The etiological relation of Epstein-Barr virus to human uveitis

EB病毒与人类葡萄膜炎的病因关系

基本信息

批准号：
03670838
负责人：
USUI Masahiko
金额：
$ 1.54万
依托单位：
Tokyo Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670838/
关键词：
Epstein-Barr virus Polymerase chain reaction Uveitis Vogt-Koyanagi-Harada disease Receptor Retinal Pigment epithelium Chronic active EBV infection 慢性活動性EBV感染症 Epstein-Barr Virus ウェスタンブロッテング法 HLA-DR抗原 nucleocapsid glycoprotein Epste

项目摘要

In order to investigate the etiological relationship of Epstein-Barr virus (EBV) to human uveitis, measurement of EBV antibody levels and detection of EBV DNA by the polymerase chain reaction (PCR) method was performed on various samples from patients with uveitis of unknown etiology. Our results showed that first, among cases of acute anterior uveitis, conventionally regarded to be strongly associated with HLA-B27, EBV antibody levels (VCA IgM) were elevated. However, these cases were all negative for HLA B27, and thus may represent a newly recognized type of uveitis. Second, EBV antibody levels and peripheral EBV-infected lymphocytes were found to be elevated in certain cases of RPE disease, suggesting a relationship with EBV. Third, the presence of EBV DNA and EBV antibody was found in the cerebrospinal fluid of patients with Harada's disease. A high amino acid correlation is reported in the literature between the third hypervariable region of the HLA-DR antigen, that is highly associated with Harada's disease, and gp110, the nucleocapsid glycoprotein present in the nucleus and cytoplasm of cells infected with reproducing EBV. We found that lymphocytes from patients with acute Harada's disease display proliferation responses to peptides incorporating this common amino acid sequence, thus lending support to a specific association between EBV and patients with Harada's disease. Lastly, we looked for expression of the EBV receptor in intraocular tissues from normal autopsy eyes with immunohistological stains and western blotting using OKB7,a monoclonal antibody that recognizes part of the EBV receptor. EBV receptor expression was found to be present in the RPE and parts of the ciliary body, therefore indicating the possibility of direct intraocular infection by EBV.

为了研究爱泼斯坦 - 巴尔病毒（EBV）与人葡萄膜炎的病因关系，测量EBV抗体水平以及通过聚合酶链反应（PCR）方法检测EBV DNA方法（PCR）方法是对来自未知病理学葡萄膜炎患者的各种样品进行的。我们的结果表明，首先，在急性前葡萄膜炎的病例中，通常认为与HLA-B27密切相关，EBV抗体水平（VCA IGM）升高。但是，这些病例对HLA B27都是阴性的，因此可能代表了新认识的葡萄膜炎类型。其次，在某些RPE疾病的情况下发现EBV抗体水平和周围EBV感染的淋巴细胞升高，表明与EBV有关系。第三，在原始疾病患者的脑脊液中发现了EBV DNA和EBV抗体的存在。在HLA-DR抗原的第三个高变量区域之间的文献中报道了高氨基酸的相关性，该区域与原始疾病高度相关，而GP110（核）存在于细胞核和细胞中的细胞质中的Nucleocapsid糖蛋白。我们发现，来自急性原田疾病患者的淋巴细胞表现出对结合这种常见氨基酸序列的肽的增生反应，因此借用了EBV与Harada病患者之间特定关联的贷款支持。最后，我们从正常的尸检眼睛中寻找EBV受体在具有免疫组织学染色和使用OKB7的蛋白质印迹的眼内组织中的表达，这是一种识别部分EBV受体的单克隆抗体。发现EBV受体表达存在于睫状体的RPE和部分中，因此表明EBV直接眼内感染的可能性。