CORTICOSTEROID HYPERSENSlTIVITY IN THE UVEITIS TREATMENT AND MOLECULAR BIOLOGICAL BACKGROUND

葡萄膜炎治疗中的皮质类固醇超敏反应和分子生物学背景

基本信息

批准号：
11671759
负责人：
USUI Masahiko
金额：
$ 1.79万
依托单位：
Tokyo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671759/
关键词：
Corticosteroids Sensitivity Uveitis BCG

项目摘要

We evaluated the sensitivity for corticosteroids in 12 cases of Vogt-Koyanagi-Harada disease using the cell surface of early activated antigen CD69 ELISA test on lymphocytes. The results showed the clinical course of VKH disease is dependent on the sursceptibility of lymphocytes to corticosterpoid in each patients. Systemic administration of steroids to patients with Behcet's disease in Japan is often followed by a worsening in ocular symptoms. The sensitivity of 7 patients with Behcet's disease to steroids which were administrered after intraocular surgery was measured. 3/7 showed a low steroids sensitivity, and the frequency of postoperative inflammatory episodes and the intraocular pressure were both higher for this group than for the 4 who showed high steroid sensitivity. Ocular symptoms deteriorate in Japanese patients with Behcet's disease after administration of corticosteroids. This phenomenon is not observed in Western patients. Between '51 and '74, the Law in Japan required BCG vaccine every year. Inoculation is performed in a few European countries. Study was conducted to clarify the relationship between BCG and corticosteroid sensitivity. Rats were inoculated with 1 mg of BCG every week until a foot pad reaction became positive. At the age of 15 weeks, spleen cells were cultured with a mixture of PHA and betamethasone for 24 hours. Interleukin 2 RNA gene was measured using a real-time PCR.. IL2 gene expression was not suppressed by steroids in Lewis rats. In WAKH rats, sensitivity changed from sensitive after BCG inoculation and IL2 gene expression increased after the addition of steroids. BCG was considered to be a factor affecting sensitivity.

我们使用淋巴细胞早期活化抗原 CD69 ELISA 测试的细胞表面评估了 12 例 Vogt-Koyanagi-Harada 病患者对皮质类固醇的敏感性。结果显示，VKH 病的临床病程取决于每位患者淋巴细胞对皮质类固醇的敏感性。在日本，白塞氏病患者全身使用类固醇后，眼部症状往往会恶化。测量了 7 名白塞氏病患者对眼内手术后使用的类固醇的敏感性。 3/7的人表现出低类固醇敏感性，并且该组术后炎症发作的频率和眼压均高于表现出高类固醇敏感性的4人。日本白塞氏病患者在服用皮质类固醇后，眼部症状恶化。这种现象在西方患者中没有观察到。 51 年至 74 年间，日本法律要求每年接种卡介苗疫苗。一些欧洲国家进行了接种。进行研究以阐明卡介苗与皮质类固醇敏感性之间的关系。每周给大鼠接种 1 毫克卡介苗，直至足垫反应呈阳性。 15周龄时，将脾细胞与PHA和倍他米松的混合物一起培养24小时。使用实时 PCR 测量白细胞介素 2 RNA 基因。Lewis 大鼠中类固醇不抑制 IL2 基因表达。在WAKH大鼠中，接种BCG后敏感性由敏感变为敏感，并且添加类固醇后IL2基因表达增加。 BCG被认为是影响敏感性的因素。