Molecular Genetics of Sex chromosomes of the medaka Oryzias latipes

青鳉性染色体的分子遗传学

基本信息

批准号：
06640796
负责人：
SAKAIZUMI Mitsuru
金额：
$ 0.64万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06640796/
关键词：
Medaka sex chromosome PCR recombination rate sex difference sex reversal 性転換性決定領域組替率

项目摘要

Using a male-determining factor-congenic strain of the Japanese medaka, Oryzias latipes, we screened a genomic DNA library and isolated two sex-linked DNA clones, SL-5 and SL-110. The nucleotide sequences were completely determined. SL-5 was a single-copy sequnece 1.7 kb in size. SL-110 was a repetitive sequence 498 b in length.We designed PCR primer sets at both ends of these clones. Length of the PCR product using the SL-5 primer sets was different between two inbred strains, Hd-rR and HNI.The PCR product using SL-110 primer sets gave different cleavage patterns between these strains when degested by Hae III.Segregation of a total of four loci, which included two DNA loci (SL-5 and SL-110), a pigment-pattern locus (r) and a male-determining factor (y), were examined using backcrosses of Hd-rR and HNI.No recombinant progeny were obtained from F1 males of the reciprocal crosses. By contrast, many recombinant progeny (>25%) were obtained from F1 females between SL-5 and SL-110.These results indicates that marked difference in recombination rate exists between XY nales and XX females. Such recombination restriction in XY males may have allowed the establishment of the orange-red male and white female strain, d-rR.In order to elucidate the mechanism of recombination restriction, segregation analyzes of sex-reversed fish are in progress.

我们使用日本Medaka的男性决定性因子 - 综合菌株，Oryzias latipes，我们筛选了一个基因组DNA库，并分离了两个性别连接的DNA克隆SL-5和SL-110。完全确定核苷酸序列。 SL-5是单拷贝Sequnece的大小1.7 kb。 SL-110是一个重复的序列498 b。我们在这些克隆的两端设计了PCR底漆集。使用SL-5引物组的PCR产物的长度在两个近交菌株HD-RR和HNI之间不同。使用SL-1110引物组的PCR产物在HAE III中脱发时，这些菌株之间的裂解模式不同。在HAE III中脱离了四个基因座的总数，其中包括四个基因座，其中包括两型DNA（SL-5和SL-5和SL-5和SL-5），一个sl-5和一个cortn and-sl-5），一个cortn and a cortn and a cort and sl-5），一个corpate and a cort and-perter and-perter-a corter and a cort and-sl-cment-pater and a cort and-sl-cment-n corti。（y）使用HD-RR和HNI的反向进行检查，并从相互交叉的F1雄性获得了重组后代。相比之下，从SL-5和SL-1110之间的F1女性获得了许多重组后代（> 25％）。这些结果表明，XY Nales和XX女性之间存在重组率的明显差异。 XY雄性的这种重组限制可能允许建立橙红色的雄性和白人雌性菌株，D-rr.为了阐明重组限制的机制，对性转化鱼的隔离分析正在进行中。