The elucidation for the mechanism of induction on intractable uveoretinitis with heat-shock protein

热休克蛋白诱导顽固性葡萄膜视网膜炎机制的阐明

基本信息

批准号：
09671817
负责人：
USUI Masahiko
金额：
$ 1.02万
依托单位：
Tokyo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Heat-shock protein 60 derived from Yersinia enterocolitica (Yersinia HSP60) and bovine retinal HSP (Retina HSP60) were previously identified by immunological cross reation and a high degree of common antigenicity, and a specific antibody against both was detected in the sera of intractable uveitis patients. We isolated and purified Retina HSP60 and Yersinia HSP60. Uveitis in rats following inoculation with these proteins was observed. Cross-reaction for both Retina and Yersinia HSP60 is suggested. It has been also suggested that the 65kDa of HSP of Streptococcus in recurrent aphthae within the oral cavity may be involved in the uveoretinitis of Behcet's disease, possibly through sensitization of the immune system. We examined serum antibody titers for various members of the 60 kDa family of HSPs and their implications relating to a role for HSP60s especialy focused in the Behcet's disease. We isolated the HSP60 of Streptococcus from the margin of oral aphthae in patientand the HSP60s o … More f Yersinia, retinoblastoma, and bovine retinat, and investigated the reaction of each of these HSP60s with 100-fold diluted serum samples from 20 Behcet's disease patients, with by ELISA. The anti-Streptococcus HSP60 antibody and anti-retinal HSP60 antibody titers of the 100-fold diluted serum were both significantly higher than those of similarly diluted serum samples from healthy donors. The results of ELISA antibody titer assay showed that, although the various HSP60s share a common basic antigenicity, they differed in reactivity to the anti-HSP60 antibodies in the sera of the Behcet's disease patients. The results indicate that subtle but significant differences exist in the antigenicity of the various HSP60s tested, all of which share a common basic antigenicity and are of approximately the same molecular weight, and suggest that an immuno cross-reaction between retinal and Streptococcal HSPs and a related autoimmune response may be involved in the development of intarctable uveoretinitis, especialy, Bechet's disease. Less

先前通过免疫交叉反应鉴定了源自小肠结肠炎耶尔森氏菌（Yersinia HSP60）和牛视网膜HSP（Retina HSP60）的热休克蛋白60，其具有高度的共同抗原性，并且在顽固性葡萄膜炎的血清中检测到针对两者的特异性抗体我们在接种这些药物后在大鼠中分离并纯化了视网膜 HSP60 和葡萄膜炎耶尔森菌。观察到视网膜和耶尔森氏菌 HSP60 蛋白的交叉反应，还表明口腔内复发性口疮中链球菌的 65kDa HSP 可能与白塞氏病的葡萄膜视网膜炎有关。我们检查了 60 kDa 热休克蛋白家族各个成员的血清抗体滴度及其与免疫系统作用的关系。 HSP60 特别针对白塞氏病，我们从患者口腔口疮边缘分离出链球菌的 HSP60，以及耶尔森菌、视网膜母细胞瘤和牛视网膜的 HSP60，并研究了这些 HSP60 中每一种在 100 倍稀释后的反应。 20 名白塞氏病患者的血清样本，通过 ELISA 检测。 100倍稀释血清的抗链球菌HSP60抗体和抗视网膜HSP60抗体滴度均显着高于健康供体相似稀释血清样本。ELISA抗体滴度测定结果显示，尽管各种HSP60有相同的抗体滴度。它们具有共同的基本抗原性，但对白塞氏病患者血清中的抗 HSP60 抗体的反应性不同。所测试的各种 HSP60 的抗原性存在细微但显着的差异，所有这些 HSP60 都具有共同的基本抗原性，并且分子量大致相同，这表明视网膜和链球菌 HSP 之间的免疫交叉反应以及相关的自身免疫反应可能参与顽固性葡萄膜视网膜炎的发展，特别是白塞氏病。