鳉 Oryzias latipes 的性染色体。”

Matsuda, Masaru: "Isolation of a sex chromosome-specific DNA sequence in the medaka, Oryzias latipes." Genes Genet.Syst.,. 72 :. 263-268 (1997)

Matsuda, Masaru：“在青鳉 Oryzias latipes 中分离出性染色体特异性 DNA 序列。”