The etiological relation of Epstein-Barr virus to human uveitis

EB病毒与人类葡萄膜炎的病因关系

基本信息

批准号：
03670838
负责人：
USUI Masahiko
金额：
$ 1.54万
依托单位：
Tokyo Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670838/
关键词：
Epstein-Barr virus Polymerase chain reaction Uveitis Vogt-Koyanagi-Harada disease Receptor Retinal Pigment epithelium Chronic active EBV infection 慢性活動性EBV感染症 Epstein-Barr Virus ウェスタンブロッテング法 HLA-DR抗原 nucleocapsid glycoprotein Epste

项目摘要

In order to investigate the etiological relationship of Epstein-Barr virus (EBV) to human uveitis, measurement of EBV antibody levels and detection of EBV DNA by the polymerase chain reaction (PCR) method was performed on various samples from patients with uveitis of unknown etiology. Our results showed that first, among cases of acute anterior uveitis, conventionally regarded to be strongly associated with HLA-B27, EBV antibody levels (VCA IgM) were elevated. However, these cases were all negative for HLA B27, and thus may represent a newly recognized type of uveitis. Second, EBV antibody levels and peripheral EBV-infected lymphocytes were found to be elevated in certain cases of RPE disease, suggesting a relationship with EBV. Third, the presence of EBV DNA and EBV antibody was found in the cerebrospinal fluid of patients with Harada's disease. A high amino acid correlation is reported in the literature between the third hypervariable region of the HLA-DR antigen, that is highly associated with Harada's disease, and gp110, the nucleocapsid glycoprotein present in the nucleus and cytoplasm of cells infected with reproducing EBV. We found that lymphocytes from patients with acute Harada's disease display proliferation responses to peptides incorporating this common amino acid sequence, thus lending support to a specific association between EBV and patients with Harada's disease. Lastly, we looked for expression of the EBV receptor in intraocular tissues from normal autopsy eyes with immunohistological stains and western blotting using OKB7,a monoclonal antibody that recognizes part of the EBV receptor. EBV receptor expression was found to be present in the RPE and parts of the ciliary body, therefore indicating the possibility of direct intraocular infection by EBV.

为了探讨EB病毒（EBV）与人类葡萄膜炎的病因关系，对不明原因葡萄膜炎患者的多种样本进行了EBV抗体水平的测定和EBV DNA的聚合酶链反应（PCR）检测。。我们的结果表明，首先，在传统上被认为与 HLA-B27 密切相关的急性前葡萄膜炎病例中，EBV 抗体水平 (VCA IgM) 升高。然而，这些病例的 HLA B27 均为阴性，因此可能代表一种新认识的葡萄膜炎类型。其次，在某些 RPE 疾病病例中，发现 EBV 抗体水平和外周 EBV 感染淋巴细胞升高，表明与 EBV 相关。第三，在原田氏病患者的脑脊液中发现了EBV DNA和EBV抗体的存在。文献报道，HLA-DR 抗原的第三个高变区（与原田氏病高度相关）和 gp110（存在于感染 EBV 复制细胞的细胞核和细胞质中的核衣壳糖蛋白）之间存在高度氨基酸相关性。我们发现，急性原田氏病患者的淋巴细胞对包含这种常见氨基酸序列的肽表现出增殖反应，从而支持 EBV 与原田氏病患者之间的特定关联。最后，我们通过免疫组织学染色和使用 OKB7（一种识别部分 EBV 受体的单克隆抗体）进行蛋白质印迹，寻找正常尸检眼眼内组织中 EBV 受体的表达。发现 EBV 受体表达存在于 RPE 和睫状体部分，因此表明 EBV 直接眼内感染的可能性。